Anti-GP73 monoclonal antibodies and methods of obtaining the same

ABSTRACT

Disclosed herein are antibodies and methods of using said antibodies to detect Golgi protein 73 (GP73) and fucosylated GP73 in a sample.

TECHNICAL FIELD

The present invention relates to GP73, in particular anti-GP73monoclonal antibodies, an assay involving the use of at least oneantibody (e.g., monoclonal antibody), such as an immunoassay, andrelated amino acid and nucleic acid sequences and vectors and host cellscomprising same.

BACKGROUND

Golgi protein 73 (“GP73”) is a 73-kd resident Golgi membrane protein.GP73 is a type II transmembrane protein with a single N-terminaltransmembrane domain and an extensive C-terminal coiled coil domainlocated on the lumenal surface of the Golgi apparatus. GP73 has beenshown to be up-regulated in hepatocytes in viral and non-viral chronicliver disease, which suggests that the protein may be involved in thecellular disease response of hepatocytes. In addition, GP73 levels arehigher in patients with liver cancer than in healthy individuals.Fucosylated glycosylation has been found in three quarters of secretedGP73 from hepatocellular carcinoma (HCC) patients. Early studies suggestthat GP73 and fucosylated GP73 may be more reliable biomarkers for theearly diagnosis of liver disease than current markers such asalpha-fetoprotein (AFP), which has the disadvantage of producing falsepositive results since AFP is produced under many circumstances,including other liver diseases. In addition, AFP is not present in allpatients with HCC. Furthermore, a previously known anti-GP73 antibody,the 14H4-23 monoclonal antibody (provided by Dr. Anand Mehta, DrexelUniversity School of Medicine), is insensitive to the presence orabsence of a fucose sugar moiety on the GP73 molecule.

In view of the foregoing, it is an object of the present disclosure toprovide anti-GP73 monoclonal antibodies that may be sensitive to thepresence or absence of a fucose sugar moiety on the GP73 molecule andbind to the fucosylated form of GP73, or alternatively, are insensitiveto the presence or absence of a fucose sugar moiety on the GP73 moleculebut have binding affinities sufficient to be used in immunoassays fordetecting GP73 and/or fucosylated GP73. This and other objects andadvantages, as well as inventive features, will become apparent from thedetailed description provided herein.

SUMMARY

The present invention is directed to an isolated antibody or antibodyfragment thereof capable of binding an epitope within the amino acidsequence of VSQENPEMEGPERDQLVIPDGQEEEQEAAGEGR (hGP73 307-339) (SEQ IDNO:101). The isolated antibody or antibody fragment is selected from thegroup consisting of a human antibody, an immunoglobulin molecule, adisulfide linked Fv, a monoclonal antibody, an affinity matured, a scFv,a chimeric antibody, a single domain antibody, a CDR-grafted antibody, adiabody, a humanized antibody, a multispecific antibody, a Fab, a dualspecific antibody, a DVD, a TVD, a Fab′, a bispecific antibody, aF(ab′)2, and a Fv. The isolated antibody or antibody fragment is human.The isolated antibody or antibody fragment comprises a heavy chainimmunoglobulin constant domain selected from the group consisting of ahuman IgM constant domain, a human IgG4 constant domain, a human IgG1constant domain, a human IgE constant domain, a human IgG2 constantdomain, a human IgG3 constant domain, and a human IgA constant domain.

The present invention is directed to an isolated antibody or antibodyfragment thereof capable of binding an epitope within the amino acidsequence of: EQVVEDRPVGGR (hGP73 276-287) (SEQ ID NO:102). The isolatedantibody or antibody fragment is selected from the group consisting of ahuman antibody, an immunoglobulin molecule, a disulfide linked Fv, amonoclonal antibody, an affinity matured, a scFv, a chimeric antibody, asingle domain antibody, a CDR-grafted antibody, a diabody, a humanizedantibody, a multispecific antibody, a Fab, a dual specific antibody, aDVD, a TVD, a Fab′, a bispecific antibody, a F(ab′)2, and a Fv. Theisolated antibody or antibody is human. The isolated antibody orantibody fragment comprises a heavy chain immunoglobulin constant domainselected from the group consisting of a human IgM constant domain, ahuman IgG4 constant domain, a human IgG1 constant domain, a human IgEconstant domain, a human IgG2 constant domain, a human IgG3 constantdomain, and a human IgA constant domain.

The present invention is directed to an isolated antibody or antibodyfragment thereof capable of binding an epitope within the amino acidsequence of: LRGEDDYNMDENEAESETDK (hGP73 344-363) (SEQ ID NO:103). Theantibody is selected from the group consisting of a human antibody, animmunoglobulin molecule, a disulfide linked Fv, a monoclonal antibody,an affinity matured, a scFv, a chimeric antibody, a single domainantibody, a CDR-grafted antibody, a diabody, a humanized antibody, amultispecific antibody, a Fab, a dual specific antibody, a DVD, a TVD, aFab′, a bispecific antibody, a F(ab′)2, and a Fv. The antibody orantibody fragment is human. The antibody or antibody fragment comprisesa heavy chain immunoglobulin constant domain selected from the groupconsisting of a human IgM constant domain, a human IgG4 constant domain,a human IgG1 constant domain, a human IgE constant domain, a human IgG2constant domain, a human IgG3 constant domain, and a human IgA constantdomain.

The present invention is directed to an isolated antibody or antibodyfragment thereof capable of binding an epitope within the amino acidsequence of: ELKKNEFQGELEKQREQLDKIQSSHNFQLESVNK (hGP73 63-96) (SEQ IDNO:104). The antibody is selected from the group consisting of a humanantibody, an immunoglobulin molecule, a disulfide linked Fv, amonoclonal antibody, an affinity matured, a scFv, a chimeric antibody, asingle domain antibody, a CDR-grafted antibody, a diabody, a humanizedantibody, a multispecific antibody, a Fab, a dual specific antibody, aDVD, a TVD, a Fab′, a bispecific antibody, a F(ab′)2, and a Fv. Theantibody or antibody fragment is human. The antibody or antibodyfragment comprises a heavy chain immunoglobulin constant domain selectedfrom the group consisting of a human IgM constant domain, a human IgG4constant domain, a human IgG1 constant domain, a human IgE constantdomain, a human IgG2 constant domain, a human IgG3 constant domain, anda human IgA constant domain.

The present invention is directed to an isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein the binding of the antibody or antibody fragment toGP73 is sensitive to fucosylation of GP73. The antibody is selected fromthe group consisting of a human antibody, an immunoglobulin molecule, adisulfide linked Fv, a monoclonal antibody, an affinity matured, a scFv,a chimeric antibody, a single domain antibody, a CDR-grafted antibody, adiabody, a humanized antibody, a multispecific antibody, a Fab, a dualspecific antibody, a DVD, a TVD, a Fab′, a bispecific antibody, aF(ab′)2, and a Fv. The antibody or antibody fragment is human. Theantibody or antibody fragment comprises a heavy chain immunoglobulinconstant domain selected from the group consisting of a human IgMconstant domain, a human IgG4 constant domain, a human IgG1 constantdomain, a human IgE constant domain, a human IgG2 constant domain, ahuman IgG3 constant domain, and a human IgA constant domain.

The present invention is directed to an isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein the binding of the antibody or antibody fragment toGP73 is insensitive to fucosylation of GP73. The antibody is selectedfrom the group consisting of a human antibody, an immunoglobulinmolecule, a disulfide linked Fv, a monoclonal antibody, an affinitymatured, a scFv, a chimeric antibody, a single domain antibody, aCDR-grafted antibody, a diabody, a humanized antibody, a multispecificantibody, a Fab, a dual specific antibody, a DVD, a TVD, a Fab′, abispecific antibody, a F(ab′)2, and a Fv. The antibody or antibodyfragment is human. The antibody or antibody fragment comprises a heavychain immunoglobulin constant domain selected from the group consistingof a human IgM constant domain, a human IgG4 constant domain, a humanIgG1 constant domain, a human IgE constant domain, a human IgG2 constantdomain, a human IgG3 constant domain, and a human IgA constant domain.

The present invention is directed to an isolated antibody or antibodyfragment thereof which binds to Golgi Protein 73 (“GP73”), wherein theantibody comprises a domain or region selected from the group consistingof: (a) a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:1, (b) a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:5, (c) a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:9, (d) a variablelight domain region comprising the amino acid sequence of SEQ ID NO:13,(e) a variable heavy domain region comprising the amino acid sequence ofSEQ ID NO:17, (f) a variable light domain region comprising the aminoacid sequence of SEQ ID NO:21, (g) a variable heavy domain regioncomprising the amino acid sequence of SEQ ID NO:25, (h) a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:29, (i) avariable heavy domain region comprising the amino acid sequence of SEQID NO:33, (j) a variable light domain region comprising the amino acidsequence of SEQ ID NO:37; (k) a variable heavy domain region comprisingthe amino acid sequence of SEQ ID NO:41; (l) a variable light domainregion comprising the amino acid sequence of SEQ ID NO:45; (m) variableheavy domain comprising the amino acid sequence of SEQ ID NO:1 and avariable light domain region comprising the amino acid sequence of SEQID NO:5, (n) a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:9 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:13, (o) a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:17 and a variable light domainregion comprising the amino acid sequence of SEQ ID NO:21, (p) avariable heavy domain comprising the amino acid sequence of SEQ ID NO:25and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:29, (q) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:33 and a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:37, (r) a variably heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:45, (s) avariable heavy chain comprising a complementarity determining region(CDR)1 comprising the amino acid sequence of SEQ ID NO:2, a CDR2comprising the amino acid sequence of SEQ ID NO:3, and a CDR3 comprisingthe amino acid sequence of SEQ ID NO:4, (t) a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:6, aCDR2 comprising the amino acid sequence of SEQ ID NO:7, and a CDR3comprising the amino acid sequence of SEQ ID NO:8, (u) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and aCDR3 comprising the amino acid sequence of SEQ ID NO:12, (v) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:14, a CDR2 comprising the amino acid sequence of SEQ ID NO:15, anda CDR3 comprising the amino acid sequence of SEQ ID NO:16, (w) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequence ofSEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20, (x) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24, (y) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28, (z) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32, (aa) a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:34, a CDR2 comprising the amino acidsequence of SEQ ID NO:35, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:36, (bb) a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:38, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:39, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:40, (cc) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and CDR3 comprisingthe amino acid sequence of SEQ ID NO:44; (dd) a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:46, aCDR2 comprising the amino acid sequence of SEQ ID NO:47, and a CDR3comprising the amino acid sequence of SEQ ID NO:48; (ee) a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4 and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8, (ff) a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, anda CDR3 comprising the amino acid sequence of SEQ ID NO:12, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequence ofSEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16, (gg) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:18, a CDR2 comprising the amino acidsequence of SEQ ID NO:19, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:20, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:22, a CDR2 comprising the aminoacid sequence of SEQ ID NO:23, and a CDR3 comprising the amino acidsequence of SEQ ID NO:24, (hh) a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2comprising the amino acid sequence of SEQ ID NO:31, and a CDR3comprising the amino acid sequence of SEQ ID NO:32, (ii) a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, anda CDR3 comprising the amino acid sequence of SEQ ID NO:36, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequence ofSEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40, and (jj) a variable heavy domain chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:42, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:43, and a CDR comprising the aminoacid sequence of SEQ ID NO:44, and a variable light domain chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:46, aCDR2 comprising the amino acid sequence of SEQ ID NO:47, and a CDR3comprising the amino acid sequence of SEQ ID NO:48. The antibody isselected from the group consisting of a human antibody, animmunoglobulin molecule, a disulfide linked Fv, a monoclonal antibody,an affinity matured, a scFv, a chimeric antibody, a single domainantibody, a CDR-grafted antibody, a diabody, a humanized antibody, amultispecific antibody, a Fab, a dual specific antibody, a DVD, a TVD, aFab′, a bispecific antibody, a F(ab′)2, and a Fv. The antibody orantibody fragment is human. The antibody or antibody fragment comprisesa heavy chain immunoglobulin constant domain selected from the groupconsisting of a human IgM constant domain, a human IgG4 constant domain,a human IgG1 constant domain, a human IgE constant domain, a human IgG2constant domain, a human IgG3 constant domain, and a human IgA constantdomain. The antibody or antibody fragment comprises a variable heavyregion comprising a sequence selected from the group consisting of SEQID NO:1, SEQ ID NO:9, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:33, and SEQID NO:41. The antibody or antibody fragment comprises a variable lightregion comprising a sequence selected from the group consisting of SEQID NO:5, SEQ ID NO:13, SEQ ID NO:21, SEQ ID NO:29, SEQ ID NO:37, and SEQID NO:45. The antibody or antibody fragment comprises variable heavydomain comprising the amino acid sequence of SEQ ID NO:1 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:5.The antibody or antibody fragment comprises a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:9 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:13. Theantibody or antibody fragment comprises a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:17 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:21. Theantibody or antibody fragment comprises a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:29. Theantibody or antibody fragment comprises a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:33 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:37. Theantibody or antibody fragment comprises a variably heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:45. Theantibody or antibody fragment comprises a variable heavy domain thatcomprises complementarity-determining region (CDR) residues: SEQ IDNO:2, SEQ ID NO:3, and SEQ ID NO:4; SEQ ID NO:10, SEQ ID NO:11, and SEQID NO:12; SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:20; SEQ ID NO:26,SEQ ID NO:27, and SEQ ID NO:28; SEQ ID NO:34, SEQ ID NO:35, and SEQ IDNO:36; or SEQ ID NO:42, SEQ ID NO:43, and SEQ ID NO:44. The antibody orantibody fragment comprises a variable light domain that comprisescomplementarity-determining region (CDR) residues: SEQ ID NO:6, SEQ IDNO:7, and SEQ ID NO:8; SEQ ID NO:14, SEQ ID NO:15, and SEQ ID NO:16; SEQID NO:22, SEQ ID NO:23, and SEQ ID NO:24; SEQ ID NO:30, SEQ ID NO:31,and SEQ ID NO:32; SEQ ID NO:38, SEQ ID NO:39, and SEQ ID NO:40; or SEQID NO:46, SEQ ID NO:47, and SEQ ID NO:48. The antibody or antibodyfragment comprises a variable heavy domain that comprisescomplementarity-determining region (CDR) residues SEQ ID NO:2, SEQ IDNO:3, and SEQ ID NO:4, and a variable light domain that comprisescomplementarity-determining region (CDR) residues SEQ ID NO:6, SEQ IDNO:7, and SEQ ID NO:8. The antibody or antibody fragment comprises avariable heavy domain that comprises complementarity-determining region(CDR) residues SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, and avariable light domain that comprises complementarity-determining region(CDR) residues SEQ ID NO:14, SEQ ID NO:15, and SEQ ID NO:16. Theantibody or antibody fragment comprises a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:18, SEQ ID NO:19, and SEQ ID NO:20, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:22, SEQ ID NO:23, and SEQ ID NO:24. The antibody or antibody fragmentcomprises a variable heavy domain that comprisescomplementarity-determining region (CDR) residues SEQ ID NO:26, SEQ IDNO:27, and SEQ ID NO:28, and a variable light domain that comprisescomplementarity-determining region (CDR) residues SEQ ID NO:30, SEQ IDNO:31, and SEQ ID NO:32. The antibody or antibody fragment comprises avariable heavy domain that comprises complementarity-determining region(CDR) residues SEQ ID NO:34, SEQ ID NO:35, and SEQ ID NO:36, and avariable light domain that comprises complementarity-determining region(CDR) residues SEQ ID NO:38, SEQ ID NO:39, and SEQ ID NO:40. Theantibody or antibody fragment comprises a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:42, SEQ ID NO:43, and SEQ ID NO:44, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:46, SEQ ID NO:47, and SEQ ID NO:48. The antibody or antibody fragmentfurther comprises an agent selected from the group consisting of: animmunoadhesion molecule, an imaging agent, and a therapeutic agent. Theimaging agent is selected from the group consisting of a radiolabel, anenzyme, a fluorescent label, a luminescent label, a bioluminescentlabel, a magnetic label, and biotin. The radiolabel is selected from thegroup consisting of 3H, 14C, 35S, 90Y, 99Tc, 111In, 125I, 131I, 177Lu,166Ho, and 153Sm. The antibody or antibody fragment binds to the GP73epitope within the amino acid sequence of:VSQENPEMEGPERDQLVIPDGQEEEQEAAGEGR (hGP73 307-339) (SEQ ID NO:101). Theantibody or antibody fragment comprises: a variable heavy domain regioncomprising the amino acid sequence of SEQ ID NO:9; a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:13; avariable heavy domain comprising the amino acid sequence of SEQ ID NO:9and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:13; a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:10, a CDR2 comprising the amino acidsequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:12; a variable light chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:14, a CDR2 comprising the amino acidsequence of SEQ ID NO:15, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:16; or a variable heavy chain comprising a CDR 1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12, and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16. The antibody or antibody fragment bindsto the GP73 epitope within the amino acid sequence of: EQVVEDRPVGGR(hGP73 276-287) (SEQ ID NO:102). The antibody or antibody fragmentcomprises: a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:17; a variable light domain region comprising theamino acid sequence of SEQ ID NO:21; a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:17 and a variable light domainregion comprising the amino acid sequence of SEQ ID NO:21; a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, anda CDR3 comprising the amino acid sequence of SEQ ID NO:20; a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, anda CDR3 comprising the amino acid sequence of SEQ ID NO:24; or a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, anda CDR3 comprising the amino acid sequence of SEQ ID NO:20, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequence ofSEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24. The antibody or antibody fragment binds to the GP73 epitopewithin the amino acid sequence of: LRGEDDYNMDENEAESETDK (hGP73 344-363)(SEQ ID NO:103). The antibody or antibody fragment comprises: a variableheavy domain region comprising the amino acid sequence of SEQ ID NO:1; avariable light domain region comprising the amino acid sequence of SEQID NO:5; a variable heavy domain comprising the amino acid sequence ofSEQ ID NO:1 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:5; a variable heavy chain comprising acomplementarity determining region (CDR)1 comprising the amino acidsequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequence ofSEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4; a variable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequence ofSEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; or a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequenceof SEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8. The antibody or antibody fragment binds to the GP73 epitope withinthe amino acid sequence of: ELKKNEFQGELEKQREQLDKIQSSHNFQLESVNK (hGP7363-96) (SEQ ID NO:104). The antibody or antibody fragment comprises: avariable heavy domain region comprising the amino acid sequence of SEQID NO:33; a variable light domain region comprising the amino acidsequence of SEQ ID NO:37; a variable heavy domain comprising the aminoacid sequence of SEQ ID NO:33 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:37; a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, and aCDR3 comprising the amino acid sequence of SEQ ID NO:36; a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:38, a CDR2 comprising the amino acid sequence of SEQ ID NO:39, anda CDR3 comprising the amino acid sequence of SEQ ID NO:40; or a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, anda CDR3 comprising the amino acid sequence of SEQ ID NO:36, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequence ofSEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40. The binding of the antibody or antibody fragment to GP73 issensitive to the presence or absence of a fucose sugar moiety on GP73.The antibody or antibody fragment comprises: a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:25 or SEQ IDNO:33; a variable light domain region comprising the amino acid sequenceof SEQ ID NO:29 or SEQ ID NO:37; a variable heavy domain comprising theamino acid sequence of SEQ ID NO:25 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:29; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:33 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:37;a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence ofSEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:34, a CDR2 comprising the amino acid sequenceof SEQ ID NO:35, and a CDR3 comprising the amino acid sequence of SEQ IDNO:36; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequenceof SEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; or a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:34, a CDR2 comprising the amino acid sequenceof SEQ ID NO:35, and a CDR3 comprising the amino acid sequence of SEQ IDNO:36, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequenceof SEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40. The binding of the antibody or antibody fragment to GP73 isinsensitive to the presence or absence of a fucose sugar moiety on GP73.The antibody or antibody fragment comprises: a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:1, SEQ ID NO:9,SEQ ID NO:17, or SEQ ID NO:41; a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:5, SEQ ID NO:13, SEQ ID NO:21, orSEQ ID NO:45; a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:1 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:5; a variable heavy domain comprising theamino acid sequence of SEQ ID NO:9 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:13; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:17 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:21;a variable heavy domain comprising the amino acid sequence of SEQ IDNO:41 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:45; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:2, a CDR2 comprising theamino acid sequence of SEQ ID NO:3, and a CDR3 comprising the amino acidsequence of SEQ ID NO:4; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:10, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:12; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:18, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:19, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:20; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:42, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:43, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:44; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:6, a CDR2 comprising theamino acid sequence of SEQ ID NO:7, and a CDR3 comprising the amino acidsequence of SEQ ID NO:8; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:22, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:23, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:24; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:46, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:47, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:48; a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:2, a CDR2 comprising theamino acid sequence of SEQ ID NO:3, and a CDR3 comprising the amino acidsequence of SEQ ID NO:4, and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:6, a CDR2 comprising theamino acid sequence of SEQ ID NO:7, and a CDR3 comprising the amino acidsequence of SEQ ID NO:8; a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:10, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:12, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:14, a CDR2comprising the amino acid sequence of SEQ ID NO:15, and a CDR3comprising the amino acid sequence of SEQ ID NO:16; a variable heavychain comprising a CDR 1 comprising the amino acid sequence of SEQ IDNO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, and aCDR3 comprising the amino acid sequence of SEQ ID NO:20, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, anda CDR3 comprising the amino acid sequence of SEQ ID NO:24; or a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, anda CDR3 comprising the amino acid sequence of SEQ ID NO:44, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequence ofSEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48.

The present invention is directed to an isolated antibody thatspecifically binds GP-73 and has a CDR-H1 having the formula:X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:108) a CDR-H2 having the formula:X₁₀-I-X₁₁-X₁₂-X₁₃-X₁₄-X₁₅-X₁₆X₁₇-X₁₈-X₁₉-X₂₀-X₂₁-X₂₂-X₂₃-X₂₄-X₂₅-X₂₆-X₂₇(SEQ ID NO:109), a CDR-H3 having the formula:X₂₈-X₂₉-X₃₀-X₃₁-X₃₂-X₃₃-X₃₄-X₃₅-X₃₆-X₃₇-X₃₈-X₃₉-Y (SEQ ID NO:110), aCDR-L1 having the formula:X₄₀-X₄₁-S-X₄₂-X₄₃-X₄₄-X₄₅-X₄₆-X₄₇-X₄₈-X₄₉-X₅₀-X₅₁-X₅₂-X₅₃-X₅₄ (SEQ IDNO:111), a CDR-L2 having the formula: X₅₅-X₅₆-S-X₅₇-X₅₈-X₅₉-X₆₀ (SEQ IDNO:112) and a CDR-L3 having the formula: X₆₁-Q-X₆₂-X₆₃-X₆₄-X₆₅-P-X₆₆-T(SEQ ID NO:113), wherein: X₅ is S, N or T; X₆ is Y or N; X₇ is W, V, G,A or T; X₈ is I, V or M; X₉ is E, H, S or N; X₁₀ is E, Y, V, T or R; X₁₁is L, W, S or R; X₁₂ is P, S, R or T; X₁₃ is absent or is K; X₁₄ isabsent or is R; X₁₅ is G, T or Y; X₁₆ is S, G or N; X₁₇ is G, D, S, T orY; X₁₈ is N, S, Y, G or T; X₁₉ is T or I; X₂₀ is N, K, Y or F; X₂₁ is Yor F; X₂₂ is N, P or A; X₂₃ is E, S or D; X₂₄ is K, A or S; X₂₅ is F, Lor V; X₂₆ is K or M; X₂₇ is G, S or D; X₂₈ is G, Q, D, E or N; X₂₉ is R,Q, P, Y, W or G; X₃₀ is G, L, F, D or T; X₃₁ is S, T, S or G; X₃₂ is Y,D, G, E or T; X₃₃ is R, Y, D, L or F; X₃₄ is Y, F, T or H; X₃₅ is H orY; X₃₆ is W, D or Y; X₃₇ is F, Y or A; X₃₈ is absent or is F or M; X₃₉is A or D; X₄₀ is K, T, R or S; X₄₁ is A or S; X₄₂ is Q, S or K; X₄₃ isS or G; X₄₄ is V or L; X₄₅ is D or L; X₄₆ is Y, D or H; X₄₇ is absent orS; X₄₈ is D, V, N or I; X₄₉ is G, V or S; X₅₀ is D, K, S or I; X₅₁ is S,T, I or N; X₅₂ is Y or D; X₅₃ is M, L or V; X₅₄ is N, I, S, H or Y; X₅₅is A, L, S, Q or R; X₅₆ is A, V, T or M; X₅₇ is N, K, Y or S; X₅₈ is Lor R; X₅₉ is E, D, Y or A; X₆₀ is S or I; X₆₁ is Q, W, H or A; X₆₂ is S,G, H, Y or N; X₆₃ is N, T, F, H or L; X₆₄ is E, H, T, R or S; X₆₅ is D,F, T, S, L or I; and X₆₆ is Y or L.

The present invention is directed to an isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein said antibody has an equilibrium dissociation constant(KD) of between about 4.0×10-9 to about 1.8×10-12.

The present invention is directed to an isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein said antibody has a dissociation rate (koff) ofbetween about 1.5×10-3 to about 8.0×10-6.

The present invention is directed to an isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein said antibody has an association rate (kon) of betweenabout 1.0×105 to about 4.1×106.

The present invention is directed to an isolated nucleic acid encodingany one of SEQ ID NO:1-48.

The present invention is directed to an isolated nucleic acid encodingthe antibody or antibody fragment of claim 1, 5, 9, 13, 17, 21, 25, 60,61, 62, or 63.

The present invention is directed to an isolated nucleic acid comprisingat least one nucleic acid sequence of SEQ ID NO:49-96.

The present invention is directed to an isolated nucleic acid comprisinga nucleotide sequence encoding: the amino acid sequence of (i) SEQ IDNO:1, (ii) SEQ ID NO:5, or (iii) SEQ ID NO:1 and SEQ ID NO:5, optionallyas part of a vector; the amino acid sequence of (i) SEQ ID NO:9, (ii)SEQ ID NO:13, or (iii) SEQ ID NO:9 and SEQ ID NO:13, optionally as partof a vector; the amino acid sequence of (i) SEQ ID NO:17, (ii) SEQ IDNO:21, or (iii) SEQ ID NO:17 and SEQ ID NO:21, optionally as part of avector; the amino acid sequence of (i) SEQ ID NO:25, (ii) SEQ ID NO:29,or (iii) SEQ ID NO:25 and SEQ ID NO:29, optionally as part of a vector;the amino acid sequence of (i) SEQ ID NO:33, (ii) SEQ ID NO:37, or (iii)SEQ ID NO:33 and SEQ ID NO:37, optionally as part of a vector; or theamino acid sequence of (i) SEQ ID NO:41, (ii) SEQ ID NO:45, or (iii) SEQID NO:41 and SEQ ID NO:45, optionally as part of a vector. The isolatednucleic acid of claim 67, wherein the nucleic acid comprises: thenucleotide sequence of (i) SEQ ID NO:49, (ii) SEQ ID NO:53, or (iii) SEQID NO:49 and SEQ ID NO:53, optionally as part of a vector; thenucleotide sequence of (i) SEQ ID NO:57, (ii) SEQ ID NO:61, or (iii) SEQID NO:57 and SEQ ID NO:61, optionally as part of a vector; thenucleotide sequence of (i) SEQ ID NO:65, (ii) SEQ ID NO:69, or (iii) SEQID NO:65 and SEQ ID NO:69, optionally as part of a vector; thenucleotide sequence of (i) SEQ ID NO:73, (ii) SEQ ID NO:77, or (iii) SEQID NO:73 and SEQ ID NO:77, optionally as part of a vector; thenucleotide sequence of (i) SEQ ID NO:81, (ii) SEQ ID NO:85, or (iii) SEQID NO:81 and SEQ ID NO:85, optionally as part of a vector; or thenucleotide sequence of (i) SEQ ID NO:89, (ii) SEQ ID NO:93, or (iii) SEQID NO:89 and SEQ ID NO:93, optionally as part of a vector.

The present invention is directed to a host cell comprising andexpressing an isolated nucleic acid comprising a nucleotide sequenceencoding: the amino acid sequence of (i) SEQ ID NO:1, (ii) SEQ ID NO:5,or (iii) SEQ ID NO:1 and SEQ ID NO:5, optionally as part of a vector;the amino acid sequence of (i) SEQ ID NO:9, (ii) SEQ ID NO:13, or (iii)SEQ ID NO:9 and SEQ ID NO:13, optionally as part of a vector; the aminoacid sequence of (i) SEQ ID NO:17, (ii) SEQ ID NO:21, or (iii) SEQ IDNO:17 and SEQ ID NO:21, optionally as part of a vector; the amino acidsequence of (i) SEQ ID NO:25, (ii) SEQ ID NO:29, or (iii) SEQ ID NO:25and SEQ ID NO:29, optionally as part of a vector; the amino acidsequence of (i) SEQ ID NO:33, (ii) SEQ ID NO:37, or (iii) SEQ ID NO:33and SEQ ID NO:37, optionally as part of a vector; or the amino acidsequence of (i) SEQ ID NO:41, (ii) SEQ ID NO:45, or (iii) SEQ ID NO:41and SEQ ID NO:45, optionally as part of a vector.

The present invention is directed to a pharmaceutical compositioncomprising the antibody, antibody fragment, mixture or derivativethereof of claim 1, 5, 9, 13, 17, 21, 25, 60, 61, 62, or 63. The presentinvention is directed to a method for determining GP73 concentration ina test sample, the method comprising: contacting the test sample with atleast one capture antibody, wherein the capture antibody binds to anepitope on GP73 or a fragment of GP73 to form a capture antibody-GP73antigen complex; contacting the capture antibody-GP73 antigen complexwith at least one detection antibody comprising a detectable label,wherein the detection antibody binds to an epitope on GP73 that is notbound by the capture antibody and forms a capture antibody-GP73antigen-detection antibody complex; and determining the GP73concentration in the test sample based on the signal generated by thedetectable label in the capture antibody-GP73 antigen-detection antibodycomplex formed in the previous step; wherein the at least one captureantibody comprises the isolated antibody or antibody fragment of claim1, 5, 9, 13, 17, 21, 25, 60, 61, 62, or 63 and the at least onedetection antibody comprises the isolated antibody or fragment antibodyof claim 1, 5, 9, 13, 17, 21, 25, 60, 61, 62, or 63, and wherein theleast one capture antibody is different from the at least one detectionantibody. The method further comprises comparing the signal generated bythe detectable label as a direct or indirect indication of the GP73concentration in the test sample to a signal generated as a direct orindirect indication of the GP73 concentration in a control orcalibrator. The GP73 concentration in the test sample is used todetermine or assess whether a subject has or is at risk of developingliver disease. An increased GP73 concentration as compared to the GP73concentration in a control or calibrator indicates that the subject hasliver disease. The liver disease is liver cirrhosis or liver cancer.

The present invention is directed to a method for determining GP73concentration in a test sample, the method comprising: contacting thetest sample with at least one capture antibody, wherein the captureantibody binds to an epitope on GP73 or a fragment of GP73 to form acapture antibody-GP73 antigen complex; contacting the captureantibody-GP73 antigen complex with at least one detection antibodycomprising a detectable label, wherein the detection antibody binds toan epitope on GP73 that is not bound by the capture antibody and forms acapture antibody-GP73 antigen-detection antibody complex; anddetermining the GP73 concentration in the test sample based on thesignal generated by the detectable label in the capture antibody-GP73antigen-detection antibody complex formed in (b); wherein the at leastone capture antibody comprises: a variable heavy domain comprising theamino acid sequence of SEQ ID NO:41; a variable light domain comprisingthe amino acid sequence of SEQ ID NO:45; a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain comprising the amino acid sequence of SEQ ID NO:45; a variableheavy chain comprising a complementarity determining region (CDR)1comprising the amino acid sequence of SEQ ID NO:42, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:43, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:44; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:46, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:47, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:48; or a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and a CDR3comprising the amino acid sequence of SEQ ID NO:44 and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, and aCDR3 comprising the amino acid sequence of SEQ ID NO:48; wherein the atleast one detection antibody comprises: a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25; a variable lightdomain comprising the amino acid sequence of SEQ ID NO:29; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:25 and avariable light domain comprising the amino acid sequence of SEQ IDNO:29; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; or a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32.

The present invention is directed to a method for determiningfucosylated GP73 concentration in a test sample, the method comprising;contacting the test sample with at least one capture binding protein,wherein the capture binding protein binds to a region of GP73 or afragment of GP73 to form a capture binding protein-GP73 complex;contacting the capture binding protein-GP73 complex with at least onedetection binding protein comprising a detectable label, wherein thedetection binding protein binds to a region of GP73 that is not bound bythe capture binding protein and forms a capture bindingprotein-GP73-detection binding protein complex; and determining the GP73concentration in the test sample based on the signal generated by thedetectable label in the capture binding protein-GP73-detection bindingprotein complex formed in the previous step; wherein the at least onecapture binding protein comprises a protein, antibody or antibodyfragment whose binding to GP73 is sensitive to the presence or absenceof a fucose sugar moiety on GP73 and the at least one detection bindingprotein comprises a protein, antibody or antibody fragment whose bindingto GP73 is insensitive to the presence or absence of a fucose sugarmoiety on GP73.

The present invention is directed to a method for determiningfucosylated GP73 concentration in a test sample, the method comprising:contacting the test sample with at least one capture binding protein,wherein the capture binding protein binds to a region of GP73 or afragment of GP73 to form a capture binding protein-GP73 complex;contacting the capture binding protein-GP73 complex with at least onedetection binding protein comprising a detectable label, wherein thedetection binding protein binds to a region of GP73 that is not bound bythe capture binding protein and forms a capture bindingprotein-GP73-detection binding protein complex; and determining the GP73concentration in the test sample based on the signal generated by thedetectable label in the capture binding protein-GP73-detection bindingprotein complex formed in the previous step; wherein the at least onecapture binding protein comprises a protein, antibody or antibodyfragment whose binding to GP73 is insensitive to the presence or absenceof a fucose sugar moiety on GP73 and the at least one detection bindingprotein comprises a protein, antibody or antibody fragment whose bindingto GP73 is sensitive the presence or absence of a fucose sugar moiety onGP73. The protein, antibody or antibody fragment whose binding to GP73is sensitive to the presence or absence of a fucose sugar moiety on GP73comprises: Aleuria aurantia lectin (AAL) or a fragment thereof; avariable heavy domain region comprising the amino acid sequence of SEQID NO:25 or SEQ ID NO:33; a variable light domain region comprising theamino acid sequence of SEQ ID NO:29 or SEQ ID NO:37; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:25 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:29;a variable heavy domain comprising the amino acid sequence of SEQ IDNO:33 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:37; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:30, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:31, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:32; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:38, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:39, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:40; a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2comprising the amino acid sequence of SEQ ID NO:31, and a CDR3comprising the amino acid sequence of SEQ ID NO:32; or a variable heavychain comprising a CDR 1 comprising the amino acid sequence of SEQ IDNO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, and aCDR3 comprising the amino acid sequence of SEQ ID NO:36, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:38, a CDR2 comprising the amino acid sequence of SEQ ID NO:39, anda CDR3 comprising the amino acid sequence of SEQ ID NO:40. The protein,antibody or antibody fragment whose binding to GP73 is insensitive tothe presence or absence of a fucose sugar moiety on GP73 comprises: avariable heavy domain region comprising the amino acid sequence of SEQID NO:1, SEQ ID NO:9, SEQ ID NO:17, or SEQ ID NO:41; a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:5, SEQ IDNO:13, SEQ ID NO:21, or SEQ ID NO:45; a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:1 and a variable light domainregion comprising the amino acid sequence of SEQ ID NO:5; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:9 and avariable light domain region comprising the amino acid sequence of SEQID NO:13; a variable heavy domain comprising the amino acid sequence ofSEQ ID NO:17 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:21; a variable heavy domain comprising theamino acid sequence of SEQ ID NO:41 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:45; a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4; a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and aCDR3 comprising the amino acid sequence of SEQ ID NO:12; a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, anda CDR3 comprising the amino acid sequence of SEQ ID NO:20; a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, anda CDR3 comprising the amino acid sequence of SEQ ID NO:44; a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8; a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:14, a CDR2 comprising the amino acid sequence of SEQ ID NO:15, and aCDR3 comprising the amino acid sequence of SEQ ID NO:16; a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, anda CDR3 comprising the amino acid sequence of SEQ ID NO:24; a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, anda CDR3 comprising the amino acid sequence of SEQ ID NO:48; a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8; a variable heavychain comprising a CDR 1 comprising the amino acid sequence of SEQ IDNO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and aCDR3 comprising the amino acid sequence of SEQ ID NO:12, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:14, a CDR2 comprising the amino acid sequence of SEQ ID NO:15, anda CDR3 comprising the amino acid sequence of SEQ ID NO:16; a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, anda CDR3 comprising the amino acid sequence of SEQ ID NO:20, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequence ofSEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; or a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48. The method further comprises comparing the signal generated bythe detectable label as a direct or indirect indication of the GP73concentration in the test sample to a signal generated as a direct orindirect indication of the GP73 concentration in a control orcalibrator. The GP73 concentration in the test sample is used todetermine or assess whether a subject has or is at risk of developingliver disease. An increased GP73 concentration as compared to the GP73concentration in a control or calibrator indicates that the subject hasliver disease. The liver disease is liver cirrhosis or liver cancer.

The present invention is directed to a method of diagnosing and treatingliver disease in a subject, the method comprising: obtaining abiological sample comprising blood from the subject; determining theGP73 concentration in the biological sample from the subject using themethod of claim 81; comparing the GP73 concentration in the biologicalsample with the GP73 concentration in a normal control or calibrator;diagnosing the subject as having liver disease if the GP73 concentrationin the biological sample is greater than the GP73 concentration in thenormal control or calibrator; and administering a liver diseasetreatment regimen to the subject diagnosed as having liver disease. Thebiological sample of a subject is selected from a tissue sample, bodilyfluid, whole blood, plasma, serum, urine, bronchoalveolar lavage fluid,and a cell culture suspension or fraction thereof. The biological sampleof a subject is blood plasma or blood serum. The liver disease is livercirrhosis or liver cancer. The method further comprises determining thelevel of at least one additional biomarker of liver disease in thebiological sample, and comparing the level of the at least oneadditional biomarker of liver disease to a reference concentration valuefor the at least one biomarker of liver disease. The additionalbiomarker of liver disease is selected from the group consisting ofPIVKA-II, AFP, AFP-L3, Fuc-HPX, Fc-Kin and F-AT.

The present invention is directed to a method for determining if asubject is responding to the administration of one or morepharmaceutical compositions, the method comprising: measuring the GP73concentration in a sample from the subject using the method of claim 81;comparing the GP73 concentration in the sample with the GP73concentration in a normal control or calibrator, wherein an altered GP73concentration indicates that the subject is not responding to theadministration of one or more pharmaceutical compositions; and adjustingthe treatment of the subject if the subject is not responding to theadministration of one or more pharmaceutical compositions.

The present invention is directed to a method of diagnosing and treatingliver disease in a subject, the method comprising: contacting the testsample with at least one capture antibody, wherein the capture antibodybinds to an epitope on GP73 or a fragment of GP73 to form a captureantibody-GP73 antigen complex; contacting the capture antibody-GP73antigen complex with at least one detection antibody comprising adetectable label, wherein the detection antibody binds to an epitope onGP73 that is not bound by the capture antibody and forms a captureantibody-GP73 antigen-detection antibody complex; determining the GP73concentration in the test sample based on the signal generated by thedetectable label in the capture antibody-GP73 antigen-detection antibodycomplex formed in (b); comparing the GP73 concentration in the samplewith the GP73 concentration in a normal control or calibrator;diagnosing the subject as having liver disease if the GP73 concentrationin the biological sample is greater than the GP73 concentration in thenormal control or calibrator; and administering a liver diseasetreatment regimen to the subject diagnosed as having liver disease;wherein the at least one capture antibody comprises: a variable heavydomain comprising the amino acid sequence of SEQ ID NO:41; a variablelight domain comprising the amino acid sequence of SEQ ID NO:45; avariable heavy domain comprising the amino acid sequence of SEQ ID NO:41and a variable light domain comprising the amino acid sequence of SEQ IDNO:45; a variable heavy chain comprising a complementarity determiningregion (CDR)1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and a CDR3comprising the amino acid sequence of SEQ ID NO:44; a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, and aCDR3 comprising the amino acid sequence of SEQ ID NO:48; or a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, anda CDR3 comprising the amino acid sequence of SEQ ID NO:44 and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, anda CDR3 comprising the amino acid sequence of SEQ ID NO:48; wherein theat least one detection antibody comprises: a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25; a variable lightdomain comprising the amino acid sequence of SEQ ID NO:29; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:25 and avariable light domain comprising the amino acid sequence of SEQ IDNO:29; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; or a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32. The liver disease is liver cirrhosis or liver cancer.

The present invention is directed to a kit comprising the antibody,antibody fragment, mixture or derivative thereof of claim 1, 5, 9, 13,17, 21, 25, 60, 61, 62, or 63.

The present invention is directed to a kit comprising an isolatednucleic acid encoding the antibody or antibody fragment of claim 1, 5,9, 13, 17, 21, 25, 60, 61, 62, or 63.

The present invention is directed to a kit for assaying a test samplefor GP73, which kit comprises at least one capture antibody, wherein thecapture antibody binds to an epitope on GP73 or a fragment of GP73, atleast one detection antibody, wherein the detection antibody binds to anepitope on GP73 that is not bound by the capture antibody, andinstructions for assaying the test sample for GP73, wherein the at leastone capture antibody comprises the isolated antibody or antibodyfragment of claim 1, 5, 9, 13, 17, 21, 25, 60, 61, 62, or 63 and the atleast one detection antibody comprises the isolated antibody or antibodyfragment of claim 1, 5, 9, 13, 17, 21, 25, 60, 61, 62, or 63, andwherein the least one capture antibody is different from the at leastone detection antibody. The kit further comprises a reference standardindicating a GP73 concentration in a control or calibrator.

The present invention is directed to a kit for assaying a test samplefor GP73, which kit comprises at least one capture antibody, wherein thecapture antibody binds to an epitope on GP73 or a fragment of GP73, atleast one detection antibody, wherein the detection antibody binds to anepitope on GP73 that is not bound by the capture antibody, andinstructions for assaying the test sample for GP73, wherein the at leastone capture antibody comprises: a variable heavy domain comprising theamino acid sequence of SEQ ID NO:41; a variable light domain comprisingthe amino acid sequence of SEQ ID NO:45; a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain comprising the amino acid sequence of SEQ ID NO:45; a variableheavy chain comprising a complementarity determining region (CDR)1comprising the amino acid sequence of SEQ ID NO:42, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:43, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:44; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:46, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:47, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:48; or a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and a CDR3comprising the amino acid sequence of SEQ ID NO:44 and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, and aCDR3 comprising the amino acid sequence of SEQ ID NO:48; wherein the atleast one detection antibody comprises: a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25; a variable lightdomain comprising the amino acid sequence of SEQ ID NO:29; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:25 and avariable light domain comprising the amino acid sequence of SEQ IDNO:29; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; or a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32, wherein the at least one detection antibody is optionallydetectably labeled.

The present invention is directed to a kit for assaying a test samplefor fucosylated GP73, which kit comprises at least one capture bindingprotein, wherein the capture binding protein binds to GP73 or a fragmentof GP73, at least one detection binding protein, wherein the detectionbinding protein binds to GP73 that is not bound by the capture bindingprotein, and instructions for assaying the test sample for GP73, whereinthe at least one capture binding protein comprises a protein, antibodyor antibody fragment whose binding to GP73 is insensitive to thepresence or absence of a fucose sugar moiety on GP73 and the at leastone detection binding protein comprises a protein, antibody or antibodyfragment whose binding to GP73 is sensitive to fucosylation of GP73.

The present invention is directed to a kit for assaying a test samplefor fucosylated GP73, which kit comprises at least one capture bindingprotein, wherein the capture binding protein binds to GP73 or a fragmentof GP73, at least one detection binding protein, wherein the detectionbinding protein binds to GP73 that is not bound by the capture bindingprotein, and instructions for assaying the test sample for GP73, whereinthe at least one capture binding protein comprises a protein, antibodyor antibody fragment whose binding to GP73 is sensitive to fucosylationof GP73 and the at least one detection binding protein comprises aprotein, antibody or antibody fragment whose binding to GP73 isinsensitive to fucosylation of GP73. The protein, antibody or antibodyfragment whose binding to GP73 is sensitive to fucosylation of GP73comprises: Aleuria aurantia lectin (AAL) or a fragment thereof; avariable heavy domain region comprising the amino acid sequence of SEQID NO:25 or SEQ ID NO:33; a variable light domain region comprising theamino acid sequence of SEQ ID NO:29 or SEQ ID NO:37; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:25 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:29;a variable heavy domain comprising the amino acid sequence of SEQ IDNO:33 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:37; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:30, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:31, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:32; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:38, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:39, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:40; a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2comprising the amino acid sequence of SEQ ID NO:31, and a CDR3comprising the amino acid sequence of SEQ ID NO:32; or a variable heavychain comprising a CDR 1 comprising the amino acid sequence of SEQ IDNO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, and aCDR3 comprising the amino acid sequence of SEQ ID NO:36, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:38, a CDR2 comprising the amino acid sequence of SEQ ID NO:39, anda CDR3 comprising the amino acid sequence of SEQ ID NO:40. The protein,antibody or antibody fragment whose binding to GP73 is insensitive tofucosylation of GP73 comprises a variable heavy domain region comprisingthe amino acid sequence of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:17, orSEQ ID NO:41; a variable light domain region comprising the amino acidsequence of SEQ ID NO:5, SEQ ID NO:13, SEQ ID NO:21, or SEQ ID NO:45; avariable heavy domain comprising the amino acid sequence of SEQ ID NO:1and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:5; a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:9 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:13; a variable heavy domain comprising theamino acid sequence of SEQ ID NO:17 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:21; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:41 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:45;a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequence ofSEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4; a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:10, a CDR2 comprising the amino acid sequence ofSEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ IDNO:12; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequenceof SEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; a variable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequence ofSEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequenceof SEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:10, a CDR2 comprising the amino acid sequenceof SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ IDNO:12, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequenceof SEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequenceof SEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; or a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48. The kit further comprises a reference standard indicating a GP73concentration in a control or calibrator.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the E. coli GP73 construct.

FIG. 2 shows the CHO and HEK GP73-hFc construct.

FIG. 3 shows a competitive inhibition assay of the biotin labeledanti-GP73 mAb with free AAL.

FIG. 4 shows the reactivity of anti-GP73 mAb with mouse-human chimericIgG, GP73 expressed in E. coli, and GP73-hFc recombinant antigenexpressed in HEK cells.

FIG. 5 shows a competitive inhibition assay of the biotin labeledanti-GP73 mAb with free fucose.

FIG. 6 shows the mAb 1A-3187 reactivity with periodated GP73.

FIG. 7 shows the mAb 1A-4246 reactivity with periodated GP73.

FIG. 8 shows the mAb 1B-3246 reactivity with periodated GP73.

FIG. 9 shows the mAb 1B-3440 reactivity with periodated GP73.

FIG. 10 shows the mAb 1B-4863 reactivity with periodated GP73.

FIG. 11 shows the mAb 1B-4971 reactivity with periodated GP73.

FIG. 12 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 12A) and light chain sequences (FIG. 12B) formAb 1A-3187.

FIG. 13 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 13A) and light chain sequences (FIG. 13B) formAb 1A-4246.

FIG. 14 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 14A) and light chain sequences (FIG. 14B) formAb 1B-3246.

FIG. 15 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 15A) and light chain sequences (FIG. 15B) formAb 1B-3440.

FIG. 16 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 16A) and light chain sequences (FIG. 16B) formAb 1B-4863.

FIG. 17 shows a diagram and the nucleotide and amino acid sequences ofthe variable heavy (FIG. 17A) and light chain sequences (FIG. 17B) formAb 1B-4971.

FIG. 18 shows the epitope peptide map of anti-GP73 mAbs for GP73-hFc.

FIG. 19 shows an example of ARCHITECT® sandwich immunoassay principle.

FIG. 20 shows the GP73 ARCHITECT® assay using mAb 14H4-23 or 1A-4246 asthe capture antibody (0.1 mg/mL) and 1B-4863 as the detection antibody(0.5 nM).

FIG. 21 shows the GP73 ARCHITECT® assay using mAb 1A-4246 (0.2 mg/mL) asthe capture antibody and 1B-4863 as the detection antibody (0.5 or 2nM).

DETAILED DESCRIPTION

The present invention relates to anti-Golgi protein 73 (GP73) antibodiesand the use of said antibodies in immunoassays for analyzing the levelsof GP73 and fucosylated GP73 to identify, diagnose and treat diseases insubjects in need thereof. The anti-GP73 antibodies of the presentinvention have higher binding affinities than previously availableantibodies. The antibodies of the present invention can be used inimmunoassays for detecting GP73 and/or fucosylated GP73 thus providing aunique combination of antibodies to detect GP73 and/or fucosylated GP73in a sample over a wider range of concentrations. The use of theseantibodies in such immunoassays provides a more versatile and sensitiveassay. It has also been found that the antibodies of the presentinvention outperformed a known anti-GP73 antibody by about three-fold inimmunoassays.

The increased detectable range of concentration of the disclosedimmunoassays provides a more accurate and sensitive assay for diagnosingand distinguishing liver disease and cancer in a patient. Thus, thedisclosed immunoassays may be used to detect increased or decreased GP73concentrations in a sample compared to a control or calibrator sampleand thus be used to identify various diseases in a patient, such asliver diseases and cancers. The use of the GP73 immunoassay may provideaccurate diagnosing and subsequent treatment of patients with diseases,such as liver disease or cancer.

Section headings as used in this section and the entire disclosureherein are merely for organizational purposes and are not intended to belimiting.

1. DEFINITIONS

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art. In case of conflict, the present document, includingdefinitions, will control. Preferred methods and materials are describedbelow, although methods and materials similar or equivalent to thosedescribed herein can be used in practice or testing of the presentinvention. All publications, patent applications, patents and otherreferences mentioned herein are incorporated by reference in theirentirety. The materials, methods, and examples disclosed herein areillustrative only and not intended to be limiting.

The terms “comprise(s),” “include(s),” “having,” “has,” “can,”“contain(s),” and variants thereof, as used herein, are intended to beopen-ended transitional phrases, terms, or words that do not precludethe possibility of additional acts or structures. The singular forms“a,” “and” and “the” include plural references unless the contextclearly dictates otherwise. The present disclosure also contemplatesother embodiments “comprising,” “consisting of” and “consistingessentially of,” the embodiments or elements presented herein, whetherexplicitly set forth or not.

“14H4-23”, “14H4-23 monoclonal antibody” and “monoclonal antibody14H4-23” as used herein interchangeably refer to a mouse monoclonalantibody which binds to GP73. The 14H4-23 monoclonal antibody was fromthe lab of Drs. Anand Mehta and Tim Block at Drexel University Collegeof Medicine. The binding of the 14H4-23 monoclonal antibody is notsensitive to presence or absence of a fucose sugar moiety on GP73.

“Affinity Matured Antibody” is used herein to refer to an antibody withone or more alterations in one or more CDRs, which result in animprovement in the affinity (i.e. K_(D), k_(d) or k_(a)) of the antibodyfor a target antigen compared to a parent antibody, which does notpossess the alteration(s). Exemplary affinity matured antibodies willhave nanomolar or even picomolar affinities for the target antigen. Avariety of procedures for producing affinity matured antibodies is knownin the art, including the screening of a combinatory antibody librarythat has been prepared using bio-display. For example, Marks et al.,BioTechnology, 10: 779-783 (1992) describes affinity maturation by VHand VL domain shuffling. Random mutagenesis of CDR and/or frameworkresidues is described by Barbas et al., Proc. Nat. Acad. Sci. USA, 91:3809-3813 (1994); Schier et al., Gene, 169: 147-155 (1995); Yelton etal., J. Immunol., 155: 1994-2004 (1995); Jackson et al., J. Immunol.,154(7): 3310-3319 (1995); and Hawkins et al, J. Mol. Biol., 226: 889-896(1992). Selective mutation at selective mutagenesis positions and atcontact or hypermutation positions with an activity-enhancing amino acidresidue is described in U.S. Pat. No. 6,914,128 B1.

“Aleuria aurantia lectin” and “AAL” as used herein interchangeablyrefers to a fungal protein composed of two identical 312-amino acidsubunits that specifically recognizes fucosylated glycans, and is widelyused as a specific probe for fucose. AAL binds preferentially to fucoselinked (α-1,6) to N-acetylglucosamine or to fucose linked (α-1,3) toN-acetyllactosamine related structures.

“Antibody” and “antibodies” as used herein refers to monoclonalantibodies, multispecific antibodies, human antibodies, humanizedantibodies (fully or partially humanized), animal antibodies such as,but not limited to, a bird (for example, a duck or a goose), a shark, awhale, and a mammal, including a non-primate (for example, a cow, a pig,a camel, a llama, a horse, a goat, a rabbit, a sheep, a hamster, aguinea pig, a cat, a dog, a rat, a mouse, etc.) or a non-human primate(for example, a monkey, a chimpanzee, etc.), recombinant antibodies,chimeric antibodies, single-chain Fvs (“scFv”), single chain antibodies,single domain antibodies, Fab fragments, F(ab′) fragments, F(ab′)2fragments, disulfide-linked Fvs (“sdFv”), and anti-idiotypic (“anti-Id”)antibodies, dual-domain antibodies, dual variable domain (DVD) or triplevariable domain (TVD) antibodies (dual-variable domain immunoglobulinsand methods for making them are described in Wu, C., et al., NatureBiotechnology, 25(11):1290-1297 (2007) and PCT International ApplicationWO 2001/058956, the contents of each of which are herein incorporated byreference), and functionally active epitope-binding fragments of any ofthe above. In particular, antibodies include immunoglobulin moleculesand immunologically active fragments of immunoglobulin molecules,namely, molecules that contain an analyte-binding site. Immunoglobulinmolecules can be of any type (for example, IgG, IgE, IgM, IgD, IgA, andIgY), class (for example, IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2), orsubclass. For simplicity sake, an antibody against an analyte isfrequently referred to herein as being either an “anti-analyte antibody”or merely an “analyte antibody” (e.g., an anti-GP73 antibody or a GP73antibody).

“Antibody fragment” as used herein refers to a portion of an intactantibody comprising the antigen-binding site or variable region. Theportion does not include the constant heavy chain domains (i.e. CH2,CH3, or CH4, depending on the antibody isotype) of the Fc region of theintact antibody. Examples of antibody fragments include, but are notlimited to, Fab fragments, Fab′ fragments, Fab′-SH fragments, F(ab′)2fragments, Fd fragments, Fv fragments, diabodies, single-chain Fv (scFv)molecules, single-chain polypeptides containing only one light chainvariable domain, single-chain polypeptides containing the three CDRs ofthe light-chain variable domain, single-chain polypeptides containingonly one heavy chain variable region, and single-chain polypeptidescontaining the three CDRs of the heavy chain variable region.

The “area under curve” or “AUC” refers to area under a ROC curve. AUCunder a ROC curve is a measure of accuracy. An area of 1 represents aperfect test, whereas an area of 0.5 represents an insignificant test. Apreferred AUC may be at least approximately 0.700, at leastapproximately 0.750, at least approximately 0.800, at leastapproximately 0.850, at least approximately 0.900, at leastapproximately 0.910, at least approximately 0.920, at leastapproximately 0.930, at least approximately 0.940, at leastapproximately 0.950, at least approximately 0.960, at leastapproximately 0.970, at least approximately 0.980, at leastapproximately 0.990, or at least approximately 0.995.

“Binding Constants” are described herein. The term “association rateconstant,” “k_(on)” or “k_(a)” as used herein, refers to the valueindicating the binding rate of an antibody to its target antigen or therate of complex formation between an antibody and antigen as shown bythe equation below:

Antibody(Ab)+Antigen(Ag)→Ab-Ag.

The term “dissociation rate constant,” “k_(off)” or “k_(d)” as usedinterchangeably herein, refers to the value indicating the dissociationrate of an antibody form its target antigen or separation of Ab-Agcomplex over time into free antibody and antigen as shown by theequation below:

Antibody(Ab)+Antigen(Ag)←Ab-Ag.

Methods for determining association and dissociation rate constants arewell known in the art. Using fluorescence-based techniques offers highsensitivity and the ability to examine samples in physiological buffersat equilibrium. Other experimental approaches and instruments such as aBIAcore® (biomolecular interaction analysis) assay can be used (e.g.,instrument available from BIAcore International AB, a GE Healthcarecompany, Uppsala, Sweden). Additionally, a KinExA® (Kinetic ExclusionAssay) assay, available from Sapidyne Instruments (Boise, Id.) can alsobe used.

The term “equilibrium dissociation constant”, “Kd”, “K_(d)” or “K_(D)”as used interchangeably, herein, refers to the value obtained bydividing the dissociation rate (koff) by the association rate (kon). Theassociation rate, the dissociation rate and the equilibrium dissociationconstant are used to represent the binding affinity of an antibody to anantigen.

“Binding Protein” is used herein to refer to a monomeric or multimericprotein that binds to and forms a complex with a binding partner, suchas, for example, a polypeptide, an antigen, a chemical compound or othermolecule, or a substrate of any kind. A binding protein specificallybinds a binding partner. Binding proteins include antibodies, as well asantigen-binding fragments thereof and other various forms andderivatives thereof as are known in the art and described herein below,and other molecules comprising one or more antigen-binding domains thatbind to an antigen molecule or a particular site (epitope) on theantigen molecule. Accordingly, a binding protein includes, but is notlimited to, an antibody a tetrameric immunoglobulin, an IgG molecule, anIgG1 molecule, a monoclonal antibody, a chimeric antibody, a CDR-graftedantibody, a humanized antibody, an affinity matured antibody, andfragments of any such antibodies that retain the ability to bind to anantigen.

“Bispecific antibody” is used herein to refer to a full-length antibodythat is generated by quadroma technology (see Milstein et al., Nature,305(5934): 537-540 (1983)), by chemical conjugation of two differentmonoclonal antibodies (see, Staerz et al., Nature, 314(6012): 628-631(1985)), or by knob-into-hole or similar approaches, which introducemutations in the Fc region (see Holliger et al., Proc. Natl. Acad. Sci.USA, 90(14): 6444-6448 (1993)), resulting in multiple differentimmunoglobulin species of which only one is the functional bispecificantibody. A bispecific antibody binds one antigen (or epitope) on one ofits two binding arms (one pair of HC/LC), and binds a different antigen(or epitope) on its second arm (a different pair of HC/LC). By thisdefinition, a bispecific antibody has two distinct antigen-binding arms(in both specificity and CDR sequences), and is monovalent for eachantigen to which it binds to.

For the recitation of numeric ranges herein, each intervening numberthere between with the same degree of precision is explicitlycontemplated. For example, for the range of 6-9, the numbers 7 and 8 arecontemplated in addition to 6 and 9, and for the range 6.0-7.0, thenumber 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, and 7.0 areexplicitly contemplated.

“Cancer” as used herein refers to the uncontrolled and unregulatedgrowth of abnormal cells in the body. Cancerous cells are also calledmalignant cells. Cancer may invade nearby parts of the body and may alsospread to more distant parts of the body through the lymphatic system orbloodstream. Cancers include Adrenocortical Carcinoma, Anal Cancer,Bladder Cancer, Brain Tumor, Breast Cancer, Carcinoid Tumor,Gastrointestinal, Carcinoma of Unknown Primary, Cervical Cancer, ColonCancer, Endometrial Cancer, Esophageal Cancer, Extrahepatic Bile DuctCancer, Ewings Family of Tumors (PNET), Extracranial Germ Cell Tumor,Intraocular Melanoma Eye Cancer, Gallbladder Cancer, Gastric Cancer(Stomach), Extragonadal Germ Cell Tumor, Gestational TrophoblasticTumor, Head and Neck Cancer, Hypopharyngeal Cancer, Islet CellCarcinoma, Kidney Cancer (renal cell cancer), Laryngeal Cancer, AcuteLymphoblastic Leukemia, Leukemia, Acute Myeloid, Chronic LymphocyticLeukemia, Chronic Myelogenous Leukemia, Hairy Cell Leukemia, Lip andOral Cavity Cancer, Liver Cancer, Non-Small Cell Lung Cancer, Small CellLung Cancer, AIDS-Related Lymphoma, Central Nervous System (Primary)Lymphoma, Cutaneous T-Cell Lymphoma, Hodgkin's Disease Lymphoma,Non-Hodgkin's Disease Lymphoma, Malignant Mesothelioma, Melanoma, MerkelCell Carcinoma, Metasatic Squamous Neck Cancer with Occult Primary,Multiple Myeloma and Other Plasma Cell Neoplasms, Mycosis Fungoides,Myelodysplastic Syndrome, Myeloproliferative Disorders, NasopharyngealCancer, euroblastoma, Oral Cancer, Oropharyngeal Cancer, Osteosarcoma,Ovarian Epithelial Cancer, Ovarian Germ Cell Tumor, Pancreatic Cancer,Exocrine, Pancreatic Cancer, Islet Cell Carcinoma, Paranasal Sinus andNasal Cavity Cancer, Parathyroid Cancer, Penile Cancer, PituitaryCancer, Plasma Cell Neoplasm, Prostate Cancer, Rhabdomyosarcoma, RectalCancer, Renal Cell Cancer (cancer of the kidney), Transitional CellRenal Pelvis and Ureter, Salivary Gland Cancer, Sezary Syndrome, SkinCancer, Small Intestine Cancer, Soft Tissue Sarcoma, Testicular Cancer,Malignant Thymoma, Thyroid Cancer, Urethral Cancer, Uterine Cancer,Unusual Cancer of Childhood, Vaginal Cancer, Vulvar Cancer, and Wilms'Tumor.

“CDR” is used herein to refer to the “complementarity determiningregion” within an antibody variable sequence. There are three CDRs ineach of the variable regions of the heavy chain and the light chain,which are designated “CDR1”, “CDR2”, and “CDR3”, for each of thevariable regions. The term “CDR set” as used herein refers to a group ofthree CDRs that occur in a single variable region that binds theantigen. The exact boundaries of these CDRs have been defineddifferently according to different systems. The system described byKabat (Kabat et al., Sequences of Proteins of Immunological Interest(National Institutes of Health, Bethesda, Md. (1987) and (1991)) notonly provides an unambiguous residue numbering system applicable to anyvariable region of an antibody, but also provides precise residueboundaries defining the three CDRs. These CDRs may be referred to as“Kabat CDRs”. Chothia and coworkers (Chothia and Lesk, J. Mol. Biol.,196: 901-917 (1987); and Chothia et al., Nature, 342: 877-883 (1989))found that certain sub-portions within Kabat CDRs adopt nearly identicalpeptide backbone conformations, despite having great diversity at thelevel of amino acid sequence. These sub-portions were designated as“L1”, “L2”, and “L3”, or “H1”, “H2”, and “H3”, where the “L” and the “H”designate the light chain and the heavy chain regions, respectively.These regions may be referred to as “Chothia CDRs”, which haveboundaries that overlap with Kabat CDRs. Other boundaries defining CDRsoverlapping with the Kabat CDRs have been described by Padlan, FASEB J.,9: 133-139 (1995), and MacCallum, J. Mol. Biol., 262(5): 732-745 (1996).Still other CDR boundary definitions may not strictly follow one of theherein systems, but will nonetheless overlap with the Kabat CDRs,although they may be shortened or lengthened in light of prediction orexperimental findings that particular residues or groups of residues oreven entire CDRs do not significantly impact antigen binding. Themethods used herein may utilize CDRs defined according to any of thesesystems, although certain embodiments use Kabat- or Chothia-definedCDRs.

“Component,” “components,” or “at least one component,” refer generallyto a capture antibody, a detection or conjugate a calibrator, a control,a sensitivity panel, a container, a buffer, a diluent, a salt, anenzyme, a co-factor for an enzyme, a detection reagent, a pretreatmentreagent/solution, a substrate (e.g., as a solution), a stop solution,and the like that can be included in a kit for assay of a test sample,such as a patient urine, serum or plasma sample, in accordance with themethods described herein and other methods known in the art. Somecomponents can be in solution or lyophilized for reconstitution for usein an assay.

“Derivative” of an antibody as used herein may refer to an antibodyhaving one or more modifications to its amino acid sequence whencompared to a genuine or parent antibody and exhibit a modified domainstructure. The derivative may still be able to adopt the typical domainconfiguration found in native antibodies, as well as an amino acidsequence, which is able to bind to targets (antigens) with specificity.Typical examples of antibody derivatives are antibodies coupled to otherpolypeptides, rearranged antibody domains, or fragments of antibodies.The derivative may also comprise at least one further compound, e.g. aprotein domain, said protein domain being linked by covalent ornon-covalent bonds. The linkage can be based on genetic fusion accordingto the methods known in the art. The additional domain present in thefusion protein comprising the antibody employed in accordance with theinvention may preferably be linked by a flexible linker, advantageouslya peptide linker, wherein said peptide linker comprises plural,hydrophilic, peptide-bonded amino acids of a length sufficient to spanthe distance between the C-terminal end of the further protein domainand the N-terminal end of the antibody or vice versa. The antibody maybe linked to an effector molecule having a conformation suitable forbiological activity or selective binding to a solid support, abiologically active substance (e.g. a cytokine or growth hormone), achemical agent, a peptide, a protein, or a drug, for example.

“Dual-specific antibody” is used herein to refer to a full-lengthantibody that can bind two different antigens (or epitopes) in each ofits two binding arms (a pair of HC/LC) (see PCT publication WO02/02773). Accordingly, a dual-specific binding protein has twoidentical antigen binding arms, with identical specificity and identicalCDR sequences, and is bivalent for each antigen to which it binds.

“Dual variable domain” is used herein to refer to two or more antigenbinding sites on a binding protein, which may be divalent (two antigenbinding sites), tetravalent (four antigen binding sites), or multivalentbinding proteins. DVDs may be monospecific, i.e., capable of binding oneantigen (or one specific epitope), or multispecific, i.e., capable ofbinding two or more antigens (i.e., two or more epitopes of the sametarget antigen molecule or two or more epitopes of different targetantigens). A preferred DVD binding protein comprises two heavy chain DVDpolypeptides and two light chain DVD polypeptides and is referred to asa “DVD immunoglobulin” or “DVD-Ig”. Such a DVD-Ig binding protein isthus tetrameric and reminiscent of an IgG molecule, but provides moreantigen binding sites than an IgG molecule. Thus, each half of atetrameric DVD-Ig molecule is reminiscent of one half of an IgG moleculeand comprises a heavy chain DVD polypeptide and a light chain DVDpolypeptide, but unlike a pair of heavy and light chains of an IgGmolecule that provides a single antigen binding domain, a pair of heavyand light chains of a DVD-Ig provide two or more antigen binding sites.

Each antigen binding site of a DVD-Ig binding protein may be derivedfrom a donor (“parental”) monoclonal antibody and thus comprises a heavychain variable domain (VH) and a light chain variable domain (VL) with atotal of six CDRs involved in antigen binding per antigen binding site.Accordingly, a DVD-Ig binding protein that binds two different epitopes(i.e., two different epitopes of two different antigen molecules or twodifferent epitopes of the same antigen molecule) comprises an antigenbinding site derived from a first parental monoclonal antibody and anantigen binding site of a second parental monoclonal antibody.

A description of the design, expression, and characterization of DVD-Igbinding molecules is provided in PCT Publication No. WO 2007/024715,U.S. Pat. No. 7,612,181, and Wu et al., Nature Biotech., 25: 1290-1297(2007). A preferred example of such DVD-Ig molecules comprises a heavychain that comprises the structural formula VD1-(X1)n-VD2-C-(X2)n,wherein VD1 is a first heavy chain variable domain, VD2 is a secondheavy chain variable domain, C is a heavy chain constant domain, X1 is alinker with the proviso that it is not CH1, X2 is an Fc region, and n is0 or 1, but preferably 1; and a light chain that comprises thestructural formula VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first lightchain variable domain, VD2 is a second light chain variable domain, C isa light chain constant domain, X1 is a linker with the proviso that itis not CH1, and X2 does not comprise an Fc region; and n is 0 or 1, butpreferably 1. Such a DVD-Ig may comprise two such heavy chains and twosuch light chains, wherein each chain comprises variable domains linkedin tandem without an intervening constant region between variableregions, wherein a heavy chain and a light chain associate to formtandem functional antigen binding sites, and a pair of heavy and lightchains may associate with another pair of heavy and light chains to forma tetrameric binding protein with four functional antigen binding sites.In another example, a DVD-Ig molecule may comprise heavy and lightchains that each comprise three variable domains (VD1, VD2, VD3) linkedin tandem without an intervening constant region between variabledomains, wherein a pair of heavy and light chains may associate to formthree antigen binding sites, and wherein a pair of heavy and lightchains may associate with another pair of heavy and light chains to forma tetrameric binding protein with six antigen binding sites.

In a preferred embodiment, a DVD-Ig binding protein according to theinvention not only binds the same target molecules bound by its parentalmonoclonal antibodies, but also possesses one or more desirableproperties of one or more of its parental monoclonal antibodies.Preferably, such an additional property is an antibody parameter of oneor more of the parental monoclonal antibodies. Antibody parameters thatmay be contributed to a DVD-Ig binding protein from one or more of itsparental monoclonal antibodies include, but are not limited to, antigenspecificity, antigen affinity, potency, biological function, epitoperecognition, protein stability, protein solubility, productionefficiency, immunogenicity, pharmacokinetics, bioavailability, tissuecross reactivity, and orthologous antigen binding.

A DVD-Ig binding protein binds at least one epitope of a GP73.Non-limiting examples of a DVD-Ig binding protein include a DVD-Igbinding protein that binds one or more epitopes of GP73, a DVD-Igbinding protein that binds an epitope of a human GP73 and an epitope ofa GP73 of another species (for example, mouse), and a DVD-Ig bindingprotein that binds an epitope of a human GP73 and an epitope of anothertarget molecule.

“Epitope,” or “epitopes,” or “epitopes of interest” refer to a site(s)on any molecule that is recognized and can bind to a complementarysite(s) on its specific binding partner. The molecule and specificbinding partner are part of a specific binding pair. For example, anepitope can be on a polypeptide, a protein, a hapten, a carbohydrateantigen (such as, but not limited to, glycolipids, glycoproteins orlipopolysaccharides), or a polysaccharide. Its specific binding partnercan be, but is not limited to, an antibody.

“F(ab′)2 fragment” as used herein refers to antibodies generated bypepsin digestion of whole IgG antibodies to remove most of the Fc regionwhile leaving intact some of the hinge region. F(ab′)2 fragments havetwo antigen-binding F(ab) portions linked together by disulfide bonds,and therefore are divalent with a molecular weight of about 110 kDa.Divalent antibody fragments (F(ab′)2 fragments) are smaller than wholeIgG molecules and enable a better penetration into tissue thusfacilitating better antigen recognition in immunohistochemistry. The useof F(ab′)2 fragments also avoids unspecific binding to Fc receptor onlive cells or to Protein A/G. F(ab′)2 fragments can both bind andprecipitate antigens.

“Framework” (FR) or “Framework sequence” as used herein may mean theremaining sequences of a variable region minus the CDRs. Because theexact definition of a CDR sequence can be determined by differentsystems (for example, see above), the meaning of a framework sequence issubject to correspondingly different interpretations. The six CDRs(CDR-L1, -L2, and -L3 of light chain and CDR-H1, -H2, and -H3 of heavychain) also divide the framework regions on the light chain and theheavy chain into four sub-regions (FR1, FR2, FR3, and FR4) on eachchain, in which CDR1 is positioned between FR1 and FR2, CDR2 between FR2and FR3, and CDR3 between FR3 and FR4. Without specifying the particularsub-regions as FR1, FR2, FR3, or FR4, a framework region, as referred byothers, represents the combined FRs within the variable region of asingle, naturally occurring immunoglobulin chain. As used herein, a FRrepresents one of the four sub-regions, and FRs represents two or moreof the four sub-regions constituting a framework region.

Human heavy chain and light chain FR sequences are known in the art thatcan be used as heavy chain and light chain “acceptor” frameworksequences (or simply, “acceptor” sequences) to humanize a non-humanantibody using techniques known in the art. In one embodiment, humanheavy chain and light chain acceptor sequences are selected from theframework sequences listed in publicly available databases such asV-base (hypertext transfer protocol://vbase.mrc-cpe.cam.ac.ukf) or inthe international ImMunoGeneTics® (IMGT®) information system (hypertexttransfer protocol://imgt.cines.fr/texts/IMGTrepertoire/LocusGenes/).

“Fucosylated GP73” is used herein to describe a Golgi membrane protein73 which has a fucose sugar moiety added to it.

“Functional antigen binding site” as used herein may mean a site on abinding protein (e.g. an antibody) that is capable of binding a targetantigen. The antigen binding affinity of the antigen binding site maynot be as strong as the parent binding protein, e.g., parent antibody,from which the antigen binding site is derived, but the ability to bindantigen must be measurable using any one of a variety of methods knownfor evaluating protein, e.g., antibody, binding to an antigen. Moreover,the antigen binding affinity of each of the antigen binding sites of amultivalent protein, e.g., multivalent antibody, herein need not bequantitatively the same.

“GP73” is used herein to describe Golgi protein 73, also known as Golgimembrane protein 1 (GOLM1) and Golgi phosphoprotein 2 (GOLPH2). GP73 isa protein that is encoded by the GOLM1 gene. It processes proteinsynthesized in the rough endoplasmic reticulum and assists in thetransport of protein cargo through the Golgi apparatus. GP73 is widelyexpressed in normal epithelial cells from several tissues. Upregulatedintracellular GP73 expression enhances its intracellular traffickingthrough the endosomal pathway, which provides the opportunity forendoproteolytic cleavage of GP73, resulting in the secretion oftruncated GP73. GP73 expression is upregulated in response to viralinfection but has also been found to be upregulated in hepatocytes frompatients with viral and non-viral liver disease. GP73 is overexpressedin prostate cancer and lung adenocarcinoma tissue. Fucosylatedglycosylation has been found in three quarters of secreted GP73 fromhepatocellular carcinoma patients.

“Humanized antibody” is used herein to describe an antibody thatcomprises heavy and light chain variable region sequences from anon-human species (e.g. a mouse) but in which at least a portion of theVH and/or VL sequence has been altered to be more “human-like,” i.e.,more similar to human germline variable sequences. A “humanizedantibody” is an antibody or a variant, derivative, analog, or fragmentthereof, which immunospecifically binds to an antigen of interest andwhich comprises a framework (FR) region having substantially the aminoacid sequence of a human antibody and a complementary determining region(CDR) having substantially the amino acid sequence of a non-humanantibody. As used herein, the term “substantially” in the context of aCDR refers to a CDR having an amino acid sequence at least 80%, at least85%, at least 90%, at least 95%, at least 98%, or at least 99% identicalto the amino acid sequence of a non-human antibody CDR. A humanizedantibody comprises substantially all of at least one, and typically two,variable domains (Fab, Fab′, F(ab′)2, FabC, Fv) in which all orsubstantially all of the CDR regions correspond to those of a non-humanimmunoglobulin (i.e., donor antibody) and all or substantially all ofthe framework regions are those of a human immunoglobulin consensussequence. In an embodiment, a humanized antibody also comprises at leasta portion of an immunoglobulin constant region (Fc), typically that of ahuman immunoglobulin. In some embodiments, a humanized antibody containsthe light chain as well as at least the variable domain of a heavychain. The antibody also may include the CH1, hinge, CH2, CH3, and CH4regions of the heavy chain. In some embodiments, a humanized antibodyonly contains a humanized light chain. In some embodiments, a humanizedantibody only contains a humanized heavy chain. In specific embodiments,a humanized antibody only contains a humanized variable domain of alight chain and/or humanized heavy chain.

A humanized antibody can be selected from any class of immunoglobulins,including IgM, IgG, IgD, IgA, and IgE, and any isotype, includingwithout limitation IgG1, IgG2, IgG3, and IgG4. A humanized antibody maycomprise sequences from more than one class or isotype, and particularconstant domains may be selected to optimize desired effector functionsusing techniques well-known in the art.

The framework regions and CDRs of a humanized antibody need notcorrespond precisely to the parental sequences, e.g., the donor antibodyCDR or the consensus framework may be mutagenized by substitution,insertion, and/or deletion of at least one amino acid residue so thatthe CDR or framework residue at that site does not correspond to eitherthe donor antibody or the consensus framework. In a preferredembodiment, such mutations, however, will not be extensive. Usually, atleast 80%, preferably at least 85%, more preferably at least 90%, andmost preferably at least 95% of the humanized antibody residues willcorrespond to those of the parental FR and CDR sequences. As usedherein, the term “consensus framework” refers to the framework region inthe consensus immunoglobulin sequence. As used herein, the term“consensus immunoglobulin sequence” refers to the sequence formed fromthe most frequently occurring amino acids (or nucleotides) in a familyof related immunoglobulin sequences (see, e.g., Winnaker, From Genes toClones (Verlagsgesellschaft, Weinheim, 1987)). A “consensusimmunoglobulin sequence” may thus comprise a “consensus frameworkregion(s)” and/or a “consensus CDR(s)”. In a family of immunoglobulins,each position in the consensus sequence is occupied by the amino acidoccurring most frequently at that position in the family. If two aminoacids occur equally frequently, either can be included in the consensussequence.

“Identical” or “identity,” as used herein in the context of two or morepolypeptide or polynucleotide sequences, can mean that the sequenceshave a specified percentage of residues that are the same over aspecified region. The percentage can be calculated by optimally aligningthe two sequences, comparing the two sequences over the specifiedregion, determining the number of positions at which the identicalresidue occurs in both sequences to yield the number of matchedpositions, dividing the number of matched positions by the total numberof positions in the specified region, and multiplying the result by 100to yield the percentage of sequence identity. In cases where the twosequences are of different lengths or the alignment produces one or morestaggered ends and the specified region of comparison includes only asingle sequence, the residues of the single sequence are included in thedenominator but not the numerator of the calculation.

“Isolated polynucleotide” as used herein may mean a polynucleotide (e.g.of genomic, cDNA, or synthetic origin, or a combination thereof) that,by virtue of its origin, the isolated polynucleotide is not associatedwith all or a portion of a polynucleotide with which the “isolatedpolynucleotide” is found in nature; is operably linked to apolynucleotide that it is not linked to in nature; or does not occur innature as part of a larger sequence.

“Label” and “detectable label” as used herein refer to a moiety attachedto an antibody or an analyte to render the reaction between the antibodyand the analyte detectable, and the antibody or analyte so labeled isreferred to as “detectably labeled.” A label can produce a signal thatis detectable by visual or instrumental means. Various labels includesignal-producing substances, such as chromagens, fluorescent compounds,chemiluminescent compounds, radioactive compounds, and the like.Representative examples of labels include moieties that produce light,e.g., acridinium compounds, and moieties that produce fluorescence,e.g., fluorescein. Other labels are described herein. In this regard,the moiety, itself, may not be detectable but may become detectable uponreaction with yet another moiety. Use of the term “detectably labeled”is intended to encompass such labeling.

Any suitable detectable label as is known in the art can be used. Forexample, the detectable label can be a radioactive label (such as 3H,14C, 32P, 33P, 35S, 90Y, 99Tc, 111In, 125I, 131I, 177Lu, 166Ho, and153Sm), an enzymatic label (such as horseradish peroxidase, alkalineperoxidase, glucose 6-phosphate dehydrogenase, and the like), achemiluminescent label (such as acridinium esters, thioesters, orsulfonamides; luminol, isoluminol, phenanthridinium esters, and thelike), a fluorescent label (such as fluorescein (e.g., 5-fluorescein,6-carboxyfluorescein, 3′6-carboxyfluorescein, 5(6)-carboxyfluorescein,6-hexachloro-fluorescein, 6-tetrachlorofluorescein, fluoresceinisothiocyanate, and the like)), rhodamine, phycobiliproteins,R-phycoerythrin, quantum dots (e.g., zinc sulfide-capped cadmiumselenide), a thermometric label, or an immuno-polymerase chain reactionlabel. An introduction to labels, labeling procedures and detection oflabels is found in Polak and Van Noorden, Introduction toImmunocytochemistry, 2nd ed., Springer Verlag, N.Y. (1997), and inHaugland, Handbook of Fluorescent Probes and Research Chemicals (1996),which is a combined handbook and catalogue published by MolecularProbes, Inc., Eugene, Oreg. A fluorescent label can be used in FPIA(see, e.g., U.S. Pat. Nos. 5,593,896, 5,573,904, 5,496,925, 5,359,093,and 5,352,803, which are hereby incorporated by reference in theirentireties). An acridinium compound can be used as a detectable label ina homogeneous chemiluminescent assay (see, e.g., Adamczyk et al.,Bioorg. Med. Chem. Lett. 16: 1324-1328 (2006); Adamczyk et al., Bioorg.Med. Chem. Lett. 4: 2313-2317 (2004); Adamczyk et al., Biorg. Med. Chem.Lett. 14: 3917-3921 (2004); and Adamczyk et al., Org. Lett. 5: 3779-3782(2003)).

In one aspect, the acridinium compound is an acridinium-9-carboxamide.Methods for preparing acridinium 9-carboxamides are described inMattingly, J. Biolumin. Chemilumin. 6: 107-114 (1991); Adamczyk et al.,J. Org. Chem. 63: 5636-5639 (1998); Adamczyk et al., Tetrahedron 55:10899-10914 (1999); Adamczyk et al., Org. Lett. 1: 779-781 (1999);Adamczyk et al., Bioconjugate Chem. 11: 714-724 (2000); Mattingly etal., In Luminescence Biotechnology: Instruments and Applications; Dyke,K. V. Ed.; CRC Press: Boca Raton, pp. 77-105 (2002); Adamczyk et al.,Org. Lett. 5: 3779-3782 (2003); and U.S. Pat. Nos. 5,468,646, 5,543,524and 5,783,699 (each of which is incorporated herein by reference in itsentirety for its teachings regarding same).

Another example of an acridinium compound is an acridinium-9-carboxylatearyl ester. An example of an acridinium-9-carboxylate aryl ester offormula II is 10-methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate(available from Cayman Chemical, Ann Arbor, Mich.). Methods forpreparing acridinium 9-carboxylate aryl esters are described in McCapraet al., Photochem. Photobiol. 4: 1111-21 (1965); Razavi et al.,Luminescence 15: 245-249 (2000); Razavi et al., Luminescence 15: 239-244(2000); and U.S. Pat. No. 5,241,070 (each of which is incorporatedherein by reference in its entirety for its teachings regarding same).Such acridinium-9-carboxylate aryl esters are efficient chemiluminescentindicators for hydrogen peroxide produced in the oxidation of an analyteby at least one oxidase in terms of the intensity of the signal and/orthe rapidity of the signal. The course of the chemiluminescent emissionfor the acridinium-9-carboxylate aryl ester is completed rapidly, i.e.,in under 1 second, while the acridinium-9-carboxamide chemiluminescentemission extends over 2 seconds. Acridinium-9-carboxylate aryl ester,however, loses its chemiluminescent properties in the presence ofprotein. Therefore, its use requires the absence of protein duringsignal generation and detection. Methods for separating or removingproteins in the sample are well-known to those skilled in the art andinclude, but are not limited to, ultrafiltration, extraction,precipitation, dialysis, chromatography, and/or digestion (see, e.g.,Wells, High Throughput Bioanalytical Sample Preparation. Methods andAutomation Strategies, Elsevier (2003)). The amount of protein removedor separated from the test sample can be about 40%, about 45%, about50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%,about 85%, about 90%, or about 95%. Further details regardingacridinium-9-carboxylate aryl ester and its use are set forth in U.S.patent application Ser. No. 11/697,835, filed Apr. 9, 2007.Acridinium-9-carboxylate aryl esters can be dissolved in any suitablesolvent, such as degassed anhydrous N,N-dimethylformamide (DMF) oraqueous sodium cholate.

“Linking sequence” or “linking peptide sequence” refers to a natural orartificial polypeptide sequence that is connected to one or morepolypeptide sequences of interest (e.g., full-length, fragments, etc.).The term “connected” refers to the joining of the linking sequence tothe polypeptide sequence of interest. Such polypeptide sequences arepreferably joined by one or more peptide bonds. Linking sequences canhave a length of from about 4 to about 50 amino acids. Preferably, thelength of the linking sequence is from about 6 to about 30 amino acids.Natural linking sequences can be modified by amino acid substitutions,additions, or deletions to create artificial linking sequences.Exemplary linking sequences include, but are not limited to: (i)Histidine (His) tags, such as a 6×His tag, which has an amino acidsequence of HHHHHH (SEQ ID NO:105), are useful as linking sequences tofacilitate the isolation and purification of polypeptides and antibodiesof interest; (ii) Enterokinase cleavage sites, like His tags, are usedin the isolation and purification of proteins and antibodies ofinterest. Often, enterokinase cleavage sites are used together with Histags in the isolation and purification of proteins and antibodies ofinterest. Various enterokinase cleavage sites are known in the art.Examples of enterokinase cleavage sites include, but are not limited to,the amino acid sequence of DDDDK (SEQ ID NO:106) and derivatives thereof(e.g., ADDDDK (SEQ ID NO:107), etc.); (iii) Miscellaneous sequences canbe used to link or connect the light and/or heavy chain variable regionsof single chain variable region fragments. Examples of other linkingsequences can be found in Bird et al., Science 242: 423-426 (1988);Huston et al., PNAS USA 85: 5879-5883 (1988); and McCafferty et al.,Nature 348: 552-554 (1990). Linking sequences also can be modified foradditional functions, such as attachment of drugs or attachment to solidsupports. In the context of the present disclosure, the monoclonalantibody, for example, can contain a linking sequence, such as a Histag, an enterokinase cleavage site, or both.

“Liver cancer” as used herein refers to cancer that originates in theliver. Liver cancer includes hepatocellular carcinoma (HCC) andfibrolamellar carcinoma. In most cases, the cause of liver cancer isusually scarring of the liver (i.e., cirrhosis).

“Liver cirrhosis” as used herein refers to the consequence of chronicliver disease characterized by replacement of liver tissue by fibrosis,scar tissue, and regenerative nodules (lumps that occur because of aprocess in which damaged tissue is regenerated, leading to loss of liverfunction. The architectural organization of the functional units of theliver become so disrupted that blood flow through the liver and liverfunction become disrupted. Cirrhosis is most commonly caused byalcoholism, hepatitis B and C, and fatty liver disease, but has manyother possible causes. Some cases are idiopathic (i.e., of unknowncause). Once cirrhosis has developed, the serious complications of liverdisease may occur including portal hypertension, liver failure, andliver cancer. The risk of liver cancer is greatly increased oncecirrhosis develops and cirrhosis should be considered to be apre-malignant condition. Cirrhosis may be caused by alcohol abuse,autoimmune diseases of the liver, Hepatitis B or C virus infection,inflammation of the liver that is long-term (chronic), and iron overloadin the body (hemochromatosis). Patients with hepatitis B or C are atrisk for liver cancer, even if they have not developed cirrhosis.

“Liver disease” as used herein refers to damage to or disease of theliver. Symptoms of liver dysfunction include both physical signs and avariety of symptoms related to digestive problems, blood sugar problems,immune disorders, abnormal absorption of fats, and metabolism problems.Liver disease includes liver fibrosis, liver cirrhosis, and livercancer. All chronic liver diseases can lead to liver fibrosis. Chronicliver disease may be caused by chronic viral hepatitis B and alcoholicliver disease.

“Liver fibrosis” as used herein refers to an excessive accumulation ofextracellular matrix proteins including collagen that occurs in mosttypes of chronic liver diseases. Liver fibrosis is the scarring processthat represents the liver's response to injury or illness. Liverfibrosis may be cause by infections due hepatitis B and C, parasites,excessive alcohol use and exposure to toxic chemicals, includingpharmaceutical drugs and blocked bile ducts. Advanced liver fibrosisresults in cirrhosis, liver failure, and portal hypertension and oftenrequires liver transplantation.

“Monoclonal antibody” as used herein refers to an antibody obtained froma population of substantially homogeneous antibodies, i.e., theindividual antibodies comprising the population are identical except forpossible naturally occurring mutations that may be present in minoramounts. Monoclonal antibodies are highly specific, being directedagainst a single antigen. Furthermore, in contrast to polyclonalantibody preparations that typically include different antibodiesdirected against different determinants (epitopes), each monoclonalantibody is directed against a single determinant on the antigen. Themonoclonal antibodies herein specifically include “chimeric” antibodiesin which a portion of the heavy and/or light chain is identical with orhomologous to corresponding sequences in antibodies derived from aparticular species or belonging to a particular antibody class orsubclass, while the remainder of the chain(s) is identical with orhomologous to corresponding sequences in antibodies derived from anotherspecies or belonging to another antibody class or subclass, as well asfragments of such antibodies, so long as they exhibit the desiredbiological.

“Multivalent binding protein” is used herein to refer to a bindingprotein comprising two or more antigen binding sites (also referred toherein as “antigen binding domains”). A multivalent binding protein ispreferably engineered to have three or more antigen binding sites, andis generally not a naturally occurring antibody. The term “multispecificbinding protein” refers to a binding protein that can bind two or morerelated or unrelated targets, including a binding protein capable ofbinding two or more different epitopes of the same target molecule.

“Predetermined cutoff” and “predetermined level” as used herein refer toan assay cutoff value that is used to assess diagnostic, prognostic, ortherapeutic efficacy results by comparing the assay results against thepredetermined cutoff/level, where the predetermined cutoff/level alreadyhas been linked or associated with various clinical parameters (e.g.,presence of disease, stage of disease, severity of disease, progression,non-progression, or improvement of disease, etc.). The disclosureprovides exemplary predetermined levels. However, it is well-known thatcutoff values may vary depending on the nature of the immunoassay (e.g.,antibodies employed, reaction conditions, sample purity, etc.). Itfurther is well within the ordinary skill of one in the art to adapt thedisclosure herein for other immunoassays to obtain immunoassay-specificcutoff values for those other immunoassays based on the descriptionprovided by this disclosure. Whereas the precise value of thepredetermined cutoff/level may vary between assays, the correlations asdescribed herein should be generally applicable.

“Pretreatment reagent,” e.g., lysis, precipitation and/or solubilizationreagent, as used in a diagnostic assay as described herein is one thatlyses any cells and/or solubilizes any analyte that is/are present in atest sample. Pretreatment is not necessary for all samples, as describedfurther herein. Among other things, solubilizing the analyte (i.e.,GP73, fragments of GP73, variants of GP73 or any combinations thereof)entails release of the analyte from any endogenous binding proteinspresent in the sample. A pretreatment reagent may be homogeneous (notrequiring a separation step) or heterogeneous (requiring a separationstep). With use of a heterogeneous pretreatment reagent, there isremoval of any precipitated analyte binding proteins from the testsample prior to proceeding to the next step of the assay. Thepretreatment reagent optionally can comprise: (a) one or more solventsand salt, (b) one or more solvents, salt and detergent, (c) detergent,(d) detergent and salt, or (e) any reagent or combination of reagentsappropriate for cell lysis and/or solubilization of analyte.

“Quality control reagents” in the context of immunoassays and kitsdescribed herein, include, but are not limited to, calibrators,controls, and sensitivity panels. A “calibrator” or “standard” typicallyis used (e.g., one or more, such as a plurality) in order to establishcalibration (standard) curves for interpolation of the concentration ofan analyte, such as an antibody or an analyte. Alternatively, a singlecalibrator, which is near a predetermined positive/negative cutoff, canbe used. Multiple calibrators (i.e., more than one calibrator or avarying amount of calibrator(s)) can be used in conjunction to comprisea “sensitivity panel.”

A “receiver operating characteristic” curve or “ROC” curve refers to agraphical plot that illustrates the performance of a binary classifiersystem as its discrimination threshold is varied. It is created byplotting the fraction of true positives out of the positives (TPR=truepositive rate) vs. the fraction of false positives out of the negatives(FPR=false positive rate), at various threshold settings. TPR is alsoknown as sensitivity, and FPR is one minus the specificity or truenegative rate.

“Recombinant antibody” and “recombinant antibodies” refer to antibodiesprepared by one or more steps, including cloning nucleic acid sequencesencoding all or a part of one or more monoclonal antibodies into anappropriate expression vector by recombinant techniques and subsequentlyexpressing the antibody in an appropriate host cell. The terms include,but are not limited to, recombinantly produced monoclonal antibodies,chimeric antibodies, humanized antibodies (fully or partiallyhumanized), multi-specific or multi-valent structures formed fromantibody fragments, bifunctional antibodies, heteroconjugate Abs,DVD-Ig®s, and other antibodies as described in (i) herein.(Dual-variable domain immunoglobulins and methods for making them aredescribed in Wu, C., et al., Nature Biotechnology, 25:1290-1297 (2007)).The term “bifunctional antibody,” as used herein, refers to an antibodythat comprises a first arm having a specificity for one antigenic siteand a second arm having a specificity for a different antigenic site,i.e., the bifunctional antibodies have a dual specificity.

“Risk assessment,” “risk classification,” “risk identification,” or“risk stratification” of subjects (e.g., patients) as used herein refersto the evaluation of factors including biomarkers, to predict the riskof occurrence of future events including disease onset or diseaseprogression, so that treatment decisions regarding the subject may bemade on a more informed basis.

“Sample,” “test sample,” “specimen,” “sample from a subject,” and“patient sample” as used herein may be used interchangeable and may be asample of blood, tissue, urine, serum, plasma, amniotic fluid,cerebrospinal fluid, placental cells or tissue, endothelial cells,leukocytes, or monocytes. The sample can be used directly as obtainedfrom a patient or can be pre-treated, such as by filtration,distillation, extraction, concentration, centrifugation, inactivation ofinterfering components, addition of reagents, and the like, to modifythe character of the sample in some manner as discussed herein orotherwise as is known in the art.

Any cell type, tissue, or bodily fluid may be utilized to obtain asample. Such cell types, tissues, and fluid may include sections oftissues such as biopsy and autopsy samples, frozen sections taken forhistologic purposes, blood (such as whole blood), plasma, serum, sputum,stool, tears, mucus, saliva, bronchoalveolar lavage (BAL) fluid, hair,skin, red blood cells, platelets, interstitial fluid, ocular lens fluid,cerebral spinal fluid, sweat, nasal fluid, synovial fluid, menses,amniotic fluid, semen, etc. Cell types and tissues may also includelymph fluid, ascetic fluid, gynecological fluid, urine, peritonealfluid, cerebrospinal fluid, a fluid collected by vaginal rinsing, or afluid collected by vaginal flushing. A tissue or cell type may beprovided by removing a sample of cells from an animal, but can also beaccomplished by using previously isolated cells (e.g., isolated byanother person, at another time, and/or for another purpose). Archivaltissues, such as those having treatment or outcome history, may also beused. Protein or nucleotide isolation and/or purification may not benecessary.

“Series of calibrating compositions” refers to a plurality ofcompositions comprising a known concentration of GP73, wherein each ofthe compositions differs from the other compositions in the series bythe concentration of GP73.

“Solid phase” refers to any material that is insoluble, or can be madeinsoluble by a subsequent reaction. The solid phase can be chosen forits intrinsic ability to attract and immobilize a capture agent.Alternatively, the solid phase can have affixed thereto a linking agentthat has the ability to attract and immobilize the capture agent. Forexample, the linking agent can include a charged substance that isoppositely charged with respect to the capture agent itself or to acharged substance conjugated to the capture agent. In general, thelinking agent can be any binding partner (preferably specific) that isimmobilized on (attached to) the solid phase and that has the ability toimmobilize the capture agent through a binding reaction. The linkingagent enables the indirect binding of the capture agent to a solid phasematerial before the performance of the assay or during the performanceof the assay. For examples, the solid phase can be plastic, derivatizedplastic, magnetic, or non-magnetic metal, glass or silicon, including,for example, a test tube, microtiter well, sheet, bead, microparticle,chip, and other configurations known to those of ordinary skill in theart.

“Specific binding” or “specifically binding” as used herein may refer tothe interaction of an antibody, a protein, or a peptide with a secondchemical species, wherein the interaction is dependent upon the presenceof a particular structure (e.g., an antigenic determinant or epitope) onthe chemical species; for example, an antibody recognizes and binds to aspecific protein structure rather than to proteins generally. If anantibody is specific for epitope “A”, the presence of a moleculecontaining epitope A (or free, unlabeled A), in a reaction containinglabeled “A” and the antibody, will reduce the amount of labeled A boundto the antibody.

“Specific binding partner” is a member of a specific binding pair. Aspecific binding pair comprises two different molecules, whichspecifically bind to each other through chemical or physical means.Therefore, in addition to antigen and antibody specific binding pairs ofcommon immunoassays, other specific binding pairs can include biotin andavidin (or streptavidin), carbohydrates and lectins, complementarynucleotide sequences, effector and receptor molecules, cofactors andenzymes, enzymes and enzyme inhibitors, and the like. Furthermore,specific binding pairs can include members that are analogs of theoriginal specific binding members, for example, an analyte-analog.Immunoreactive specific binding members include antigens, antigenfragments, and antibodies, including monoclonal and polyclonalantibodies as well as complexes and fragments thereof, whether isolatedor recombinantly produced.

“Subject” and “patient” as used herein interchangeably refers to anyvertebrate, including, but not limited to, a mammal (e.g., cow, pig,camel, llama, horse, goat, rabbit, sheep, hamsters, guinea pig, cat,dog, rat, and mouse, a non-human primate (for example, a monkey, such asa cynomolgous or rhesus monkey, chimpanzee, etc) and a human). In someembodiments, the subject may be a human or a non-human. The subject orpatient may be undergoing other forms of treatment.

“Treat”, “treating” or “treatment” are each used interchangeably hereinto describe reversing, alleviating, or inhibiting the progress of adisease, or one or more symptoms of such disease, to which such termapplies. Depending on the condition of the subject, the term also refersto preventing a disease, and includes preventing the onset of a disease,or preventing the symptoms associated with a disease. A treatment may beeither performed in an acute or chronic way. The term also refers toreducing the severity of a disease or symptoms associated with suchdisease prior to affliction with the disease. Such prevention orreduction of the severity of a disease prior to affliction refers toadministration of an antibody or pharmaceutical composition of thepresent invention to a subject that is not at the time of administrationafflicted with the disease. “Preventing” also refers to preventing therecurrence of a disease or of one or more symptoms associated with suchdisease. “Treatment” and “therapeutically,” refer to the act oftreating, as “treating” is defined above.

“Variant” is used herein to describe a peptide or polypeptide thatdiffers in amino acid sequence by the insertion, deletion, orconservative substitution of amino acids, but retain at least onebiological activity. Representative examples of “biological activity”include the ability to be bound by a specific antibody or to promote animmune response. Variant is also used herein to describe a protein withan amino acid sequence that is substantially identical to a referencedprotein with an amino acid sequence that retains at least one biologicalactivity. A conservative substitution of an amino acid, i.e., replacingan amino acid with a different amino acid of similar properties (e.g.,hydrophilicity, degree, and distribution of charged regions) isrecognized in the art as typically involving a minor change. These minorchanges can be identified, in part, by considering the hydropathic indexof amino acids, as understood in the art. Kyte et al., J. Mol. Biol.157:105-132 (1982). The hydropathic index of an amino acid is based on aconsideration of its hydrophobicity and charge. It is known in the artthat amino acids of similar hydropathic indexes can be substituted andstill retain protein function. In one aspect, amino acids havinghydropathic indexes of ±2 are substituted. The hydrophilicity of aminoacids can also be used to reveal substitutions that would result inproteins retaining biological function. A consideration of thehydrophilicity of amino acids in the context of a peptide permitscalculation of the greatest local average hydrophilicity of thatpeptide, a useful measure that has been reported to correlate well withantigenicity and immunogenicity. U.S. Pat. No. 4,554,101, incorporatedfully herein by reference. Substitution of amino acids having similarhydrophilicity values can result in peptides retaining biologicalactivity, for example immunogenicity, as is understood in the art.Substitutions may be performed with amino acids having hydrophilicityvalues within ±2 of each other. Both the hydrophobicity index and thehydrophilicity value of amino acids are influenced by the particularside chain of that amino acid. Consistent with that observation, aminoacid substitutions that are compatible with biological function areunderstood to depend on the relative similarity of the amino acids, andparticularly the side chains of those amino acids, as revealed by thehydrophobicity, hydrophilicity, charge, size, and other properties.“Variant” also can be used to refer to an antigenically reactivefragment of an anti-GP73 antibody that differs from the correspondingfragment of anti-GP73 antibody in amino acid sequence but is stillantigenically reactive and can compete with the corresponding fragmentof anti-GP73 antibody for binding with GP73. “Variant” also can be usedto describe a polypeptide or a fragment thereof that has beendifferentially processed, such as by proteolysis, phosphorylation, orother post-translational modification, yet retains its antigenreactivity.

“Vector” is used herein to describe a nucleic acid molecule that cantransport another nucleic acid to which it has been linked. One type ofvector is a “plasmid”, which refers to a circular double-stranded DNAloop into which additional DNA segments may be ligated. Another type ofvector is a viral vector, wherein additional DNA segments may be ligatedinto the viral genome. Certain vectors can replicate autonomously in ahost cell into which they are introduced (e.g., bacterial vectors havinga bacterial origin of replication and episomal mammalian vectors). Othervectors (e.g., non-episomal mammalian vectors) can be integrated intothe genome of a host cell upon introduction into the host cell, andthereby are replicated along with the host genome. Moreover, certainvectors are capable of directing the expression of genes to which theyare operatively linked. Such vectors are referred to herein as“recombinant expression vectors” (or simply, “expression vectors”). Ingeneral, expression vectors of utility in recombinant DNA techniques areoften in the form of plasmids. “Plasmid” and “vector” may be usedinterchangeably as the plasmid is the most commonly used form of vector.However, other forms of expression vectors, such as viral vectors (e.g.,replication defective retroviruses, adenoviruses and adeno-associatedviruses), which serve equivalent functions, can be used. In this regard,RNA versions of vectors (including RNA viral vectors) may also find usein the context of the present disclosure.

Unless otherwise defined herein, scientific and technical terms used inconnection with the present disclosure shall have the meanings that arecommonly understood by those of ordinary skill in the art. For example,any nomenclatures used in connection with, and techniques of, cell andtissue culture, molecular biology, immunology, microbiology, geneticsand protein and nucleic acid chemistry and hybridization describedherein are those that are well known and commonly used in the art. Themeaning and scope of the terms should be clear; in the event, however ofany latent ambiguity, definitions provided herein take precedent overany dictionary or extrinsic definition. Further, unless otherwiserequired by context, singular terms shall include pluralities and pluralterms shall include the singular.

2. GP73 ANTIBODIES

Provided herein are antibodies for use in methods of detecting andtreating diseases, such as liver disease and/or cancer. An isolatedantibody that specifically binds to Golgi protein 73 (“GP73”) (orfragments thereof) is provided herein, and referred to as “GP73antibody”.

a. Golgi Protein 73 (GP73)

Golgi protein 73 (GP73), also known as “Golgi membrane protein 1”,“Golgi phosphoprotein 2” and “Golgi membrane protein GP73”, is a proteinthat processes protein synthesized in the rough endoplasmic reticulumand assists in the transport of protein cargo through the Golgiapparatus. Human GP73 is a 400 amino acid protein encoded by the GOLM1gene. GP73 is widely expressed in normal epithelial cells from severaltissues. Upregulated intracellular GP73 expression enhances itsintracellular trafficking through the endosomal pathway, which providesthe opportunity for endoproteolytic cleavage of GP73, resulting in thesecretion of truncated GP73. GP73 expression is upregulated in responseto viral infection but has also been found to be upregulated inhepatocytes from patients with viral and non-viral liver disease. GP73is overexpressed in prostate cancer and lung adenocarcinoma tissue.Human GP73 may have the following amino acid sequence:

MGLGNGRRSMKSPPLVLAALVACIIVLGFNYWIASSRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQ REKRNHTL (SEQ ID NO:97; GenBank Accession NO: AAF44663).

The human GP73 may be a fragment or variant of SEQ ID NO:97. Thefragment of GP73 may be between 5 and 400 amino acids, between 10 and400 amino acids, between 50 and 400 amino acids, between 60 and 400amino acids, between 65 and 400 amino acids, between 100 and 400 aminoacids, between 150 and 400 amino acids, between 100 and 300 amino acids,or between 200 and 300 amino acids in length. The fragment may comprisea contiguous number of amino acids from SEQ ID NO:97.

The fragment of human GP73 may have the following amino acid sequence:

ELKKNEELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHT L which correspond(SEQ ID NO: 100), to amino acids 63-400 of SEQ ID NO: 97.

b. GP73-Recognizing Antibody

The antibody is an antibody that binds to GP73, a fragment thereof, anepitope of GP73, or a variant thereof. The antibody may be a fragment ofthe anti-GP73 antibody or a variant or a derivative thereof. Theantibody may be a polyclonal or monoclonal antibody. The antibody may bea chimeric antibody, a single chain antibody, an affinity maturedantibody, a human antibody, a humanized antibody, a fully human antibodyor an antibody fragment, such as a Fab fragment, or a mixture thereof.Antibody fragments or derivatives may comprise F(ab′)₂, Fv or scFvfragments. The antibody derivatives can be produced by peptidomimetics.Further, techniques described for the production of single chainantibodies can be adapted to produce single chain antibodies.

The anti-GP73 antibodies may be a chimeric anti-GP73 or humanizedanti-GP73 antibody. In one embodiment, both the humanized antibody andchimeric antibody are monovalent. In one embodiment, both the humanizedantibody and chimeric antibody comprise a single Fab region linked to anFc region.

Human antibodies may be derived from phage-display technology or fromtransgenic mice that express human immunoglobulin genes. The humanantibody may be generated as a result of a human in vivo immune responseand isolated. See, for example, Funaro et al., BMC Biotechnology,2008(8):85. Therefore, the antibody may be a product of the human andnot animal repertoire. Because it is of human origin, the risks ofreactivity against self-antigens may be minimized. Alternatively,standard yeast display libraries and display technologies may be used toselect and isolate human anti-GP73 antibodies. For example, libraries ofnaïve human single chain variable fragments (scFv) may be used to selecthuman anti-GP73 antibodies. Transgenic animals may be used to expresshuman antibodies.

Humanized antibodies may be antibody molecules from non-human speciesantibody that binds the desired antigen having one or morecomplementarity determining regions (CDRs) from the non-human speciesand framework regions from a human immunoglobulin molecule.

The antibody is distinguishable from known antibodies in that itpossesses different biological function(s) than those known in the art.

(1) Epitope

The antibody may immunospecifically bind to GP73 (SEQ ID NO:97), SEQ IDNO:100, a fragment thereof, or a variant thereof. The antibody mayimmunospecifically recognize and bind to an epitope peptide of SEQ IDNO:101 (amino acids 307-339 of SEQ ID NO:97), SEQ ID NO: 102 (aminoacids 276-287 of SEQ ID NO:97), SEQ ID NO: 103 (amino acids 344-363 ofSEQ ID NO:97), or SEQ ID NO: 104 (amino acids 63-96 of SEQ ID NO:97).The antibody may immunospecifically recognize and bind at least threeamino acids, at least four amino acids, at least five amino acids, atleast six amino acids, at least seven amino acids, at least eight aminoacids, at least nine amino acids, or at least ten amino acids within theepitope peptides of SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 103, orSEQ ID NO: 104. The antibody may immunospecifically recognize and bindto an epitope that has at least three contiguous amino acids, at leastfour contiguous amino acids, at least five contiguous amino acids, atleast six contiguous amino acids, at least seven contiguous amino acids,at least eight contiguous amino acids, at least nine contiguous aminoacids, or at least ten contiguous amino acids of the epitope peptides ofSEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 103, or SEQ ID NO: 104.

(2) Antibody Binding Characteristics

The antibody may immunospecifically bind to GP73 (SEQ ID NO:97), SEQ IDNO:100, a fragment thereof, or a variant thereof and has a K_(D) of atleast 1.5×10⁻¹² M, of at least 1.6×10⁻¹² M, of at least 1.7×10⁻¹² M, ofat least 1.8×10⁻¹² M, of at least 1.9×10⁻¹²M, of at least 2.0×10⁻¹² M,of at least 2.1×10⁻¹² M, of at least 2.2×10⁻¹² M, of at least 2.3×10⁻¹²M, of at least 2.4×10⁻¹² M, of at least 2.5×10⁻¹² M, of at least5.0×10⁻¹² M, of at least 10.0×10⁻¹² M, of at least 15×10⁻¹² M, of atleast 50×10⁻¹² M, of at least 100×10⁻¹² M, of at least 500×10⁻¹² M, ofat least 1000×10⁻¹² M, of at least 2000×10⁻¹² M, of at least 3000×10⁻¹²M, of at least 3500×10⁻¹² M, or has a K_(d) ranging from about 1.5×10⁻¹²M to about 3500×10⁻¹² M, from about 10.0×10⁻¹² M to about 3500×10⁻¹² M,from about 25.0×10⁻¹² M to about 3500×10⁻¹² M, from about 50.0×10⁻¹² Mto about 3500×10⁻¹² M, from about 100.0×10⁻¹² M to about 3500×10⁻¹² M,from about 150.0×10⁻¹² M to about 3500×10⁻¹² M, from about 200×10⁻¹² Mto about 3500×10⁻¹² M, from about 500×10⁻¹² M to about 3500×10⁻¹² M,from about 1000×10⁻¹² M to about 3500×10⁻¹² M, from about 1.5×10⁻¹² M toabout 3000×10⁻¹² M, from about 10.0×10⁻¹² M to about 3000×10⁻¹² M, fromabout 25.0×10⁻¹² M to about 3000×10⁻¹² M, from about 50.0×10⁻¹² M toabout 3000×10⁻¹² M, from about 1.5×10⁻¹² M to about 1000×10⁻¹² M, fromabout 25.0×10⁻¹² M to about 1000×10⁻¹² M, from about 50.0×10⁻¹² M toabout 1000×10⁻¹² M, from about 100.0×10⁻¹² M to about 1000×10⁻¹² M, fromabout 1.5×10⁻¹² M to about 500×10⁻¹² M, from about 50×10⁻¹² M to about500×10⁻¹² M, from about 100×10⁻¹² M to about 500×10⁻¹² M, from about1.5×10⁻¹² M to about 100×10⁻¹² M, from about 25.0×10⁻¹² M to about100×10⁻¹² M, from about 50.0×10⁻¹² M to about 100×10⁻¹² M, from about1.5×10⁻¹² M to about 50×10⁻¹² M, from about 10.0×10⁻¹² M to about50.0×10⁻¹² M, or from about from about 25.0×10⁻¹² M to about 50×10⁻¹² M.The fragment may be SEQ ID NO:97 or SEQ ID NO:100.

The binding of the antibody to GP73 may be sensitive or insensitive tothe presence or absence of a fucose sugar moiety on the GP73 molecule.An antibody that is sensitive to the presence or absence of a fucosesugar moiety on the GP73 molecule means that the antibody's bindingaffinity to GP73 changes depending on whether a fucose sugar moiety ispresent or absent on the GP73 molecule. For example, an antibody whosebinding is sensitive to the presence or absence of a fucose sugar moietyon the GP73 molecule may have lower binding affinity to GP73 if a fucosesugar moiety is present. Alternatively, an antibody whose binding issensitive to the presence or absence of a fucose sugar moiety on theGP73 molecule may have lower binding affinity to GP73 if a fucose sugarmoiety is absent. An antibody that is insensitive to the presence orabsence of a fucose sugar moiety on the GP73 molecule means that theantibody's binding affinity to GP73 does not change if a fucose sugarmoiety is present or absent on the GP73 molecule.

(3) Antibody Structure

(a) Heavy Chain and Light Chain CDRs

The antibody may immunospecifically bind to GP73 (SEQ ID NO:97), SEQ IDNO:100, a fragment thereof, or a variant thereof and comprise a variableheavy chain (VH) and/or variable light chain (VL) shown in Table 1. Theantibody may immunospecifically bind to GP73, a fragment thereof, or avariant thereof, and comprises one or more of the heavy chain or lightchain CDR sequences also shown in Table 1. The light chain of theantibody may be a kappa chain or a lambda chain. For example, see Table1.

TABLE 1 SEQ ID PROTEIN REGION NO. SEQUENCE 1B-3440 (VH) 1EVQLVETGGGLVQPKGSLKLSCAASGFTFNTNAMNWVRQAPGKGLEWVARIRTKRYNYTTFYADSVKDRFTISRDDSQSMLFLQMNNLKTEDTAMYYCVTGGTGTFDY WGQGTTLTVSS 1B-3440 (VH) CDR-H1 2TNAMN 1B-3440 (VH) CDR-H2 3 RIRTKRYNYTTFYADSVKD 1B-3440 (VH) CDR-H3 4GGTGTFDY 1B-3440 (VL) 5 EIVLTQSPTTMPASPGEKVTFTCSASSGISSNYLHWYQLKPGFSPKLLIYRTSNLASGVPARFSGGGSGTSYSLTIGT MEAEDVATYYCQQGFSIPLTFGAGTKLELKR1B-3440 (VL) CDR-L1 6 SASSGISSNYLH 1B-3440 (VL) CDR-L2 7 RTSNLAS 1B-3440(VL) CDR-L3 8 QQGFSIPLT 1B-4971 (VH) 9QVQLQQSGPALVKPGASVKMSCKASGYTFTNYVIHWVKQKPGQGLERIGYIWPYNDGTKFNEKFKGKATLTSDKSSSTAYMELSSLTSEDSAVYYCSSQQLAYWGQGTTL TVSS 1B-4971 (VH) CDR-H1 10 NYVIH1B-4971 (VH) CDR-H2 11 YIWPYNDGTKFNEKFKG 1B-4971 (VH) CDR-H3 12 QQLAY1B-4971 (VL) 13 DVVMTQTPLTLSVTIGQPASISCKSSQSLLYSDGKTYLIWLLQRPGQSPKRLIYLVSKLDSGVPDRFTGSGSGTDFTLRISRVEAEDLGVYYCWQGTHFPYTFGGGTKLEIKR 1B-4971 (VL) CDR-L1 14KSSQSLLYSDGKTYLI 1B-4971 (VL) CDR-L2 15 LVSKLDS 1B-4971 (VL) CDR-L3 16WQGTHFPYT 1B-3246 (VH) 17 DVKLVESGGGLVKPGGSLKLSCAASGFTFSSYTMSWVRQTPEKRLEWVATISRGGTYIYYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAIYYCTREYFSGDTYDYFDYW GQGTTLTVSS 1B-3246 (VH) CDR-H1 18SYTMS 1B-3246 (VH) CDR-H2 19 TISRGGTYIYYPDSVKG 1B-3246 (VH) CDR-H3 20EYFSGDTYDYFDY 1B-3246 (VL) 21 EIVMTQAAFANPVTLGTSVSISCRSSKSLLHSNGITYLYWYLQKPGQSPQLLIYQMSNLASGVPDRFSSSGSGTDFTLRISRVEAEDVGIYYCAQNLELYTFGGGTKLEIKR 1B-3246 (VL) CDR-L1 22RSSKSLLHSNGITYL 1B-3246 (VL) CDR-L2 23 QMSNLAS 1B-3246 (VL) CDR-L3 24AQNLELYT 1B-4863 (VH) 25 QVQLKESGPGLVAPSQSLSITCTVSGFSLTSYGVHWVRQSPGKGLEWLGVIWTGGSTNYNSALMSRLSISKDNSESQVFLKVNSLQTDDTGMYYCARDPGTDYFDYWGQGT TLTVSS 1B-4863 (VH) CDR-H1 26 SYGVH1B-4863 (VH) CDR-H2 27 VIWTGGSTNYNSALMS 1B-4863 (VH) CDR-H3 28 DPGTDYFDY1B-4863 (VL) 29 DIVMTQSHKFMSTSIGDRVSISCKASQDVSIDVSWYQQKPGQSPTLLIYSASYRYIGVPDRFTGSGSGTAFTFTISSV QAEDLAIYYCQQHFTTPLTFGAGTKLELKR1B-4863 (VL) CDR-L1 30 KASQDVSIDVS 1B-4863 (VL) CDR-L2 31 SASYRY 1B-4863(VL) CDR-L3 32 QQHFTTPLT 1A-3187 (VH) 33QGQLQQSGAELMKPGASVKISCKATGYTIRSYWIEWVKQRPGHGLEWIGEILPGSGNTNYNEKFKGTATFTADTSSNTVYLHLSSLTSEDSAVYYCANGRGSYRYHWFAYW GQGTLVTVSP 1A-3187 (VH) CDR-H1 34SYWIE 1A-3187 (VH) CDR-H2 35 EILPGSGNTNYNEKFKG 1A-3187 (VH) CDR-H3 36GRGSYRYHWFAY 1A-3187 (VL) 37 DIVLTQSPASLAVSLGQRATISCKASQSVDYDGDSYMNWYQQKPGQPPKVLIYAASNLESGIPARFSGSGSGTDFTLNIHPVEEEDAATYYCQQSNEDPYTFGGGTKLEMKR 1A-3187 (VL) CDR-L1 38KASQSVDYDGDSYMN 1A-3187 (VL) CDR-L2 39 AASNLES 1A-3187 (VL) CDR-L3 40QQSNEDPYT 1A-4246 (VH) 41 EVMLVESGGGLVKPGGSLKLSCAASGFTFSSYAMSWVRLTPEKRLEWVATISSGSSYTYYPDSVKGRFTISRDNVKSTLYLQMSSLRSEDTAMYYCARNWDGELHYYAMD YWGQGTSVTVSS 1A-4246 (VH) CDR-H1 42SYAMS 1A-4246 (VH) CDR-H2 43 TISSGSSYTYYPDSVKG 1A-4246 (VH) CDR-H3 44DGELHYYAMDY 1A-4246 (VL) 45 QIVLTQSPAIMSASLGERVTMTCTASSSVSSSYLHWYQQKPGSSPKLWVYSTSSLASGVPARFSGSGSGTSYSLTIN NMEAEDAATYFCHQYHRSPYTFGGGTKLEIKR1A-4246 (VL) CDR-L1 46 TASSSVSSSYLH 1A-4246 (VL) CDR-L2 47 STSSLAS1A-4246 (VL) CDR-L3 48 HQYHRSPYT

The antibody or variant or derivative thereof may contain one or moreamino acid sequences that are greater than 95%, 90%, 85%, 80%, 75%, 70%,65%, 60%, 55%, or 50% identical to one or more of SEQ ID NOs:1-48. Theantibody or variant or derivative thereof may be encoded by one or morenucleic acid sequences that are greater than 95%, 90%, 85%, 80%, 75%,70%, 65%, 60%, 55%, or 50% identical to one or more of SEQ ID NOs:49-96.Polypeptide identity and homology can be determined, for example, by thealgorithm described in the report: Wilbur, W. J. and Lipman, D. J. Proc.Natl. Acad. Sci. USA 80, 726-730 (1983). The herein described antibody,variant, or derivative thereof may be encoded by a nucleic acid thathybridizes under stringent conditions with the complement of one or moreof SEQ ID NOs: 17-32. The herein described antibody, variant, orderivative thereof may be encoded by a nucleic acid that hybridizesunder highly stringent conditions with the complement of one or morenucleic acids that encode one or more of SEQ ID NOs:1-48.

In one aspect, the isolated antibody specifically binds GP-73 and has aCDR-H1 having the formula: CDR-H1, X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:108),wherein: X₅ is S, N or T; X₆ is Y or N; X₇ is W, V, G, A or T; X₈ is I,V or M; and X₉ is E, H, S or N.

In another aspect, the isolated antibody specifically binds GP-73 andhas a CDR-H2 having the formula: CDR-H2,X₁₀-I-X₁₁-X₁₂-X₁₃-X₁₄-X₁₅-X₁₆-X₁₇-X₁₈-X₁₉-X₂₀-X₂₁-X₂₂-X₂₃-X₂₄-X₂₅-X₂₆-X₂₇(SEQ ID NO:109), wherein: X₁₀ is E, Y, V, T or R; X₁₁ is L, W, S or R;X₁₂ is P, S, R or T; X₁₃ is absent or is K; X₁₄ is absent or is R; X₁₅is G, T or Y; X₁₆ is S, G or N; X₁₇ is G, D, S, T or Y; X₁₈ is N, S, Y,G or T; X₁₉ is T or I; X₂₀ is N, K, Y or F; X₂₁ is Y or F; X₂₂ is N, Por A; X₂₃ is E, S or D; X₂₄ is K, A or S; X₂₅ is F, L or V; X₂₆ is K orM; and X₂₇ is G, S or D.

In yet another aspect, the isolated antibody specifically binds GP-73and has a CDR-H3 having the formula: CDR-H3,X₂₈-X₂₉-X₃₀-X₃₁-X₃₂-X₃₃-X₃₄-X₃₅-X₃₆-X₃₇-X₃₈-X₃₉-Y (SEQ ID NO:110),wherein: X₂₈ is G, Q, D, E or N; X₂₉ is R, Q, P, Y, W or G; X₃₀ is G, L,F, D or T; X₃₁ is S, T, S or G; X₃₂ is Y, D, G, E or T; X₃₃ is R, Y, D,L or F; X₃₄ is Y, F, T or H; X₃₅ is H or Y; X₃₆ is W, D or Y; X₃₇ is F,Y or A; X₃₈ is absent or is F or M; and X₃₉ is A or D.

In yet another aspect, the isolated antibody specifically binds GP-73and has a CDR-L1 having the formula: CDR-L1,X₄₀-X₄₁-S-X₄₂-X₄₃-X₄₄-X₄₅-X₄₆X₄₇-X₄₈-X₄₉-X₅₀-X₅₁-X₅₂-X₅₃-X₅₄ (SEQ IDNO:111), wherein: X₄₀ is K, T, R or S; X₄₁ is A or S; X₄₂ is Q, S or K;X₄₃ is S or G; X₄₄ is V or L; X₄₅ is D or L; X₄₆ is Y, D or H; X₄₇ isabsent or S; X₄₈ is D, V, N or I; X₄₉ is G, V or S; X₅₀ is D, K, S or I;X₅₁ is S, T, I or N; X₅₂ is Y or D; X₅₃ is M, L or V; and X₅₄ is N, I,S, H or Y.

In still yet another aspect, the isolated antibody specifically bindsGP-73 and has a CDR-L2 having the formula: CDR-L2,X₅₅-X₅₆-S-X₅₇-X₅₈-X₅₉-X₆₀ (SEQ ID NO:112), wherein: X₅₅ is A, L, S, Q orR; X₅₆ is A, V, T or M; X₅₇ is N, K, Y or S; X₅₈ is L or R; X₅₉ is E, D,Y or A; and X₆₀ is S or I.

In yet another aspect, the isolated antibody specifically binds GP-73and has CDR-L3 having the formula: CDR-L3, X₆₁-Q-X₆₂-X₆₃-X₆₄-X₆₅-P-X₆₂-T(SEQ ID NO:113), wherein: X₆₁ is Q, W, H or A; X₆₂ is S, G, H, Y or N;X₆₃ is N, T, F, H or L; X₆₄ is E, H, T, R or S; X₆₅ is D, F, T, S, L orI; and X₆₆ is Y or L.

In still yet another aspect, the isolated antibody specifically bindsGP-73 and has a CDR-H1 having the formula: X₅-X₆-X₇-X₈-X₉ (SEQ IDNO:108) a CDR-H2 having the formula:X₁₀-I-X₁₁-X₁₂-X₁₃-X₁₄-X₁₅-X₁₆X₁₇-X₁₈-X₁₉-X₂₀-X₂₁-X₂₂-X₂₃-X₂₄-X₂₅-X₂₆-X₂₇(SEQ ID NO:109), a CDR-H3 having the formula:X₂₈-X₂₉-X₃₀-X₃₁-X₃₂-X₃₃-X₃₄-X₃₅-X₃₆-X₃₇-X₃₈-X₃₉-Y (SEQ ID NO:110), aCDR-L1 having the formula:X₄₀-X₄₁-S-X₄₂-X₄₃-X₄₄-X₄₅-X₄₆X₄₇-X₄₈-X₄₉-X₅₀-X₅₁-X₅₂-X₅₃-X₅₄ (SEQ IDNO:111), a CDR-L2 having the formula: X₅₅-X₅₆-S-X₅₇-X₅₈-X₅₉-X₆₀ (SEQ IDNO:112) and a CDR-L3 having the formula: X₆₁-Q-X₆₂-X₆₃-X₆₄-X₆₅-P-X₆₂-T(SEQ ID NO:113), wherein: X₅ is S, N or T; X₆ is Y or N; X₇ is W, V, G,A or T; X₈ is I, V or M; X₉ is E, H, S or N; X₁₀ is E, Y, V, T or R; X₁₁is L, W, S or R; X₁₂ is P, S, R or T; X₁₃ is absent or is K; X₁₄ isabsent or is R; X₁₅ is G, T or Y; X₁₆ is 5, G or N; X₁₇ is G, D, S, T orY; X₁₈ is N, S, Y, G or T; X₁₉ is T or I; X₂₀ is N, K, Y or F; X₂₁ is Yor F; X₂₂ is N, P or A; X₂₃ is E, S or D; X₂₄ is K, A or S; X₂₅ is F, Lor V; X₂₆ is K or M; X₂₇ is G, S or D; X₂₈ is G, Q, D, E or N; X₂₉ is R,Q, P, Y, W or G; X₃₀ is G, L, F, D or T; X₃₁ is S, T, S or G; X₃₂ is Y,D, G, E or T; X₃₃ is R, Y, D, L or F; X₃₄ is Y, F, T or H; X₃₅ is H orY; X₃₆ is W, D or Y; X₃₇ is F, Y or A; X₃₈ is absent or is F or M; X₃₉is A or D; X₄₀ is K, T, R or S; X₄₁ is A or S; X₄₂ is Q, S or K; X₄₃ isS or G; X₄₄ is V or L; X₄₅ is D or L; X₄₆ is Y, D or H; X₄₇ is absent orS; X₄₈ is D, V, N or I; X₄₉ is G, V or S; X₅₀ is D, K, S or I; X₅₁ is S,T, I or N; X₅₂ is Y or D; X₅₃ is M, L or V; X₅₄ is N, I, S, H or Y; X₅₅is A, L, S, Q or R; X₅₆ is A, V, T or M; X₅₇ is N, K, Y or S; X₅₈ is Lor R; X₅₉ is E, D, Y or A; X₆₀ is S or I; X₆₁ is Q, W, H or A; X₆₂ is S,G, H, Y or N; X₆₃ is N, T, F, H or L; X₆₄ is E, H, T, R or S; X₆₅ is D,F, T, S, L or I; and X₆₆ is Y or L.

The antibody may be an IgG, IgE, IgM, IgD, IgA, and IgY molecule class(for example, IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2) or subclass.

(b) Nucleotide Sequence

Provided herein is an isolated nucleic acid encoding an antibody thatimmunospecifically binds to GP73, a fragment thereof, or a variantthereof. The isolated nucleic acid may comprise a nucleotide sequencethat hybridizes, under stringent conditions, to the nucleic acidmolecule that encodes an antibody comprising the heavy chain or lightchain CDR sequences shown in Table 1. The isolated nucleic acid maycomprise a nucleotide sequence shown in Table 2.

TABLE 2 SEQ ID NUCLEOTIDE NO. SEQUENCE 1B-3440 (VH) 49gaggtgcagcttgttgagactggtggaggattggtgcagcctaaagggtcattgaaactctcatgtgcagcctctggattcaccttcaataccaatgccatgaactgggtccgccaggctccaggaaagggtttggaatgggttgctcgcataaggactaaacgttataattatacaacattttatgccgattcagtgaaagacaggttcaccatctccagagatgattctcaaagcatgctctttctgcaaatgaacaacttgaaaactgaggacacagccatgtattactgtgtgacaggggggactgggacgtttgactactggggccaaggcaccactctcacagtctcctca 1B-3440 (VH) 50 accaatgccatgaac CDR-H1 1B-3440(VH) 51 cgcataaggactaaacgttataattatacaacattttatgccgattcagtgaaagac CDR-H21B-3440 (VH) 52 ggggggactgggacgtttgactac CDR-H3 1B-3440 (VL) 53gaaattgtactcacccagtctccaaccaccatgcctgcatctcccggggagaaggtcactttcacctgcagtgccagctcaggtataagttccaattacttgcattggtatcagctgaagccaggattctcccctaaactatgatttataggacatccaatctggcttctggagtcccagctcgcttcagtggcggtgggtctgggacctatactctctcacaattggcaccatggaggctgaagatgttgccacttactattgccagcagggttttagtataccgctcacgttcggtgctgggaccaagctggagctgaaacgg 1B-3440 (VL) 54agtgccagctcaggtataagttccaattacttgcat CDR-L1 1B-3440 (VL) 55aggacatccaatctggcttct CDR-L2 1B-3440 (VL) 56 cagcagggttttagtataccgctcacgCDR-L3 1B-4971 (VH) 57caggtccagctgcagcagtctggacctgcgctggtaaagcctggggcttcagtgaagatgtcctgcaaggatctggatacacattcactaactatgttatacactgggtgaaacagaagcctgggcagggccttgagcggattggatatatttggccttacaatgatggtactaagttcaatgagaaattcaaaggcaaggccacactgacttcagacaaatcctccagcacagcctacatggagctcagcagcctgacctctgaggactctgcagtctattactgttcaagtcaacagctcgcctactggggccaaggcaccactctcacagtctcctca 1B-4971 (VH) 58 aactatgttatacac CDR-H1 1B-4971 (VH) 59tatatttggccttacaatgatggtactaagttcaatgagaaattcaaaggc CDR-H2 1B-4971 (VH)60 caacagctcgcctac CDR-H3 1B-4971 (VL) 61gatgttgtgatgacccagactccactcactttgtcggttaccattggacaaccagcctccatctcttgcaagtcaagtcagagcctcttatatagtgatggaaagacatatttgatttggttgttacagaggccaggccagtctccaaagcgcctaatctatctggtgtctaaactggactctggagtccctgacaggttcactggcagtggatcagggacagatttcacactgagaatcagcagagtggaggctgaggatttgggagtttattattgttggcaaggtacacattttccgtacacgttcggaggggggaccaagctggaaataaaac gg1B-4971 (VL) 62 aagtcaagtcagagcctcttatatagtgatggaaagacatatttgatt CDR-L11B-4971 (VL) 63 ctggtgtctaaactggactct CDR-L2 1B-4971 (VL) 64tggcaaggtacacattttccgtacacg CDR-L3 1B-3246 (VH) 65gacgtgaagctggtggagtctgggggaggcttagtgaagcctggagggtccctgaaactctcctgtgcagcctctggattcactttcagtagctataccatgtatgggttcgccagactccggagaagagactggagtgggtcgcaaccattagtcgtggtggtacttacatctactatccagacagtgtgaagggccgattcaccatctccagagacaatgccaagaacaccctgtacctgcaaatgagcagtctgaagtctgaggacacagccatatattactgtacaagagaatatttctccggtgatacctacgactactttgactattggggccaaggcaccactctcacagtctcctca 1B-3246 (VH) 66 agctataccatgtct CDR-H11B-3246 (VH) 67 accattagtcgtggtggtacttacatctactatccagacagtgtgaagggcCDR-H2 1B-3246 (VH) 68 gaatatttctccggtgatacctacgactactttgactat CDR-H31B-3246 (VL) 69gagattgtgatgacgcaggctgcattcgccaatccagtcactcttggaacatcagtttccatctcctgcaggtctagtaagagtctcctacatagtaatggcatcacttatttgtactggtatctgcagaagccaggccagtctcctcagctcctgatttatcagatgtccaaccttgcctcaggagtcccagacaggttcagtagcagtgggtcaggaactgatttcacactgagaatcagcagagtggaggctgaggatgtgggtatttactactgtgctcaaaatctagaactttacacgttcggaggggggaccaagctggaaataaaacgg1B-3246 (VL) 70 aggtctagtaagagtctcctacatagtaatggcatcacttatttgtac CDR-L11B-3246 (VL) 71 cagatgtccaaccttgcctca CDR-L2 1B-3246 (VL) 72gctcaaaatctagaactttacacg CDR-L3 1B-4863 (VH) 73caggtgcagctgaaggagtcaggacctggcctggtggcgccctcacagagcctgtccatcacttgcactgtctctgggttttcattaaccagctatggtgtacactgggttcgccagtctccaggaaagggtctggagtggctgggagtaatatggactggtggaagcacaaattataattcggctctcatgtccagactgagcattagtaaagacaactccgagagccaagttttcttaaaagtgaatagtctgcaaactgatgacacaggcatgtactactgtgccagagatcctgggacggactactttgactactggggccaaggcaccactctcacagtctcctca 1B-4863 (VH) 74 agctatggtgtacac CDR-H1 1B-4863 (VH)75 gtaatatggactggtggaagcacaaattataattcggctctcatgtcc CDR-H2 1B-4863 (VH)76 gatcctgggacggactactttgactac CDR-H3 1B-4863 (VL) 77gacattgtgatgacccagtctcacaaattcatgtccacatcaataggagacagggtcagcatctcctgcaaggccagtcaggatgtgagtattgatgtgtcctggtatcaacagaaaccaggacagtctcctacacttctgatttactcggcatcctaccggtacattggagtccctgatcgcttcactggcagtggatctgggacggattcactttcaccatcagcagtgtccaggctgaagacctggcaatttattactgtcagcaacattttactactcctctcacgttcggtgctgggaccaagctggagctgaaacgg 1B-4863 (VL) 78aaggccagtcaggatgtgagtattgatgtgtcc CDR-L1 1B-4863 (VL) 79tcggcatcctaccggtacatt CDR-L2 1B-4863 (VL) 80 cagcaacattttactactcctctcacgCDR-L3 1A-3187 (VH) 81cagggtcagctgcagcagtctggagctgaactgatgaagcctggggcctcagtgaagatatcctgcaaggctactggctacacaatcaggagctactggatagagtgggtaaagcagaggcctggacatggccttgagtggattggagagattttacctggaagtggtaatactaattataatgagaagttcaaggggacggccacattcactgcagatacatcctccaacacagtctatttgcacctcagcagcctgacatctgaggactctgccgtctattactgtgcaaacgggaggggctcctataggtaccactggtttgcttactggggccaagggactctggtcactgtctctcca 1A-3187 (VH) 82 agctactggatagag CDR-H11A-3187 (VH) 83 gagattttacctggaagtggtaatactaattataatgagaagttcaaggggCDR-H2 1A-3187 (VH) 84 gggaggggctcctataggtaccactggtttgcttac CDR-H31A-3187 (VL) 85gacattgtgctgacccaatctccagcttctttggctgtgtctctagggcagagggccaccatctcctgcaaggccagccaaagtgttgattatgatggtgatagttatatgaactggtaccaacagaaaccaggacagccacccaaagtcctcatctatgctgcatccaatctagaatctgggatcccagccaggtttagtggcagtgggtctgggacagacttcaccctcaacatccatcctgtggaggaggaggatgctgcaacctattactgtcagcaaagtaatgaggatccgtacacgttcggaggggggaccaagctggaaatga aacgg1A-3187 (VL) 86 aaggccagccaaagtgttgattatgatggtgatagttatatgaac CDR-L11A-3187 (VL) 87 gctgcatccaatctagaatct CDR-L2 1A-3187 (VL) 88cagcaaagtaatgaggatccgtacacg CDR-L3 1A-4246 (VH) 89gaagtgatgctggtggagtctgggggaggcttagtgaagcctggagggtccctgaaactctcctgtgcagcctctggattcactttcagtagctatgccatgtcttgggttcgcctgactccggagaagaggctggagtgggtcgcaaccattagtagtggtagcagttacacctactatccagacagtgtgaaggggcgattcaccatctccagagacaatgtcaagagcaccctgtacctgcaaatgagcagtctgaggtctgaggacacggccatgtattactgtgcaaggaactgggacggggaactccattactatgctatggactactggggtcaaggaacctcagtcaccgtctcctca 1A-4246 (VH) 90 agctatgccatgtctCDR-H1 1A-4246 (VH) 91accattagtagtggtagcagttacacctactatccagacagtgtgaagggg CDR-H2 1A-4246 (VH)92 aactgggacggggaactccattactatgctatggactac CDR-H3 1A-4246 (VL) 93caaattgttctcacccagtctccagcaatcatgtctgcatctctaggggaacgggtcaccatgacctgcactgccagctcaagtgtaagttccagttacttgcactggtaccagcagaagccaggatcctcccccaaactctgggtttatagcacatccagcctggcttctggagtcccagctcgcttcagtggcagtgggtctgggacctcttactctctcacaatcaacaacatggaggctgaagatgctgccacttatttctgccaccagtatcatcgttccccgtacacgttcggaggggggaccaagctggaaataaaacgg 1A-4246 (VL)94 actgccagctcaagtgtaagttccagttacttgcac CDR-L1 1A-4246 (VL) 95agcacatccagcctggcttct CDR-L2 1A-4246 (VL) 96 caccagtatcatcgttccccgtacacgCDR-L3

c. Antibody Preparation/Production

Antibodies may be prepared by any of a variety of techniques. Ingeneral, antibodies can be produced by cell culture techniques,including the generation of monoclonal antibodies via conventionaltechniques, or via transfection of antibody genes, heavy chains, and/orlight chains into suitable bacterial or mammalian cell hosts, in orderto allow for the production of antibodies, wherein the antibodies may berecombinant. The various forms of the term “transfection” are intendedto encompass a wide variety of techniques commonly used for theintroduction of exogenous DNA into a prokaryotic or eukaryotic hostcell, e.g., electroporation, calcium-phosphate precipitation,DEAE-dextran transfection and the like. Although it is possible toexpress the antibodies of the invention in either prokaryotic oreukaryotic host cells, expression of antibodies in eukaryotic cells ispreferable, and most preferable in mammalian host cells, because sucheukaryotic cells (and in particular mammalian cells) are more likelythan prokaryotic cells to assemble and secrete a properly folded andimmunologically active antibody.

Exemplary mammalian host cells for expressing the recombinant antibodiesof the invention include Chinese Hamster Ovary (CHO cells) (includingdhfr-CHO cells, described in Urlaub and Chasin, Proc. Natl. Acad. Sci.USA, 77: 4216-4220 (1980)), used with a DHFR selectable marker, e.g., asdescribed in Kaufman and Sharp, J. Mol. Biol., 159: 601-621 (1982), NS0myeloma cells, COS cells, and SP2 cells. When recombinant expressionvectors encoding antibody genes are introduced into mammalian hostcells, the antibodies are produced by culturing the host cells for aperiod of time sufficient to allow for expression of the antibody in thehost cells or, more preferably, secretion of the antibody into theculture medium in which the host cells are grown. Antibodies can berecovered from the culture medium using standard protein purificationmethods.

Host cells can also be used to produce functional antibody fragments,such as Fab fragments or scFv molecules. It will be understood thatvariations on the above procedure are within the scope of the presentinvention. For example, it may be desirable to transfect a host cellwith DNA encoding functional fragments of either the light chain and/orthe heavy chain of an antibody of this invention. Recombinant DNAtechnology may also be used to remove some, or all, of the DNA encodingeither or both of the light and heavy chains that is not necessary forbinding to the antigens of interest. The molecules expressed from suchtruncated DNA molecules are also encompassed by the antibodies of theinvention. In addition, bifunctional antibodies may be produced in whichone heavy and one light chain are an antibody of the invention (i.e.,binds human GP73) and the other heavy and light chain are specific foran antigen other than human GP73 by crosslinking an antibody of theinvention to a second antibody by standard chemical crosslinkingmethods.

In a preferred system for recombinant expression of an antibody, orantigen-binding portion thereof, of the invention, a recombinantexpression vector encoding both the antibody heavy chain and theantibody light chain is introduced into dhfr-CHO cells by calciumphosphate-mediated transfection. Within the recombinant expressionvector, the antibody heavy and light chain genes are each operativelylinked to CMV enhancer/AdMLP promoter regulatory elements to drive highlevels of transcription of the genes. The recombinant expression vectoralso carries a DHFR gene, which allows for selection of CHO cells thathave been transfected with the vector using methotrexateselection/amplification. The selected transformant host cells arecultured to allow for expression of the antibody heavy and light chainsand intact antibody is recovered from the culture medium. Standardmolecular biology techniques are used to prepare the recombinantexpression vector, transfect the host cells, select for transformants,culture the host cells, and recover the antibody from the culturemedium. Still further, the invention provides a method of synthesizing arecombinant antibody of the invention by culturing a host cell of theinvention in a suitable culture medium until a recombinant antibody ofthe invention is synthesized. The method can further comprise isolatingthe recombinant antibody from the culture medium.

Methods of preparing monoclonal antibodies involve the preparation ofimmortal cell lines capable of producing antibodies having the desiredspecificity. Such cell lines may be produced from spleen cells obtainedfrom an immunized animal. The animal may be immunized with GP73 or afragment and/or variant thereof. For example, any of SEQ ID NO:97, SEQID NO:98, SEQ ID NO:99, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQID NO:103, SEQ ID NO:104, a fragment of SEQ ID NO:97, SEQ ID NO:98, SEQID NO:99, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, orSEQ ID NO:104, or a variant of SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99,SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, or SEQ IDNO:104 may be used to immunize the animal. The peptide used to immunizethe animal may comprise amino acids encoding human Fc, for example thefragment crystallizable region or tail region of human antibody. Thespleen cells may then be immortalized by, for example, fusion with amyeloma cell fusion partner. A variety of fusion techniques may beemployed. For example, the spleen cells and myeloma cells may becombined with a nonionic detergent for a few minutes and then plated atlow density on a selective medium that supports that growth of hybridcells, but not myeloma cells. One such technique uses hypoxanthine,aminopterin, thymidine (HAT) selection. Another technique includeselectrofusion. After a sufficient time, usually about 1 to 2 weeks,colonies of hybrids are observed. Single colonies are selected and theirculture supernatants tested for binding activity against thepolypeptide. Hybridomas having high reactivity and specificity may beused.

Monoclonal antibodies may be isolated from the supernatants of growinghybridoma colonies. In addition, various techniques may be employed toenhance the yield, such as injection of the hybridoma cell line into theperitoneal cavity of a suitable vertebrate host, such as a mouse.Monoclonal antibodies may then be harvested from the ascites fluid orthe blood. Contaminants may be removed from the antibodies byconventional techniques, such as chromatography, gel filtration,precipitation, and extraction. Affinity chromatography is an example ofa method that can be used in a process to purify the antibodies.

The proteolytic enzyme papain preferentially cleaves IgG molecules toyield several fragments, two of which (the F(ab) fragments) eachcomprise a covalent heterodimer that includes an intact antigen-bindingsite. The enzyme pepsin is able to cleave IgG molecules to provideseveral fragments, including the F(ab′)2 fragment, which comprises bothantigen-binding sites.

The Fv fragment can be produced by preferential proteolytic cleavage ofan IgM, and on rare occasions IgG or IgA immunoglobulin molecules. TheFv fragment may be derived using recombinant techniques. The Fv fragmentincludes a non-covalent VH::VL heterodimer including an antigen-bindingsite that retains much of the antigen recognition and bindingcapabilities of the native antibody molecule.

The antibody, antibody fragment, or derivative may comprise a heavychain and a light chain complementarity determining region (“CDR”) set,respectively interposed between a heavy chain and a light chainframework (“FR”) set which provide support to the CDRs and define thespatial relationship of the CDRs relative to each other. The DR set maycontain three hypervariable regions of a heavy or light chain V region.Proceeding from the N-terminus of a heavy or light chain, these regionsare denoted as “CDR1,” “CDR2,” and “CDR3,” respectively. Anantigen-binding site, therefore, may include six CDRs, comprising theCDR set from each of a heavy and a light chain V region. A polypeptidecomprising a single CDR, (e.g., a CDR1, CDR2, or CDR3) may be referredto as a “molecular recognition unit.” Crystallographic analyses ofantigen-antibody complexes have demonstrated that the amino acidresidues of CDRs form extensive contact with bound antigen, wherein themost extensive antigen contact is with the heavy chain CDR3. Thus, themolecular recognition units may be primarily responsible for thespecificity of an antigen-binding site. In general, the CDR residues aredirectly and most substantially involved in influencing antigen binding.

Other suitable methods of producing or isolating antibodies of therequisite specificity can be used, including, but not limited to,methods that select recombinant antibody from a peptide or proteinlibrary (e.g., but not limited to, a bacteriophage, ribosome,oligonucleotide, RNA, cDNA, yeast or the like, display library); e.g.,as available from various commercial vendors such as Cambridge AntibodyTechnologies (Cambridgeshire, UK), MorphoSys (Martinsreid/Planegg,Del.), Biovation (Aberdeen, Scotland, UK) Bioinvent (Lund, Sweden),using methods known in the art. See U.S. Pat. Nos. 4,704,692; 5,723,323;5,763,192; 5,814,476; 5,817,483; 5,824,514; 5,976,862. Alternativemethods rely upon immunization of transgenic animals (e.g., SCID mice,Nguyen et al. (1997) Microbiol. Immunol. 41:901-907; Sandhu et al.(1996) Crit. Rev. Biotechnol. 16:95-118; Eren et al. (1998) Immunol.93:154-161) that are capable of producing a repertoire of humanantibodies, as known in the art and/or as described herein. Suchtechniques, include, but are not limited to, ribosome display (Hanes etal. (1997) Proc. Natl. Acad. Sci. USA, 94:4937-4942; Hanes et al. (1998)Proc. Natl. Acad. Sci. USA, 95:14130-14135); single cell antibodyproducing technologies (e.g., selected lymphocyte antibody method(“SLAM”) (U.S. Pat. No. 5,627,052, Wen et al. (1987) J. Immunol.17:887-892; Babcook et al. (1996) Proc. Natl. Acad. Sci. USA93:7843-7848); gel microdroplet and flow cytometry (Powell et al. (1990)Biotechnol. 8:333-337; One Cell Systems, (Cambridge, Mass.); Gray et al.(1995) J. 1 mm. Meth. 182:155-163; Kenny et al. (1995) Bio/Technol.13:787-790); B-cell selection (Steenbakkers et al. (1994) Molec. Biol.Reports 19:125-134 (1994)).

An affinity matured antibody may be produced by any one of a number ofprocedures that are known in the art. For example, see Marks et al.,BioTechnology, 10: 779-783 (1992) describes affinity maturation by VHand VL domain shuffling. Random mutagenesis of CDR and/or frameworkresidues is described by Barbas et al., Proc. Nat. Acad. Sci. USA, 91:3809-3813 (1994); Schier et al., Gene, 169: 147-155 (1995); Yelton etal., J. Immunol., 155: 1994-2004 (1995); Jackson et al., J. Immunol.,154(7): 3310-3319 (1995); Hawkins et al, J. Mol. Biol., 226: 889-896(1992). Selective mutation at selective mutagenesis positions and atcontact or hypermutation positions with an activity enhancing amino acidresidue is described in U.S. Pat. No. 6,914,128 B1.

Antibody variants of the present invention can also be prepared usingdelivering a polynucleotide encoding an antibody of this invention to asuitable host such as to provide transgenic animals or mammals, such asgoats, cows, horses, sheep, and the like, that produce such antibodiesin their milk. These methods are known in the art and are described forexample in U.S. Pat. Nos. 5,827,690; 5,849,992; 4,873,316; 5,849,992;5,994,616; 5,565,362; and 5,304,489.

Antibody variants also can be prepared by delivering a polynucleotide ofthis invention to provide transgenic plants and cultured plant cells(e.g., but not limited to tobacco, maize, and duckweed) that producesuch antibodies, specified portions or variants in the plant parts or incells cultured therefrom. For example, Cramer et al. (1999) Curr. Top.Microbol. Immunol. 240:95-118 and references cited therein, describe theproduction of transgenic tobacco leaves expressing large amounts ofrecombinant proteins, e.g., using an inducible promoter. Transgenicmaize have been used to express mammalian proteins at commercialproduction levels, with biological activities equivalent to thoseproduced in other recombinant systems or purified from natural sources.See, e.g., Hood et al., Adv. Exp. Med. Biol. (1999) 464:127-147 andreferences cited therein. Antibody variants have also been produced inlarge amounts from transgenic plant seeds including antibody fragments,such as single chain antibodies (scFv's), including tobacco seeds andpotato tubers. See, e.g., Conrad et al. (1998) Plant Mol. Biol.38:101-109 and reference cited therein. Thus, antibodies of the presentinvention can also be produced using transgenic plants, according toknown methods.

Antibody derivatives can be produced, for example, by adding exogenoussequences to modify immunogenicity or reduce, enhance or modify binding,affinity, on-rate, off-rate, avidity, specificity, half-life, or anyother suitable characteristic. Generally, part or all of the non-humanor human CDR sequences are maintained while the non-human sequences ofthe variable and constant regions are replaced with human or other aminoacids.

Small antibody fragments may be diabodies having two antigen-bindingsites, wherein fragments comprise a heavy chain variable domain (VH)connected to a light chain variable domain (VL) in the same polypeptidechain (VH VL). See for example, EP 404,097; WO 93/11161; and Hollingeret al., (1993) Proc. Natl. Acad. Sci. USA 90:6444-6448. By using alinker that is too short to allow pairing between the two domains on thesame chain, the domains are forced to pair with the complementarydomains of another chain and create two antigen-binding sites. See also,U.S. Pat. No. 6,632,926 to Chen et al. which is hereby incorporated byreference in its entirety and discloses antibody variants that have oneor more amino acids inserted into a hypervariable region of the parentantibody and a binding affinity for a target antigen which is at leastabout two fold stronger than the binding affinity of the parent antibodyfor the antigen.

The antibody may be a linear antibody. The procedure for making a linearantibody is known in the art and described in Zapata et al. (1995)Protein Eng. 8(10):1057-1062. Briefly, these antibodies comprise a pairof tandem Fd segments (VH-CH1-VH-CH1) which form a pair of antigenbinding regions. Linear antibodies can be bispecific or monospecific.

The antibodies may be recovered and purified from recombinant cellcultures by known methods including, but not limited to, protein Apurification, ammonium sulfate or ethanol precipitation, acidextraction, anion or cation exchange chromatography, phosphocellulosechromatography, hydrophobic interaction chromatography, affinitychromatography, hydroxylapatite chromatography and lectinchromatography. High performance liquid chromatography (“HPLC”) can alsobe used for purification.

It may be useful to detectably or therapeutically label the antibody.Methods for conjugating antibodies to these agents are known in the art.For the purpose of illustration only, antibodies can be labeled with adetectable moiety such as a radioactive atom, a chromophore, afluorophore, or the like. Such labeled antibodies can be used fordiagnostic techniques, either in vivo, or in an isolated test sample.Antibodies can also be conjugated, for example, to a pharmaceuticalagent, such as chemotherapeutic drug or a toxin. They can be linked to acytokine, to a ligand, to another antibody. Suitable agents for couplingto antibodies to achieve an anti-tumor effect include cytokines, such asinterleukin 2 (IL-2) and Tumor Necrosis Factor (TNF); photosensitizers,for use in photodynamic therapy, including aluminum (III) phthalocyaninetetrasulfonate, hematoporphyrin, and phthalocyanine; radionuclides, suchas iodine-131 (131I), yttrium-90 (90Y), bismuth-212 (212Bi), bismuth-213(213Bi), technetium-99m (99mTc), rhenium-186 (186Re), and rhenium-188(188Re); antibiotics, such as doxorubicin, adriamycin, daunorubicin,methotrexate, daunomycin, neocarzinostatin, and carboplatin; bacterial,plant, and other toxins, such as diphtheria toxin, pseudomonas exotoxinA, staphylococcal enterotoxin A, abrin-A toxin, ricin A (deglycosylatedricin A and native ricin A), TGF-alpha toxin, cytotoxin from chinesecobra (naja naja atra), and gelonin (a plant toxin); ribosomeinactivating proteins from plants, bacteria and fungi, such asrestrictocin (a ribosome inactivating protein produced by Aspergillusrestrictus), saporin (a ribosome inactivating protein from Saponariaofficinalis), and RNase; tyrosine kinase inhibitors; ly207702 (adifluorinated purine nucleoside); liposomes containing anti cysticagents (e.g., antisense oligonucleotides, plasmids which encode fortoxins, methotrexate, etc.); and other antibodies or antibody fragments,such as F(ab).

The antibodies can be sequenced and replicated by recombinant orsynthetic means. They also can be further sequenced down to the linearsequence of nucleotides that encode them. Accordingly, this inventionprovides these polynucleotides, alone or in combination with a carrier,vector or host cell as described above, that encode a sequence of anantibody of this invention.

Antibody production via the use of hybridoma technology, the selectedlymphocyte antibody method (SLAM), transgenic animals, and recombinantantibody libraries is described in more detail below.

(1) Anti-GP73 Monoclonal Antibodies Using Hybridoma Technology

Monoclonal antibodies can be prepared using a wide variety of techniquesknown in the art including the use of hybridoma, recombinant, and phagedisplay technologies, or a combination thereof. For example, monoclonalantibodies can be produced using hybridoma techniques including thoseknown in the art and taught, for example, in Harlow et al., Antibodies:A Laboratory Manual, second edition, (Cold Spring Harbor LaboratoryPress, Cold Spring Harbor, 1988); Hammerling, et al., In MonoclonalAntibodies and T-Cell Hybridomas, (Elsevier, N.Y., 1981). It is alsonoted that the term “monoclonal antibody” as used herein is not limitedto antibodies produced through hybridoma technology. The term“monoclonal antibody” refers to an antibody that is derived from asingle clone, including any eukaryotic, prokaryotic, or phage clone, andnot the method by which it is produced.

In an embodiment, the present invention provides methods of generatingmonoclonal antibodies as well as antibodies produced by the method. Themethod may comprise culturing a hybridoma cell secreting an antibody ofthe invention wherein, preferably, the hybridoma is generated by fusingsplenocytes isolated from an animal, e.g., a rat or a mouse, immunizedwith GP73 with myeloma cells and then screening the hybridomas resultingfrom the fusion for hybridoma clones that secrete an antibody able tobind a polypeptide of the invention. Briefly, rats can be immunized witha GP73 antigen. In a preferred embodiment, the GP73 antigen isadministered with an adjuvant to stimulate the immune response. Suchadjuvants include complete or incomplete Freund's adjuvant, RIBI(muramyl dipeptides) or ISCOM (immunostimulating complexes). Suchadjuvants may protect the polypeptide from rapid dispersal bysequestering it in a local deposit, or they may contain substances thatstimulate the host to secrete factors that are chemotactic formacrophages and other components of the immune system. Preferably, if apolypeptide is being administered, the immunization schedule willinvolve two or more administrations of the polypeptide, spread out overseveral weeks; however, a single administration of the polypeptide mayalso be used.

After immunization of an animal with a GP73 antigen, antibodies and/orantibody-producing cells may be obtained from the animal. An anti-GP73antibody-containing serum is obtained from the animal by bleeding orsacrificing the animal. The serum may be used as it is obtained from theanimal, an immunoglobulin fraction may be obtained from the serum, orthe anti-GP73 antibodies may be purified from the serum. Serum orimmunoglobulins obtained in this manner are polyclonal, thus having aheterogeneous array of properties.

Once an immune response is detected, e.g., antibodies specific for theantigen GP73 are detected in the rat serum, the rat spleen is harvestedand splenocytes isolated. The splenocytes are then fused by well-knowntechniques to any suitable myeloma cells, for example, cells from cellline SP20 available from the American Type Culture Collection (ATCC,Manassas, Va., US). Hybridomas are selected and cloned by limiteddilution. The hybridoma clones are then assayed by methods known in theart for cells that secrete antibodies capable of binding GP73. Ascitesfluid, which generally contains high levels of antibodies, can begenerated by immunizing rats with positive hybridoma clones.

In another embodiment, antibody-producing immortalized hybridomas may beprepared from the immunized animal. After immunization, the animal issacrificed and the splenic B cells are fused to immortalized myelomacells as is well known in the art. See, e.g., Harlow and Lane, supra. Ina preferred embodiment, the myeloma cells do not secrete immunoglobulinpolypeptides (a non-secretory cell line). After fusion and antibioticselection, the hybridomas are screened using GP73, or a portion thereof,or a cell expressing GP73. In a preferred embodiment, the initialscreening is performed using an enzyme-linked immunosorbent assay(ELISA) or a radioimmunoassay (RIA), preferably an ELISA. An example ofELISA screening is provided in PCT Publication No. WO 00/37504.

Anti-GP73 antibody-producing hybridomas are selected, cloned, andfurther screened for desirable characteristics, including robusthybridoma growth, high antibody production, and desirable antibodycharacteristics. Hybridomas may be cultured and expanded in vivo insyngeneic animals, in animals that lack an immune system, e.g., nudemice, or in cell culture in vitro. Methods of selecting, cloning andexpanding hybridomas are well known to those of ordinary skill in theart.

In a preferred embodiment, hybridomas are rat hybridomas. In anotherembodiment, hybridomas are produced in a non-human, non-rat species suchas mice, sheep, pigs, goats, cattle, or horses. In yet another preferredembodiment, the hybridomas are human hybridomas, in which a humannon-secretory myeloma is fused with a human cell expressing an anti-GP73antibody.

Antibody fragments that recognize specific epitopes may be generated byknown techniques. For example, Fab and F(ab′)2 fragments of theinvention may be produced by proteolytic cleavage of immunoglobulinmolecules, using enzymes such as papain (to produce two identical Fabfragments) or pepsin (to produce an F(ab′)₂ fragment). A F(ab′)2fragment of an IgG molecule retains the two antigen-binding sites of thelarger (“parent”) IgG molecule, including both light chains (containingthe variable light chain and constant light chain regions), the CH1domains of the heavy chains, and a disulfide-forming hinge region of theparent IgG molecule. Accordingly, an F(ab′)2 fragment is still capableof crosslinking antigen molecules like the parent IgG molecule.

(2) Anti-GP73 Monoclonal Antibodies Using SLAM

In another aspect of the invention, recombinant antibodies are generatedfrom single, isolated lymphocytes using a procedure referred to in theart as the selected lymphocyte antibody method (SLAM), as described inU.S. Pat. No. 5,627,052; PCT Publication No. WO 92/02551; and Babcook etal., Proc. Natl. Acad. Sci. USA, 93: 7843-7848 (1996). In this method,single cells secreting antibodies of interest, e.g., lymphocytes derivedfrom any one of the immunized animals are screened using anantigen-specific hemolytic plaque assay, wherein the antigen GP73, asubunit of GP73, or a fragment thereof, is coupled to sheep red bloodcells using a linker, such as biotin, and used to identify single cellsthat secrete antibodies with specificity for GP73. Followingidentification of antibody-secreting cells of interest, heavy- andlight-chain variable region cDNAs are rescued from the cells by reversetranscriptase-PCR (RT-PCR) and these variable regions can then beexpressed, in the context of appropriate immunoglobulin constant regions(e.g., human constant regions), in mammalian host cells, such as COS orCHO cells. The host cells transfected with the amplified immunoglobulinsequences, derived from in vivo selected lymphocytes, can then undergofurther analysis and selection in vitro, for example, by panning thetransfected cells to isolate cells expressing antibodies to GP73. Theamplified immunoglobulin sequences further can be manipulated in vitro,such as by in vitro affinity maturation method. See, for example, PCTPublication No. WO 97/29131 and PCT Publication No. WO 00/56772.

(3) Anti-GP73 Monoclonal Antibodies Using Transgenic Animals

In another embodiment of the invention, antibodies are produced byimmunizing a non-human animal comprising some, or all, of the humanimmunoglobulin locus with a GP73 antigen. In an embodiment, thenon-human animal is a XENOMOUSE® transgenic mouse, an engineered mousestrain that comprises large fragments of the human immunoglobulin lociand is deficient in mouse antibody production. See, e.g., Green et al.,Nature Genetics, 7: 13-21 (1994) and U.S. Pat. Nos. 5,916,771;5,939,598; 5,985,615; 5,998,209; 6,075,181; 6,091,001; 6,114,598; and6,130,364. See also PCT Publication Nos. WO 91/10741; WO 94/02602; WO96/34096; WO 96/33735; WO 98/16654; WO 98/24893; WO 98/50433; WO99/45031; WO 99/53049; WO 00/09560; and WO 00/37504. The XENOMOUSE®transgenic mouse produces an adult-like human repertoire of fully humanantibodies, and generates antigen-specific human monoclonal antibodies.The XENOMOUSE® transgenic mouse contains approximately 80% of the humanantibody repertoire through introduction of megabase sized, germlineconfiguration YAC fragments of the human heavy chain loci and x lightchain loci. See Mendez et al., Nature Genetics, 15: 146-156 (1997),Green and Jakobovits, J. Exp. Med., 188: 483-495 (1998), the disclosuresof which are hereby incorporated by reference.

(4) Anti-GP73 Monoclonal Antibodies Using Recombinant Antibody Libraries

In vitro methods also can be used to make the antibodies of theinvention, wherein an antibody library is screened to identify anantibody having the desired GP73-binding specificity. Methods for suchscreening of recombinant antibody libraries are well known in the artand include methods described in, for example, U.S. Pat. No. 5,223,409(Ladner et al.); PCT Publication No. WO 92/18619 (Kang et al.); PCTPublication No. WO 91/17271 (Dower et al.); PCT Publication No. WO92/20791 (Winter et al.); PCT Publication No. WO 92/15679 (Markland etal.); PCT Publication No. WO 93/01288 (Breitling et al.); PCTPublication No. WO 92/01047 (McCafferty et al.); PCT Publication No. WO92/09690 (Garrard et al.); Fuchs et al., Bio/Technology, 9: 1369-1372(1991); Hay et al., Hum. Antibod. Hybridomas, 3: 81-85 (1992); Huse etal., Science, 246: 1275-1281 (1989); McCafferty et al., Nature, 348:552-554 (1990); Griffiths et al., EMBO J., 12: 725-734 (1993); Hawkinset al., J. Mol. Biol., 226: 889-896 (1992); Clackson et al., Nature,352: 624-628 (1991); Gram et al., Proc. Natl. Acad. Sci. USA, 89:3576-3580 (1992); Garrard et al., Bio/Technology, 9: 1373-1377 (1991);Hoogenboom et al., Nucl. Acids Res., 19: 4133-4137 (1991); Barbas etal., Proc. Natl. Acad. Sci. USA, 88: 7978-7982 (1991); US PatentApplication Publication No. 2003/0186374; and PCT Publication No. WO97/29131, the contents of each of which are incorporated herein byreference.

The recombinant antibody library may be from a subject immunized withGP73, or a portion of GP73. Alternatively, the recombinant antibodylibrary may be from a naive subject, i.e., one who has not beenimmunized with GP73, such as a human antibody library from a humansubject who has not been immunized with human GP73. Antibodies of theinvention are selected by screening the recombinant antibody librarywith the peptide comprising human GP73 to thereby select thoseantibodies that recognize GP73. Methods for conducting such screeningand selection are well known in the art, such as described in thereferences in the preceding paragraph. To select antibodies of theinvention having particular binding affinities for GP73, such as thosethat dissociate from human GP73 with a particular K_(off) rate constant,the art-known method of surface plasmon resonance can be used to selectantibodies having the desired K_(off) rate constant. To selectantibodies of the invention having a particular neutralizing activityfor hGP73, such as those with a particular IC₅₀, standard methods knownin the art for assessing the inhibition of GP73 activity may be used.

In one aspect, the invention pertains to an isolated antibody, or anantigen-binding portion thereof, that binds human GP73. Preferably, theantibody is a neutralizing antibody. In various embodiments, theantibody is a recombinant antibody or a monoclonal antibody.

For example, antibodies of the present invention can also be generatedusing various phage display methods known in the art. In phage displaymethods, functional antibody domains are displayed on the surface ofphage particles which carry the polynucleotide sequences encoding them.Such phage can be utilized to display antigen-binding domains expressedfrom a repertoire or combinatorial antibody library (e.g., human ormurine). Phage expressing an antigen binding domain that binds theantigen of interest can be selected or identified with antigen, e.g.,using labeled antigen or antigen bound or captured to a solid surface orbead. Phage used in these methods are typically filamentous phageincluding fd and M13 binding domains expressed from phage with Fab, Fv,or disulfide stabilized Fv antibody domains recombinantly fused toeither the phage gene III or gene VIII protein. Examples of phagedisplay methods that can be used to make the antibodies of the presentinvention include those disclosed in Brinkmann et al., J. Immunol.Methods, 182: 41-50 (1995); Ames et al., J. Immunol. Methods,184:177-186 (1995); Kettleborough et al., Eur. J. Immunol., 24: 952-958(1994); Persic et al., Gene, 187: 9-18 (1997); Burton et al., Advancesin Immunology, 57: 191-280 (1994); PCT Publication No. WO 92/01047; PCTPublication Nos. WO 90/02809; WO 91/10737; WO 92/01047; WO 92/18619; WO93/11236; WO 95/15982; WO 95/20401; and U.S. Pat. Nos. 5,698,426;5,223,409; 5,403,484; 5,580,717; 5,427,908; 5,750,753; 5,821,047;5,571,698; 5,427,908; 5,516,637; 5,780,225; 5,658,727; 5,733,743; and5,969,108.

As described in the above references, after phage selection, theantibody coding regions from the phage can be isolated and used togenerate whole antibodies including human antibodies or any otherdesired antigen binding fragment, and expressed in any desired host,including mammalian cells, insect cells, plant cells, yeast, andbacteria, e.g., as described in detail below. For example, techniques torecombinantly produce Fab, Fab′, and F(ab′)₂ fragments can also beemployed using methods known in the art such as those disclosed in PCTpublication No. WO 92/22324; Mullinax et al., BioTechniques, 12(6):864-869 (1992); Sawai et al., Am. J. Reprod. Immunol., 34: 26-34 (1995);and Better et al., Science, 240: 1041-1043 (1988). Examples oftechniques which can be used to produce single-chain Fvs and antibodiesinclude those described in U.S. Pat. Nos. 4,946,778 and 5,258,498;Huston et al., Methods in Enzymology, 203: 46-88 (1991); Shu et al.,Proc. Natl. Acad. Sci. USA, 90: 7995-7999 (1993); and Skerra et al.,Science, 240: 1038-1041 (1988).

Alternative to screening of recombinant antibody libraries by phagedisplay, other methodologies known in the art for screening largecombinatorial libraries can be applied to the identification ofantibodies of the invention. One type of alternative expression systemis one in which the recombinant antibody library is expressed asRNA-protein fusions, as described in PCT Publication No. WO 98/31700(Szostak and Roberts), and in Roberts and Szostak, Proc. Natl. Acad.Sci. USA, 94: 12297-12302 (1997). In this system, a covalent fusion iscreated between an mRNA and the peptide or protein that it encodes by invitro translation of synthetic mRNAs that carry puromycin, a peptidylacceptor antibiotic, at their 3′ end. Thus, a specific mRNA can beenriched from a complex mixture of mRNAs (e.g., a combinatorial library)based on the properties of the encoded peptide or protein, e.g.,antibody, or portion thereof, such as binding of the antibody, orportion thereof, to the dual specificity antigen. Nucleic acid sequencesencoding antibodies, or portions thereof, recovered from screening ofsuch libraries can be expressed by recombinant means as described above(e.g., in mammalian host cells) and, moreover, can be subjected tofurther affinity maturation by either additional rounds of screening ofmRNA-peptide fusions in which mutations have been introduced into theoriginally selected sequence(s), or by other methods for affinitymaturation in vitro of recombinant antibodies, as described above. Apreferred example of this methodology, is PROfusion display technology.

In another approach, the antibodies of the present invention can also begenerated using yeast display methods known in the art. In yeast displaymethods, genetic methods are used to tether antibody domains to theyeast cell wall and display them on the surface of yeast. In particular,such yeast can be utilized to display antigen-binding domains expressedfrom a repertoire or combinatorial antibody library (e.g., human ormurine). Examples of yeast display methods that can be used to make theantibodies of the present invention include those disclosed in U.S. Pat.No. 6,699,658 (Wittrup et al.) incorporated herein by reference.

d. Production of Recombinant GP73 Antibodies

Antibodies of the present invention may be produced by any of a numberof techniques known in the art. For example, expression from host cells,wherein expression vector(s) encoding the heavy and light chains is(are) transfected into a host cell by standard techniques. The variousforms of the term “transfection” are intended to encompass a widevariety of techniques commonly used for the introduction of exogenousDNA into a prokaryotic or eukaryotic host cell, e.g., electroporation,calcium-phosphate precipitation, DEAE-dextran transfection, and thelike. Although it is possible to express the antibodies of the inventionin either prokaryotic or eukaryotic host cells, expression of antibodiesin eukaryotic cells is preferable, and most preferable in mammalian hostcells, because such eukaryotic cells (and in particular mammalian cells)are more likely than prokaryotic cells to assemble and secrete aproperly folded and immunologically active antibody.

Exemplary mammalian host cells for expressing the recombinant antibodiesof the invention include Chinese Hamster Ovary (CHO cells) (includingdhfr-CHO cells, described in Urlaub and Chasin, Proc. Natl. Acad. Sci.USA, 77: 4216-4220 (1980), used with a DHFR selectable marker, e.g., asdescribed in Kaufman and Sharp, J. Mol. Biol., 159: 601-621 (1982), NS0myeloma cells, COS cells, and SP2 cells. When recombinant expressionvectors encoding antibody genes are introduced into mammalian hostcells, the antibodies are produced by culturing the host cells for aperiod of time sufficient to allow for expression of the antibody in thehost cells or, more preferably, secretion of the antibody into theculture medium in which the host cells are grown. Antibodies can berecovered from the culture medium using standard protein purificationmethods.

Host cells can also be used to produce functional antibody fragments,such as Fab fragments or scFv molecules. It will be understood thatvariations on the above procedure are within the scope of the presentinvention. For example, it may be desirable to transfect a host cellwith DNA encoding functional fragments of either the light chain and/orthe heavy chain of an antibody of this invention. Recombinant DNAtechnology may also be used to remove some, or all, of the DNA encodingeither or both of the light and heavy chains that is not necessary forbinding to the antigens of interest. The molecules expressed from suchtruncated DNA molecules are also encompassed by the antibodies of theinvention. In addition, bifunctional antibodies may be produced in whichone heavy and one light chain are an antibody of the invention (i.e.,binds human GP73) and the other heavy and light chain are specific foran antigen other than human GP73 by crosslinking an antibody of theinvention to a second antibody by standard chemical crosslinkingmethods.

In a preferred system for recombinant expression of an antibody, orantigen-binding portion thereof, of the invention, a recombinantexpression vector encoding both the antibody heavy chain and theantibody light chain is introduced into dhfr-CHO cells by calciumphosphate-mediated transfection. Within the recombinant expressionvector, the antibody heavy and light chain genes are each operativelylinked to CMV enhancer/AdMLP promoter regulatory elements to drive highlevels of transcription of the genes. The recombinant expression vectoralso carries a DHFR gene, which allows for selection of CHO cells thathave been transfected with the vector using methotrexateselection/amplification. The selected transformant host cells arecultured to allow for expression of the antibody heavy and light chainsand intact antibody is recovered from the culture medium. Standardmolecular biology techniques are used to prepare the recombinantexpression vector, transfect the host cells, select for transformants,culture the host cells, and recover the antibody from the culturemedium. Still further, the invention provides a method of synthesizing arecombinant antibody of the invention by culturing a host cell of theinvention in a suitable culture medium until a recombinant antibody ofthe invention is synthesized. The method can further comprise isolatingthe recombinant antibody from the culture medium.

(1) Humanized Antibody

The humanized antibody may be an antibody or a variant, derivative,analog or portion thereof which immunospecifically binds to an antigenof interest and which comprises a framework (FR) region havingsubstantially the amino acid sequence of a human antibody and acomplementary determining region (CDR) having substantially the aminoacid sequence of a non-human antibody. The humanized antibody may befrom a non-human species antibody that binds the desired antigen havingone or more complementarity determining regions (CDRs) from thenon-human species and framework regions from a human immunoglobulinmolecule.

As used herein, the term “substantially” in the context of a CDR refersto a CDR having an amino acid sequence at least 90%, at least 95%, atleast 98% or at least 99% identical to the amino acid sequence of anon-human antibody CDR. A humanized antibody comprises substantially allof at least one, and typically two, variable domains (Fab, Fab′,F(ab′)2, FabC, Fv) in which all or substantially all of the CDR regionscorrespond to those of a non-human immunoglobulin (i.e., donor antibody)and all or substantially all of the framework regions are those of ahuman immunoglobulin consensus sequence. According to one aspect, ahumanized antibody also comprises at least a portion of animmunoglobulin constant region (Fc), typically that of a humanimmunoglobulin. In some embodiments, a humanized antibody contains boththe light chain as well as at least the variable domain of a heavychain. The antibody also may include the CH1, hinge, CH2, CH3, and CH4regions of the heavy chain. In some embodiments, a humanized antibodyonly contains a humanized light chain. In some embodiments, a humanizedantibody only contains a humanized heavy chain. In specific embodiments,a humanized antibody only contains a humanized variable domain of alight chain and/or of a heavy chain.

The humanized antibody can be selected from any class ofimmunoglobulins, including IgM, IgG, IgD, IgA and IgE, and any isotype,including without limitation IgG 1, IgG2, IgG3, and IgG4. The humanizedantibody may comprise sequences from more than one class or isotype, andparticular constant domains may be selected to optimize desired effectorfunctions using techniques well-known in the art.

The framework and CDR regions of a humanized antibody need notcorrespond precisely to the parental sequences, e.g., the donor antibodyCDR or the consensus framework may be mutagenized by substitution,insertion and/or deletion of at least one amino acid residue so that theCDR or framework residue at that site does not correspond to either thedonor antibody or the consensus framework. In one embodiment, suchmutations, however, will not be extensive. Usually, at least 90%, atleast 95%, at least 98%, or at least 99% of the humanized antibodyresidues will correspond to those of the parental FR and CDR sequences.As used herein, the term “consensus framework” refers to the frameworkregion in the consensus immunoglobulin sequence. As used herein, theterm “consensus immunoglobulin sequence” refers to the sequence formedfrom the most frequently occurring amino acids (or nucleotides) in afamily of related immunoglobulin sequences (See e.g., Winnaker, FromGenes to Clones (Verlagsgesellschaft, Weinheim, Germany 1987)). In afamily of immunoglobulins, each position in the consensus sequence isoccupied by the amino acid occurring most frequently at that position inthe family. If two amino acids occur equally frequently, either can beincluded in the consensus sequence.

The humanized antibody may be designed to minimize unwantedimmunological response toward rodent anti-human antibodies, which limitsthe duration and effectiveness of therapeutic applications of thosemoieties in human recipients. The humanized antibody may have one ormore amino acid residues introduced into it from a source that isnon-human. These non-human residues are often referred to as “import”residues, which are typically taken from a variable domain. Humanizationmay be performed by substituting hypervariable region sequences for thecorresponding sequences of a human antibody. Accordingly, such“humanized” antibodies are chimeric antibodies wherein substantiallyless than an intact human variable domain has been substituted by thecorresponding sequence from a non-human species. For example, see U.S.Pat. No. 4,816,567, the contents of which are herein incorporated byreference. The humanized antibody may be a human antibody in which somehypervariable region residues, and possibly some FR residues aresubstituted by residues from analogous sites in rodent antibodies.Humanization or engineering of antibodies of the present invention canbe performed using any known method, such as but not limited to thosedescribed in U.S. Pat. Nos. 5,723,323; 5,976,862; 5,824,514; 5,817,483;5,814,476; 5,763,192; 5,723,323; 5,766,886; 5,714,352; 6,204,023;6,180,370; 5,693,762; 5,530,101; 5,585,089; 5,225,539; and 4,816,567.

The humanized antibody may retain high affinity for GP73 and otherfavorable biological properties. The humanized antibody may be preparedby a process of analysis of the parental sequences and variousconceptual humanized products using three-dimensional models of theparental and humanized sequences. Three-dimensional immunoglobulinmodels are commonly available. Computer programs are available thatillustrate and display probable three-dimensional conformationalstructures of selected candidate immunoglobulin sequences. Inspection ofthese displays permits analysis of the likely role of the residues inthe functioning of the candidate immunoglobulin sequence, i.e., theanalysis of residues that influence the ability of the candidateimmunoglobulin to bind its antigen. In this way, FR residues can beselected and combined from the recipient and import sequences so thatthe desired antibody characteristics, such as increased affinity forGP73, is achieved. In general, the hypervariable region residues may bedirectly and most substantially involved in influencing antigen binding.

As an alternative to humanization, human antibodies (also referred toherein as “fully human antibodies”) can be generated. For example, it ispossible to isolate human antibodies from libraries via PROfusion and/oryeast related technologies. It is also possible to produce transgenicanimals (e.g. mice that are capable, upon immunization, of producing afull repertoire of human antibodies in the absence of endogenousimmunoglobulin production. For example, the homozygous deletion of theantibody heavy-chain joining region (J_(H)) gene in chimeric andgerm-line mutant mice results in complete inhibition of endogenousantibody production. Transfer of the human germ-line immunoglobulin genearray in such germ-line mutant mice will result in the production ofhuman antibodies upon antigen challenge. The humanized or fully humanantibodies may be prepared according to the methods described in U.S.Pat. Nos. 5,770,429; 5,833,985; 5,837,243; 5,922,845; 6,017,517;6,096,311; 6,111,166; 6,270,765; 6,303,755; 6,365,116; 6,410,690;6,682,928; and 6,984,720, the contents each of which are hereinincorporated by reference.

e. Anti-GP73 Antibodies

Anti-GP73 antibodies may be generated using the techniques describedabove. The anti-GP73 antibody may be a 1B-3440 monoclonal antibody, a1B-4971 monoclonal antibody, a 1B-3246 monoclonal antibody, a 1B-4863monoclonal antibody, a 1A-3187 monoclonal antibody, a 1A-4246 monoclonalantibody, or antibody fragments thereof

(1) 1B-3440

As used herein, “1B-3440” or “mAb 1B-3440” refers to a monoclonalantibody produced by hybridoma cell line that was made using RBF/DnJmice strain that was immunized with recombinant GP73. The spleen cellsof the mice were fused with mouse myeloma cell line NS/0 myeloma cells.

1B-3440 binds to an epitope on GP73 that is different from the epitopesthat 1B-4971, 1B-3246, 1B-4863, 1A-3187, and 1A-4246 bind. 1B-3440recognizes the epitope peptide of SEQ ID NO: 67. 1B-3440 has a bindingaffinity (K_(D)) for GP73 of 9.1×10⁻¹¹ M. 1B-3440 has a heavy chainamino acid sequence of SEQ ID NO: 1, which is encoded by a nucleotidesequence of SEQ ID NO: 49, and a light chain amino acid sequence of SEQID NO: 5, which is encoded by a nucleotide sequence of SEQ ID NO: 53.1B-3440 includes CDR-H1 (SEQ ID NO: 2), CDR-H2 (SEQ ID NO: 3), andCDR-H3 (SEQ ID NO: 4) and CDR-L1 (SEQ ID NO: 6), CDR-L2 (SEQ ID NO: 7),and CDR-L3 (SEQ ID NO: 8), which are encoded by nucleotide sequences ofSEQ ID NOs: 50-52 and 54-56, respectively.

(2) 1B-4971

As used herein, “1B-4971” or “mAb 1B-4971” refers to a monoclonalantibody produced by hybridoma cell line that was made using RBF/DnJmice strain that was immunized with recombinant GP73. The spleen cellsof the mice were fused with mouse myeloma cell line NS/0 myeloma cells.

1B-4971 binds to an epitope on GP73 that is different from the epitopesthat 1B-3440, 1B-3246, 1B-4863, 1A-3187, and 1A-4246 bind. 1B-4971recognizes the epitope peptide of SEQ ID NO: 65. 1B-4971 has a bindingaffinity (K_(D)) for GP73 of 2.0×10⁻¹² M. 1B-4971 has a heavy chainamino acid sequence of SEQ ID NO: 9, which is encoded by a nucleotidesequence of SEQ ID NO: 57, and a light chain amino acid sequence of SEQID NO: 13, which is encoded by a nucleotide sequence of SEQ ID NO: 61.1B-3440 includes CDR-H1 (SEQ ID NO: 10), CDR-H2 (SEQ ID NO: 11), andCDR-H3 (SEQ ID NO: 12) and CDR-L1 (SEQ ID NO: 14), CDR-L2 (SEQ ID NO:15), and CDR-L3 (SEQ ID NO: 16), which are encoded by nucleotidesequences of SEQ ID NOs: 58-60 and 62-64, respectively.

(3) 1B-3246

As used herein, “1B-3246” or “mAb 1B-3246” refers to a monoclonalantibody produced by hybridoma cell line that was made using RBF/DnJmice strain that was immunized with recombinant GP73. The spleen cellsof the mice were fused with mouse myeloma cell line NS/0 myeloma cells.

1B-3246 binds to an epitope on GP73 that is different from the epitopesthat 1B-3440, 1B-4971, 1B-4863, 1A-3187, and 1A-4246 bind. 1B-3246recognizes the epitope peptide of SEQ ID NO: 66. 1B-3246 has a bindingaffinity (K_(D)) for GP73 of 7.9×10⁻¹¹ M. 1B-3246 has a heavy chainamino acid sequence of SEQ ID NO: 17, which is encoded by a nucleotidesequence of SEQ ID NO: 65, and a light chain amino acid sequence of SEQID NO: 21, which is encoded by a nucleotide sequence of SEQ ID NO: 69.1B-3246 includes CDR-H1 (SEQ ID NO: 18), CDR-H2 (SEQ ID NO: 19), andCDR-H3 (SEQ ID NO: 20) and CDR-L1 (SEQ ID NO: 22), CDR-L2 (SEQ ID NO:23), and CDR-L3 (SEQ ID NO: 24), which are encoded by nucleotidesequences of SEQ ID NOs: 66-68 and 70-72, respectively.

(4) 1B-4863

As used herein, “1B-4863” or “mAb 1B-4863” refers to a monoclonalantibody produced by hybridoma cell line that was made using RBF/DnJmice strain that was immunized with recombinant GP73. The spleen cellsof the mice were fused with mouse myeloma cell line NS/0 myeloma cells.

1B-4863 binds to an epitope on GP73 that is different from the epitopesthat 1B-3440, 1B-4971, 1B-3246, 1A-3187, and 1A-4246 bind. 1B-4863 has abinding affinity (K_(D)) for GP73 of 1.2×10⁻¹⁰ M. 1B-4863 has a heavychain amino acid sequence of SEQ ID NO: 25, which is encoded by anucleotide sequence of SEQ ID NO: 73, and a light chain amino acidsequence of SEQ ID NO: 29, which is encoded by a nucleotide sequence ofSEQ ID NO: 77. 1B-4863 includes CDR-H1 (SEQ ID NO: 26), CDR-H2 (SEQ IDNO: 27), and CDR-H3 (SEQ ID NO: 28) and CDR-L1 (SEQ ID NO: 30), CDR-L2(SEQ ID NO: 31), and CDR-L3 (SEQ ID NO: 32), which are encoded bynucleotide sequences of SEQ ID NOs: 74-76 and 78-80, respectively.

(5) 1A-3187

As used herein, “1A-3187” or “mAb 1A-3187” refers to a monoclonalantibody produced by hybridoma cell line that was made using CAF1/J micestrain that was immunized with recombinant GP73. The spleen cells of themice were fused with mouse myeloma cell line NS/0 myeloma cells.

1A-3187 binds to an epitope on GP73 that is different from the epitopesthat 1B-3440, 1B-4971, 1B-3246, 1B-4863, and 1A-4246 bind. 1A-3187recognizes the epitope peptide of SEQ ID NO: 68. 1A-3187 has a bindingaffinity (K_(D)) for GP73 of 2.0×10⁻⁹ M. 1A-3187 has a heavy chain aminoacid sequence of SEQ ID NO: 33, which is encoded by a nucleotidesequence of SEQ ID NO: 81, and a light chain amino acid sequence of SEQID NO: 37, which is encoded by a nucleotide sequence of SEQ ID NO: 85.1A-3187 includes CDR-H1 (SEQ ID NO: 34), CDR-H2 (SEQ ID NO: 35), andCDR-H3 (SEQ ID NO: 36) and CDR-L1 (SEQ ID NO: 38), CDR-L2 (SEQ ID NO:39), and CDR-L3 (SEQ ID NO: 40), which are encoded by nucleotidesequences of SEQ ID NOs: 82-84 and 86-88, respectively.

(6) 1A-4246

As used herein, “1A-4246” or “mAB 1A-4246” refers to a monoclonalantibody produced by hybridoma cell line that was made using CAF1/J micestrain that was immunized with recombinant GP73. The spleen cells of themice were fused with mouse myeloma cell line NS/0 myeloma cells.

1A-4246 binds to an epitope on GP73 that is different from the epitopesthat 1B-3440, 1B-4971, 1B-3246, 1B-4863, and 1A-3187 bind. 1A-4246recognizes the epitope peptide of SEQ ID NO: 67. 1A-4246 has a bindingaffinity (K_(D)) for GP73 of 3.8×10⁻⁹ M. 1A-4246 has a heavy chain aminoacid sequence of SEQ ID NO: 41, which is encoded by a nucleotidesequence of SEQ ID NO: 89, and a light chain amino acid sequence of SEQID NO: 45, which is encoded by a nucleotide sequence of SEQ ID NO: 93.1A-4246 includes CDR-H1 (SEQ ID NO: 42), CDR-H2 (SEQ ID NO: 43), andCDR-H3 (SEQ ID NO: 44) and CDR-L1 (SEQ ID NO: 46), CDR-L2 (SEQ ID NO:47), and CDR-L3 (SEQ ID NO: 48), which are encoded by nucleotidesequences of SEQ ID NOs: 90-92 and 94-96, respectively.

3. PHARMACEUTICAL COMPOSITIONS

The antibody may be a component in a pharmaceutical composition. Thepharmaceutical composition may also contain a pharmaceuticallyacceptable carrier. The pharmaceutical compositions comprisingantibodies of the invention are for use in, but not limited to,diagnosing, detecting, or monitoring a disorder, in preventing,treating, managing, or ameliorating of a disorder or one or moresymptoms thereof, and/or in research. In a specific embodiment, acomposition comprises one or more antibodies of the invention. Inanother embodiment, the pharmaceutical composition comprises one or moreantibodies of the invention and one or more prophylactic or therapeuticagents other than antibodies of the invention for treating a disorder inwhich activity of a targeted GP73 is detrimental. In a furtherembodiment, the prophylactic or therapeutic agents are known to beuseful for, or have been, or are currently being used in the prevention,treatment, management, or amelioration of a disorder, or one or moresymptoms thereof. In accordance with these embodiments, the compositionmay further comprise of a carrier, diluent, or excipient.

The antibodies of the invention can be incorporated into pharmaceuticalcompositions suitable for administration to a subject. Typically, thepharmaceutical composition comprises an antibody of the invention and apharmaceutically acceptable carrier. As used herein, “pharmaceuticallyacceptable carrier” includes any and all solvents, dispersion media,coatings, antibacterial and antifungal agents, isotonic and absorptiondelaying agents, and the like that are physiologically compatible.Examples of pharmaceutically acceptable carriers include one or more ofwater, saline, phosphate buffered saline, dextrose, glycerol, ethanoland the like, as well as combinations thereof. In many cases, it will bepreferable to include isotonic agents, for example, sugars, polyalcoholssuch as mannitol, sorbitol, or sodium chloride in the composition.Pharmaceutically acceptable carriers may further comprise minor amountsof auxiliary substances such as wetting or emulsifying agents,preservatives, or buffers, which enhance the shelf life or effectivenessof the antibody.

Various delivery systems are known and can be used to administer one ormore antibodies of the invention or the combination of one or moreantibodies of the invention and a prophylactic agent or therapeuticagent useful for preventing, managing, treating, or ameliorating adisorder or one or more symptoms thereof, e.g., encapsulation inliposomes, microparticles, microcapsules, recombinant cells capable ofexpressing the antibody or antibody fragment, receptor-mediatedendocytosis (see, e.g., Wu and Wu, J. Biol. Chem. 262:4429-4432 (1987)),construction of a nucleic acid as part of a retroviral or other vector,etc. Methods of administering a prophylactic or therapeutic agent of theinvention include, but are not limited to, parenteral administration(e.g., intradermal, intramuscular, intraperitoneal, intravenous andsubcutaneous), epidurala administration, intratumoral administration,and mucosal administration (e.g., intranasal and oral routes). Inaddition, pulmonary administration can be employed, e.g., by use of aninhaler or nebulizer, and formulation with an aerosolizing agent. See,e.g., U.S. Pat. Nos. 6,019,968; 5,985,320; 5,985,309; 5,934,272;5,874,064; 5,855,913; 5,290,540; and 4,880,078; and PCT Publication Nos.WO 92/19244; WO97/32572; WO97/44013; WO98/31346; and WO99/66903, each ofwhich is incorporated herein by reference in their entireties. In oneembodiment, an antibody of the invention or a composition of theinvention is administered using Alkermes AIR® pulmonary drug deliverytechnology (Alkermes, Inc., Cambridge, Mass.). In a specific embodiment,prophylactic or therapeutic agents of the invention are administeredintramuscularly, intravenously, intratumorally, orally, intranasally,pulmonary, or subcutaneously. The prophylactic or therapeutic agents maybe administered by any convenient route, for example by infusion orbolus injection, by absorption through epithelial or mucocutaneouslinings (e.g., oral mucosa, rectal and intestinal mucosa, etc.) and maybe administered together with other biologically active agents.Administration can be systemic or local.

In a specific embodiment, it may be desirable to administer theantibodies of the invention locally to the area in need of treatment;this may be achieved by, for example, and not by way of limitation,local infusion, by injection, or by means of an implant, said implantbeing of a porous or non-porous material, including membranes andmatrices, such as sialastic membranes, polymers, fibrous matrices (e.g.,Tissuel®), or collagen matrices. In one embodiment, an effective amountof one or more antibodies of the invention is administered locally tothe affected area to a subject to prevent, treat, manage, and/orameliorate a disorder or a symptom thereof.

In another embodiment, the antibody can be delivered in a controlledrelease or sustained release system. In one embodiment, a pump may beused to achieve controlled or sustained release (see Langer, supra;Sefton, 1987, CRC Crit. Ref Biomed. Eng. 14:20; Buchwald et al., 1980,Surgery 88:507; Saudek et al., 1989, N. Engl. J. Med. 321:574). Inanother embodiment, polymeric materials can be used to achievecontrolled or sustained release of the therapies of the invention (seee.g., Medical Applications of Controlled Release, Langer and Wise(eds.), CRC Pres., Boca Raton, Fla. (1974); Controlled DrugBioavailability, Drug Product Design and Performance, Smolen and Ball(eds.), Wiley, New York (1984); Ranger and Peppas, 1983, J., Macromol.Sci. Rev. Macromol. Chem. 23:61; see also Levy et al., 1985, Science228:190; During et al., 1989, Ann. Neurol. 25:351; Howard et al., 1989,J. Neurosurg. 7 1:105); U.S. Pat. No. 5,679,377; U.S. Pat. No.5,916,597; U.S. Pat. No. 5,912,015; U.S. Pat. No. 5,989,463; U.S. Pat.No. 5,128,326; PCT Publication No. WO99/15154; and PCT Publication No.WO99/20253. Examples of polymers used in sustained release formulationsinclude, but are not limited to, poly(-hydroxy ethyl methacry-late),poly(methyl methacrylate), poly(acrylic acid), poly(ethylene-co-vinylacetate), poly(methacrylic acid), polyglycolides (PLG), polyanhydrides,poly(N-vinyl pyrrolidone), poly(vinyl alcohol), polyacrylamide,poly(ethylene glycol), polylactides (PLA), poly(lactide-co-glycolides)(PLGA), and polyorthoesters. In a particular embodiment, the polymerused in a sustained release formulation is inert, free of leachableimpurities, stable on storage, sterile, and biodegradable. In yetanother embodiment, a controlled or sustained release system can beplaced in proximity of the prophylactic or therapeutic target, thusrequiring only a fraction of the systemic dose (see, e.g., Goodson, inMedical Applications of Controlled Release, supra, vol. 2, pp. 115-138(1984)).

Controlled release systems are discussed in the review by Langer (1990,Science 249:1527-1533). Any technique known to one of skill in the artcan be used to produce sustained release formulations comprising one ormore antibodies of the invention. See, e.g., U.S. Pat. No. 4,526,938,PCT publication WO91/05548, PCT publication WO96/20698, Ning et al.,1996, “Intratumoral Radioimmunotheraphy of a Human Colon CancerXenograft Using a Sustained-Release Gel,” Radiotherapy &Oncology39:179-189; Song et al., 1995, “Antibody Mediated Lung Targeting ofLong-Circulating Emulsions,” PDA Journal of Pharmaceutical Science &Technology 50:372-397; Cleek et al., 1997, “Biodegradable PolymericCarriers for a bFGF Antibody for Cardiovascular Application,” Pro. Intl.Symp. Control. Rel. Bioact. Mater. 24:853-854; and Lam et al., 1997,“Microencapsulation of Recombinant Humanized Monoclonal Antibody forLocal Delivery,” Proc. Int'l. Symp. Control Rel. Bioact. Mater.24:759-760, each of which is incorporated herein by reference in theirentireties.

In a specific embodiment, where the composition of the invention is anucleic acid encoding an antibody, the nucleic acid can be administeredin vivo to promote expression of its encoded antibody, by constructingit as part of an appropriate nucleic acid expression vector andadministering it so that it becomes intracellular, e.g., by use of aretroviral vector (see U.S. Pat. No. 4,980,286), or by direct injection,or by use of microparticle bombardment (e.g., a gene gun; Biolistic,Dupont), or coating with lipids or cell-surface receptors ortransfecting agents, or by administering it in linkage to ahomeobox-like peptide which is known to enter the nucleus (see, e.g.,Joliot et al., 1991, Proc. Natl. Acad. Sci. USA 88:1864-1868).Alternatively, a nucleic acid can be introduced intracellularly andincorporated within host cell DNA for expression by homologousrecombination.

A pharmaceutical composition of the invention is formulated to becompatible with its intended route of administration. Examples of routesof administration include, but are not limited to, parenteral, e.g.,intravenous, intradermal, subcutaneous, oral, intranasal (e.g.,inhalation), transdermal (e.g., topical), transmucosal, and rectaladministration. In a specific embodiment, the composition is formulatedin accordance with routine procedures as a pharmaceutical compositionadapted for intravenous, subcutaneous, intramuscular, oral, intranasal,or topical administration to human beings. Typically, compositions forintravenous administration are solutions in sterile isotonic aqueousbuffer. Where necessary, the composition may also include a solubilizingagent and a local anesthetic such as lignocaine to ease pain at the siteof the injection.

If the compositions of the invention are to be administered topically,the compositions can be formulated in the form of an ointment, cream,transdermal patch, lotion, gel, shampoo, spray, aerosol, solution,emulsion, or other form well-known to one of skill in the art. See,e.g., Remington's Pharmaceutical Sciences and Introduction toPharmaceutical Dosage Forms, 19th ed., Mack Pub. Co., Easton, Pa.(1995). For non-sprayable topical dosage forms, viscous to semi-solid orsolid forms comprising a carrier or one or more excipients compatiblewith topical application and having a dynamic viscosity greater thanwater are typically employed. Suitable formulations include, withoutlimitation, solutions, suspensions, emulsions, creams, ointments,powders, liniments, salves, and the like, which are, if desired,sterilized or mixed with auxiliary agents (e.g., preservatives,stabilizers, wetting agents, buffers, or salts) for influencing variousproperties, such as, for example, osmotic pressure. Other suitabletopical dosage forms include sprayable aerosol preparations wherein theactive ingredient, for example in combination with a solid or liquidinert carrier, is packaged in a mixture with a pressurized volatile(e.g., a gaseous propellant, such as freon) or in a squeeze bottle.Moisturizers or humectants can also be added to pharmaceuticalcompositions and dosage forms if desired. Examples of such additionalingredients are well-known in the art.

If the method of the invention comprises intranasal administration of acomposition, the composition can be formulated in an aerosol form,spray, mist or in the form of drops. In particular, prophylactic ortherapeutic agents for use according to the present invention can beconveniently delivered in the form of an aerosol spray presentation frompressurized packs or a nebuliser, with the use of a suitable propellant(e.g., dichlorodifluoromethane, trichloro-fluoromethane,dichlorotetrafluoroethane, carbon dioxide or other suitable gas). In thecase of a pressurized aerosol the dosage unit may be determined byproviding a valve to deliver a metered amount. Capsules and cartridges(composed of, e.g., gelatin) for use in an inhaler or insufflator may beformulated containing a powder mix of the compound and a suitable powderbase such as lactose or starch.

If the method of the invention comprises oral administration,compositions can be formulated orally in the form of tablets, capsules,cachets, gelcaps, solutions, suspensions, and the like. Tablets orcapsules can be prepared by conventional means with pharmaceuticallyacceptable excipients such as binding agents (e.g., pregelatinised maizestarch, polyvinylpyrrolidone, or hydroxypropyl methylcellulose); fillers(e.g., lactose, microcrystalline cellulose, or calcium hydrogenphosphate); lubricants (e.g., magnesium stearate, talc, or silica);disintegrants (e.g., potato starch or sodium starch glycolate); orwetting agents (e.g., sodium lauryl sulphate). The tablets may be coatedby methods well-known in the art. Liquid preparations for oraladministration may take the form of, but not limited to, solutions,syrups or suspensions, or they may be presented as a dry product forconstitution with water or other suitable vehicle before use. Suchliquid preparations may be prepared by conventional means withpharmaceutically acceptable additives such as suspending agents (e.g.,sorbitol syrup, cellulose derivatives, or hydrogenated edible fats);emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles(e.g., almond oil, oily esters, ethyl alcohol, or fractionated vegetableoils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates orsorbic acid). The preparations may also contain buffer salts, flavoring,coloring, and sweetening agents as appropriate. Preparations for oraladministration may be suitably formulated for slow release, controlledrelease, or sustained release of a prophylactic or therapeutic agent(s).

The method of the invention may comprise pulmonary administration, e.g.,by use of an inhaler or nebulizer, of a composition formulated with anaerosolizing agent. See, e.g., U.S. Pat. Nos. 6,019,968; 5,985,320;5,985,309; 5,934,272; 5,874,064; 5,855,913; 5,290,540; and 4,880,078;and PCT Publication Nos. WO 92/19244; WO 97/32572; WO 97/44013; WO98/31346; and WO 99/66903, each of which is incorporated herein byreference their entireties. In a specific embodiment, an antibody of theinvention and/or composition of the invention is administered usingAlkermes AIR® pulmonary drug delivery technology (Alkermes, Inc.,Cambridge, Mass.).

The method of the invention may comprise administration of a compositionformulated for parenteral administration by injection (e.g., by bolusinjection or continuous infusion). Formulations for injection may bepresented in unit dosage form (e.g., in ampoules or in multi-dosecontainers) with an added preservative. The compositions may take suchforms as suspensions, solutions or emulsions in oily or aqueousvehicles, and may contain formulatory agents such as suspending,stabilizing and/or dispersing agents. Alternatively, the activeingredient may be in powder form for constitution with a suitablevehicle (e.g., sterile pyrogen-free water) before use. The methods ofthe invention may additionally comprise of administration ofcompositions formulated as depot preparations. Such long actingformulations may be administered by implantation (e.g., subcutaneouslyor intramuscularly) or by intramuscular injection. Thus, for example,the compositions may be formulated with suitable polymeric orhydrophobic materials (e.g., as an emulsion in an acceptable oil) or ionexchange resins, or as sparingly soluble derivatives (e.g., as asparingly soluble salt).

The methods of the invention encompass administration of compositionsformulated as neutral or salt forms. Pharmaceutically acceptable saltsinclude those formed with anions such as those derived fromhydrochloric, phosphoric, acetic, oxalic, tartaric acids, etc., andthose formed with cations such as those derived from sodium, potassium,ammonium, calcium, ferric hydroxides, isopropylamine, triethylamine,2-ethylamino ethanol, histidine, procaine, etc.

Generally, the ingredients of compositions are supplied eitherseparately or mixed together in unit dosage form, for example, as a drylyophilized powder or water free concentrate in a hermetically sealedcontainer such as an ampoule or sachette indicating the quantity ofactive agent. Where the mode of administration is infusion, compositioncan be dispensed with an infusion bottle containing sterilepharmaceutical grade water or saline. Where the mode of administrationis by injection, an ampoule of sterile water for injection or saline canbe provided so that the ingredients may be mixed prior toadministration.

In particular, the invention also provides that one or more of theantibodies, or pharmaceutical compositions, of the invention is packagedin a hermetically sealed container such as an ampoule or sachetteindicating the quantity of the antibody. In one embodiment, one or moreof the antibodies, or pharmaceutical compositions of the invention issupplied as a dry sterilized lyophilized powder or water freeconcentrate in a hermetically sealed container and can be reconstituted(e.g., with water or saline) to the appropriate concentration foradministration to a subject. In one embodiment, one or more of theantibodies or pharmaceutical compositions of the invention is suppliedas a dry sterile lyophilized powder in a hermetically sealed containerat a unit dosage of at least 5 mg, for example at least 10 mg, at least15 mg, at least 25 mg, at least 35 mg, at least 45 mg, at least 50 mg,at least 75 mg, or at least 100 mg. The lyophilized antibodies orpharmaceutical compositions of the invention should be stored at between2° C. and 8° C. in its original container and the antibodies, orpharmaceutical compositions of the invention should be administeredwithin 1 week, for example within 5 days, within 72 hours, within 48hours, within 24 hours, within 12 hours, within 6 hours, within 5 hours,within 3 hours, or within 1 hour after being reconstituted. In analternative embodiment, one or more of the antibodies or pharmaceuticalcompositions of the invention is supplied in liquid form in ahermetically sealed container indicating the quantity and concentrationof the antibody. In a further embodiment, the liquid form of theadministered composition is supplied in a hermetically sealed containerat least 0.25 mg/ml, for example at least 0.5 mg/ml, at least 1 mg/ml,at least 2.5 mg/ml, at least 5 mg/ml, at least 8 mg/ml, at least 10mg/ml, at least 15 mg/ml, at least 25 mg/ml, at least 50 mg/ml, at least75 mg/ml or at least 100 mg/ml. The liquid form should be stored atbetween 2° C. and 8° C. in its original container.

The antibodies of the invention can be incorporated into apharmaceutical composition suitable for parenteral administration. Inone aspect, antibodies will be prepared as an injectable solutioncontaining 0.1-250 mg/ml antibody. The injectable solution can becomposed of either a liquid or lyophilized dosage form in a flint oramber vial, ampule or pre-filled syringe. The buffer can be L-histidine(1-50 mM), optimally 5-10 mM, at pH 5.0 to 7.0 (optimally pH 6.0). Othersuitable buffers include but are not limited to, sodium succinate,sodium citrate, sodium phosphate or potassium phosphate. Sodium chloridecan be used to modify the tonicity of the solution at a concentration of0-300 mM (optimally 150 mM for a liquid dosage form). Cryoprotectantscan be included for a lyophilized dosage form, principally 0-10% sucrose(optimally 0.5-1.0%). Other suitable cryoprotectants include trehaloseand lactose. Bulking agents can be included for a lyophilized dosageform, principally 1-10% mannitol (optimally 2-4%). Stabilizers can beused in both liquid and lyophilized dosage forms, principally 1-50 mML-Methionine (optimally 5-10 mM). Other suitable bulking agents includeglycine, arginine, can be included as 0-0.05% polysorbate-80 (optimally0.005-0.01%). Additional surfactants include but are not limited topolysorbate 20 and BRIJ surfactants. The pharmaceutical compositioncomprising the antibodies of the invention prepared as an injectablesolution for parenteral administration, can further comprise an agentuseful as an adjuvant, such as those used to increase the absorption, ordispersion of the antibody. A particularly useful adjuvant ishyaluronidase, such as Hylenex® (recombinant human hyaluronidase).Addition of hyaluronidase in the injectable solution improves humanbioavailability following parenteral administration, particularlysubcutaneous administration. It also allows for greater injection sitevolumes (i.e. greater than 1 ml) with less pain and discomfort, andminimum incidence of injection site reactions. (See International Appln.Publication No. WO 04/078140 and U.S. Patent Appln. Publication No.US2006104968, incorporated herein by reference.)

The compositions of this invention may be in a variety of forms. Theseinclude, for example, liquid, semi-solid and solid dosage forms, such asliquid solutions (e.g., injectable and infusible solutions), dispersionsor suspensions, tablets, pills, powders, liposomes and suppositories.The preferred form depends on the intended mode of administration andtherapeutic application. Compositions can be in the form of injectableor infusible solutions, such as compositions similar to those used forpassive immunization of humans with other antibodies. In one embodiment,the antibody is administered by intravenous infusion or injection. Inanother embodiment, the antibody is administered by intramuscular orsubcutaneous injection.

Therapeutic compositions typically must be sterile and stable under theconditions of manufacture and storage. The composition can be formulatedas a solution, microemulsion, dispersion, liposome, or other orderedstructure suitable to high drug concentration. Sterile injectablesolutions can be prepared by incorporating the active compound (i.e., abinding protein, e.g. an antibody, of the present invention) in therequired amount in an appropriate solvent with one or a combination ofingredients enumerated above, as required, followed by filteredsterilization. Generally, dispersions are prepared by incorporating theactive compound into a sterile vehicle that contains a basic dispersionmedium and the required other ingredients from those enumerated above.In the case of sterile, lyophilized powders for the preparation ofsterile injectable solutions, methods of preparation comprise vacuumdrying and spray-drying that yields a powder of the active ingredientplus any additional desired ingredient from a previouslysterile-filtered solution thereof. The proper fluidity of a solution canbe maintained, for example, by the use of a coating such as lecithin, bythe maintenance of the required particle size in the case of dispersionand by the use of surfactants. Prolonged absorption of injectablecompositions can be brought about by including, in the composition, anagent that delays absorption, for example, monostearate salts andgelatin.

The antibodies of the present invention can be administered by a varietyof methods known in the art. For many therapeutic applications, theroute/mode of administration may be subcutaneous injection, intravenousinjection or infusion. As will be appreciated by the skilled artisan,the route and/or mode of administration will vary depending upon thedesired results. In certain embodiments, the active compound may beprepared with a carrier that will protect the compound against rapidrelease, such as a controlled release formulation, including implants,transdermal patches, and microencapsulated delivery systems.Biodegradable, biocompatible polymers can be used, such as ethylenevinyl acetate, polyanhydrides, polyglycolic acid, collagen,polyorthoesters, and polylactic acid. Many methods for the preparationof such formulations are patented or generally known to those skilled inthe art. See, e.g., Sustained and Controlled Release Drug DeliverySystems, J. R. Robinson, ed., Marcel Dekker, Inc., New York, 1978.

In certain embodiments, an antibody of the invention may be orallyadministered, for example, with an inert diluent or an assimilableedible carrier. The antibody (and other ingredients, if desired) mayalso be enclosed in a hard or soft shell gelatin capsule, compressedinto tablets, or incorporated directly into the subject's diet. For oraltherapeutic administration, the antibody may be incorporated withexcipients and used in the form of ingestible tablets, buccal tablets,troches, capsules, elixirs, suspensions, syrups, wafers, and the like.To administer an antibody of the invention by other than parenteraladministration, it may be necessary to coat the antibody with, orco-administer the antibody with, a material to prevent its inactivation.

In certain embodiments, an antibody of the invention is linked to ahalf-life extending vehicle known in the art. Such vehicles include, butare not limited to, the Fc domain, polyethylene glycol, and dextran.Such vehicles are described, e.g., in U.S. application Ser. No.09/428,082 and published PCT Application No. WO 99/25044, which arehereby incorporated by reference for any purpose.

In a specific embodiment, nucleic acid sequences comprising nucleotidesequences encoding an antibody of the invention are administered totreat, prevent, manage, or ameliorate a disorder or one or more symptomsthereof by way of gene therapy. Gene therapy refers to therapy performedby the administration to a subject of an expressed or expressiblenucleic acid. In this embodiment of the invention, the nucleic acidsproduce their encoded antibody of the invention that mediates aprophylactic or therapeutic effect.

Any of the methods for gene therapy available in the art can be usedaccording to the present invention. For general reviews of the methodsof gene therapy, see Goldspiel et al., 1993, Clinical Pharmacy12:488-505; Wu and Wu, 1991, Biotherapy 3:87-95; Tolstoshev, 1993, Ann.Rev. Pharmacol. Toxicol. 32:573-596; Mulligan, Science 260:926-932(1993); and Morgan and Anderson, 1993, Ann. Rev. Biochem. 62:191-217;May, 1993, TIBTECH 11(5):155-215. Methods commonly known in the art ofrecombinant DNA technology which can be used are described in Ausubel etal. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons,NY (1993); and Kriegler, Gene Transfer and Expression, A LaboratoryManual, Stockton Press, NY (1990). Detailed description of variousmethods of gene therapy are disclosed in US20050042664 A1 which isincorporated herein by reference.

Antibodies of the invention can be used alone or in combination to treatdiseases or conditions associated with liver disease and/or cancers, orany other disease or condition associated with GP73. It should furtherbe understood that the combinations are those combinations useful fortheir intended purpose.

The pharmaceutical compositions may include a “therapeutically effectiveamount” or a “prophylactically effective amount” of an antibody. A“therapeutically effective amount” refers to an amount effective, atdosages and for periods of time necessary, to achieve the desiredtherapeutic result. A therapeutically effective amount of the antibodymay be determined by a person skilled in the art and may vary accordingto factors such as the disease state, age, sex, and weight of theindividual, and the ability of the antibody to elicit a desired responsein the individual. A therapeutically effective amount is also one inwhich toxic or detrimental effects, if any, of the antibody areoutweighed by the therapeutically beneficial effects. A“prophylactically effective amount” refers to an amount effective, atdosages and for periods of time necessary, to achieve the desiredprophylactic result. Typically, since a prophylactic dose is used insubjects prior to or at an earlier stage of disease, theprophylactically effective amount will be less than the therapeuticallyeffective amount.

Dosage regimens may be adjusted to provide the optimum desired response(e.g., a therapeutic or prophylactic response). For example, a singlebolus may be administered, several divided doses may be administeredover time or the dose may be proportionally reduced or increased asindicated by the exigencies of the therapeutic situation. It isespecially advantageous to formulate parenteral compositions in dosageunit form for ease of administration and uniformity of dosage. Dosageunit form as used herein refers to physically discrete units suited asunitary dosages for the mammalian subjects to be treated; each unitcontaining a predetermined quantity of active compound calculated toproduce the desired therapeutic effect in association with the requiredpharmaceutical carrier. The specification for the dosage unit forms aredictated by and directly dependent on (a) the unique characteristics ofthe active compound and the particular therapeutic or prophylacticeffect to be achieved, and (b) the limitations inherent in the art ofcompounding such an active compound for the treatment of sensitivity inindividuals.

An exemplary, non-limiting range for a therapeutically orprophylactically effective amount of the antibody is a dose of between0.1 and 200 mg/kg, for example between 0.1 and 10 mg/kg. Thetherapeutically or prophylactically effective amount of the antibody maybe between 1 and 200 mg/kg, 10 and 200 mg/kg, 20 and 200 mg/kg, 50 and200 mg/kg, 75 and 200 mg/kg, 100 and 200 mg/kg, 150 and 200 mg/kg, 50and 100 mg/kg, 5 and 10 mg/kg, or 1 and 10 mg/kg. It is to be noted thatdosage values may vary with the type and severity of the condition to bealleviated. Further, the antibody dose may be determined by a personskilled in the art and may vary according to factors such as the diseasestate, age, sex, and weight of the individual, and the ability of theantibody to elicit a desired response in the individual. The dose isalso one in which toxic or detrimental effects, if any, of the antibodyare outweighed by the therapeutically beneficial effects. It is to befurther understood that for any particular subject, specific dosageregimens should be adjusted over time according to the individual needand the professional judgment of the person administering or supervisingthe administration of the compositions, and that dosage ranges set forthherein are exemplary only and are not intended to limit the scope orpractice of the claimed composition.

4. GP73 DETECTION

The present invention also is directed to a method of detecting andmeasuring GP73 or fucosylated GP73 in a sample from a subject using theGP73 antibodies described above to bind to different GP73 or fucosylatedGP73 epitopes. The method includes (a) obtaining a biological samplefrom a subject, (b) contacting the biological sample with a captureantibody, which binds to an epitope on GP73 (or GP73 fragment) orfucosylated GP73 (or fucosylated GP73 fragment) to form a captureantibody-GP73 antigen complex, (c) contacting the capture antibody—GP73or or fucosylated GP73 antigen complex with a detection antibody whichincludes a detectable label and binds to an epitope on GP73 or orfucosylated GP73 that is not bound by the capture antibody, to form acapture antibody-GP73 or fucosylated GP73 antigen-detection antibody,and (d) determining the presence, amount or concentration of GP73 or orfucosylated GP73 in the biological sample based on the signal generatedby the detectable label in the capture antibody-GP73 or fucosylated GP73antigen-detection antibody complex.

The present invention is further directed to a method of detecting andmeasuring fucosylated GP73 or fucosylated GP73 in a sample from asubject using the GP73 antibodies describe above. The binding of theGP73 antibodies may or may not be sensitive to fucosylation of GP73. Themethod includes (a) contacting the test sample with at least one capturebinding protein, wherein the capture binding protein binds to a regionof GP73 (or a fragment of GP73) or or fucosylated GP73 (or a fragment offucosylated GP73) to form a capture binding protein-GP73 or fucosylatedGP73 complex; (b) contacting the capture binding protein-GP73 orfucosylated GP73 complex with at least one detection binding proteincomprising a detectable label, wherein the detection binding proteinbinds to a region of GP73 or fucosylated GP73 that is not bound by thecapture binding protein and forms a capture binding protein-GP73 orfucosylated GP73-detection binding protein complex; and (c) determiningthe GP73 or fucosylated GP73 concentration in the test sample based onthe signal generated by the detectable label in the capture bindingprotein-GP73 or fucosylated GP73-detection binding protein complexformed in (b). The capture binding protein comprises a protein, antibodyor antibody fragment whose binding to GP73 or fucosylated GP73 issensitive to the presence or absence of a fucose sugar moiety on GP73 orfucosylated GP73 and the detection binding protein comprises a protein,antibody or antibody fragment whose binding to GP73 or fucosylated GP73is insensitive to the presence or absence of a fucose sugar moiety onGP73 or fucosylated GP73. Alternatively, the capture binding proteincomprises a protein, antibody or antibody fragment whose binding to GP73or fucosylated GP73 is insensitive to the presence or absence of afucose sugar moiety on GP73 or fucosylated GP73 and the detectionbinding protein comprises a protein, antibody or antibody fragment whosebinding to GP73 or fucosylated GP73 is sensitive to the presence orabsence of a fucose sugar moiety on GP73 or fucosylated GP73.

The present invention is further directed to a method for diagnosing adisease in a subject based on the GP73 (or GP73 fragment) levels orfucosylated GP73 (or fucosylated GP73 fragment) levels in a sample fromthe subject. The method includes the steps of (a) obtaining a biologicalsample from a subject, (b) determining the level of GP73 or fucosylatedGP73 in the biological sample, (c) comparing the level of GP73 orfucosylated GP73 in the biological sample to a reference level of GP73or fucosylated GP73, (d) identifying the subject as having a disease ifthe level of GP73 or fucosylated GP73 in the biological sample isgreater than the reference level of GP73 or fucosylated GP73, and (e)administering a treatment regimen to the subject identified as havingdisease.

Levels of at least 0.05 ng/mL, 0.06 ng/mL, 0.07 ng/mL, 0.08 ng/mL, 0.09ng/mL, 0.10 ng/mL, 0.11 ng/mL, 0.12 ng/mL, 0.13 ng/mL, 0.14 ng/mL, 0.15ng/mL, 0.16 ng/mL, 0.17 ng/mL, 0.18 ng/mL, 0.19 ng/mL, 0.20 ng/mL, 0.25ng/mL, 0.30 ng/mL, 0.35 ng/mL, 0.40 ng/mL, 0.45 ng/mL, 0.50 ng/mL, 0.55ng/mL, 1 ng/mL, 5 ng/mL, 10 ng/mL, 15 ng/mL, 20 ng/mL, 21 ng/mL, 22ng/mL, 23 ng/mL, 24 ng/mL, 25 ng/mL, 26 ng/mL, 27 ng/mL, 28 ng/mL, 29ng/mL, or 30 ng/mL of GP73 (or GP73 fragment) or fucosylated GP73 (orfucosylated GP73 fragment) in a biological sample may be detected.

Ranges of GP73 and fucosylated GP73 detection have at least 5%, 10%,25%, 50%, 75%, 100%, 110%, 120%, 130%, 140%, 150%, 160%, 170%, 180%,190%, 200%, 210%, 220%, 230%, 240%, 250%, 260%, 270%, 280%, 290%, 300%,400%, or 500% improved range size compared to other commerciallyavailable GP73 or fucosylated GP73 immunoassays.

Ranges of about 0 ng/mL to about 30 ng/mL, about 0.05 ng/mL to about 30ng/mL, about 0.06 ng/mL to about 30 ng/mL, about 0.07 ng/mL to about 30ng/mL, about 0.08 ng/mL to about 30 ng/mL, about 0.09 ng/mL to about 30ng/mL, about 0.095 ng/mL to about 30 ng/mL, about 0.10 ng/mL to about 30ng/mL, about 0.105 ng/mL to about 30 ng/mL, about 0.11 ng/mL to about 30ng/mL, about 0.12 ng/mL to about 30 ng/mL, about 0.13 ng/mL to about 30ng/mL, about 0.14 ng/mL to about 30 ng/mL, about 0.15 ng/mL to about 30ng/mL, about 0.20 ng/mL to about 30 ng/mL, about 1.00 ng/mL to about 30,about 0 ng/mL to about 27.5 ng/mL, 0.05 ng/mL to about 27.5 ng/mL, 0.06ng/mL to about 27.5 ng/mL, 0.07 ng/mL to about 27.5 ng/mL, 0.08 ng/mL toabout 27.5 ng/mL, 0.09 ng/mL to about 27.5 ng/mL, 0.095 ng/mL to about27.5 ng/mL, 0.10 ng/mL to about 27.5 ng/mL, 0.105 ng/mL to about 27.5ng/mL, 0.11 ng/mL to about 27.5 ng/mL, 0.12 ng/mL to about 27.5 ng/mL,0.13 ng/mL to about 27.5 ng/mL, 0.14 ng/mL to about 27.5 ng/mL, 0.15ng/mL to about 27.5 ng/mL, 0.20 ng/mL to about 27.5 ng/mL, 1.00 ng/mL toabout 27.5 ng/mL, about 0 ng/mL to about 26 ng/mL, about 0.05 ng/mL toabout 26 ng/mL, 0.06 ng/mL to about 26 ng/mL, 0.07 ng/mL to about 26ng/mL, 0.08 ng/mL to about 26 ng/mL, 0.09 ng/mL to about 26 ng/mL, 0.095ng/mL to about 26 ng/mL, 0.10 ng/mL to about 26 ng/mL, 0.105 ng/mL toabout 26 ng/mL, 0.11 ng/mL to about 26 ng/mL, 0.12 ng/mL to about 26ng/mL, 0.13 ng/mL to about 26 ng/mL, 0.14 ng/mL to about 26 ng/mL, 0.15ng/mL to about 26 ng/mL, 0.20 ng/mL to about 26 ng/mL, 1.00 ng/mL toabout 26 ng/mL, about 0 ng/mL to about 25 ng/mL, 0.05 ng/mL to about 25ng/mL, 0.06 ng/mL to about 25 ng/mL, 0.07 ng/mL to about 25 ng/mL, 0.08ng/mL to about 25 ng/mL, 0.09 ng/mL to about 25 ng/mL, 0.095 ng/mL toabout 25 ng/mL, 0.10 ng/mL to about 25 ng/mL, 0.105 ng/mL to about 25ng/mL, 0.11 ng/mL to about 25 ng/mL, 0.12 ng/mL to about 25 ng/mL, 0.13ng/mL to about 25 ng/mL, 0.14 ng/mL to about 25 ng/mL, 0.15 ng/mL toabout 25 ng/mL, 0.20 ng/mL to about 25 ng/mL, 1.00 ng/mL to about 25ng/mL, about 0 ng/mL to about 24 ng/mL, 0.05 ng/mL to about 24 ng/mL,0.06 ng/mL to about 24 ng/mL, 0.07 ng/mL to about 24 ng/mL, 0.08 ng/mLto about 24 ng/mL, 0.09 ng/mL to about 24 ng/mL, 0.095 ng/mL to about 24ng/mL, 0.10 ng/mL to about 24 ng/mL, 0.105 ng/mL to about 24 ng/mL, 0.11ng/mL to about 24 ng/mL, 0.12 ng/mL to about 24 ng/mL, 0.13 ng/mL toabout 24 ng/mL, 0.14 ng/mL to about 24 ng/mL, 0.15 ng/mL to about 24ng/mL, 0.20 ng/mL to about 24 ng/mL, 1.00 ng/mL to about 24 ng/mL, about0 ng/mL to about 22.5 ng/mL, 0.05 ng/mL to about 22.5 ng/mL, 0.06 ng/mLto about 22.5 ng/mL, 0.07 ng/mL to about 22.5 ng/mL, 0.08 ng/mL to about22.5 ng/mL, 0.09 ng/mL to about 22.5 ng/mL, 0.095 0 ng/mL to about 22.5ng/mL, 0.10 ng/mL to about 22.5 ng/mL, 0.105 ng/mL to about 22.5 ng/mL,0.11 ng/mL to about 22.5 ng/mL, 0.12 ng/mL to about 22.5 ng/mL, 0.13ng/mL to about 22.5 ng/mL, 0.14 ng/mL to about 22.5 ng/mL, 0.15 ng/mL toabout 22.5 ng/mL, 0.20 ng/mL to about 22.5 ng/mL, 1.00 ng/mL to about22.5 ng/Ml, about 0 ng/mL to about 20 ng/mL, 0.05 ng/mL to about 20ng/mL, 0.06 ng/mL to about 20 ng/mL, 0.07 ng/mL to about 20 ng/mL, 0.08ng/mL to about 20 ng/mL, 0.09 ng/mL to about 20 ng/mL, 0.095 ng/mL toabout 20 ng/mL, 0.10 ng/mL to about 20 ng/mL, 0.105 ng/mL to about 20ng/mL, 0.11 ng/mL to about 20 ng/mL, 0.12 ng/mL to about 20 ng/mL, 0.13ng/mL to about 20 ng/mL, 0.14 ng/mL to about 20 ng/mL, 0.15 ng/mL toabout 20 ng/mL, 0.20 ng/mL to about 20 ng/mL, 1.00 ng/mL to about 20ng/mL, about 0 ng/mL to about 15 ng/mL, 0.05 ng/mL to about 15 ng/mL,0.06 ng/mL to about 15 ng/mL, 0.07 ng/mL to about 15 ng/mL, 0.08 ng/mLto about 15 ng/mL, 0.09 ng/mL to about 15 ng/mL, 0.095 ng/mL to about 15ng/mL, 0.10 ng/mL to about 15 ng/mL, 0.105 ng/mL to about 15 ng/mL, 0.11ng/mL to about 15 ng/mL, 0.12 ng/mL to about 15 ng/mL, 0.13 ng/mL toabout 15 ng/mL, 0.14 ng/mL to about 15 ng/mL, 0.15 ng/mL to about 15ng/mL, 0.20 ng/mL to about 15 ng/mL, or 1.00 ng/mL to about 15 ng/mL ofGP73 or fucosylated GP73 may be detected.

a. Immunoassay

GP73 and fucosylated GP73, and/or peptides or fragments thereof, i.e.,GP73 and fucosylated GP73 fragments, may be analyzed using theantibodies described above in an immunoassay. The presence or amount ofGP73 or fucosylated GP73 can be determined using antibodies anddetecting specific binding to GP73 or fucosylated GP73. For example, theantibody, or antibody fragment thereof, may specifically bind to GP73 orfucosylated GP73. If desired, one or more of the antibodies can be usedin combination with one or more commercially availablemonoclonal/polyclonal antibodies. Such antibodies are available fromcompanies such as R&D Systems, Inc. (Minneapolis, Minn.) and Enzo LifeSciences International, Inc. (Plymouth Meeting, Pa.).

The presence or amount of GP73 or fucosylated GP73 present in a bodysample may be readily determined using an immunoassay, such as sandwichimmunoassay (e.g., monoclonal-polyclonal sandwich immunoassays,including radioisotope detection (radioimmunoassay (RIA)) and enzymedetection (enzyme immunoassay (EIA) or enzyme-linked immunosorbent assay(ELISA) (e.g., Quantikine ELISA assays, R&D Systems, Minneapolis,Minn.)). A chemiluminescent microparticle immunoassay, in particular oneemploying the ARCHITECTS automated analyzer (Abbott Laboratories, AbbottPark, Ill.), is an example of a preferred immunoassay. Other methodsinclude, for example, mass spectrometry and immunohistochemistry (e.g.with sections from tissue biopsies) using GP73 antibodies (monoclonal,polyclonal, chimeric, humanized, human etc) or antibody fragmentsthereof against GP73. Other methods of detection include those describedin, for example, U.S. Pat. Nos. 6,143,576; 6,113,855; 6,019,944;5,985,579; 5,947,124; 5,939,272; 5,922,615; 5,885,527; 5,851,776;5,824,799; 5,679,526; 5,525,524; and 5,480,792, each of which is herebyincorporated by reference in its entirety. Specific immunologicalbinding of the antibody to the GP73 can be detected via direct labels,such as fluorescent or luminescent tags, metals and radionuclidesattached to the antibody or via indirect labels, such as alkalinephosphatase or horseradish peroxidase.

The use of immobilized antibodies or antibody fragments thereof may beincorporated into the immunoassay. The antibodies may be immobilizedonto a variety of supports, such as magnetic or chromatographic matrixparticles, the surface of an assay plate (such as microtiter wells),pieces of a solid substrate material, and the like. An assay strip canbe prepared by coating the antibody or plurality of antibodies in anarray on a solid support. This strip can then be dipped into the testbiological sample and processed quickly through washes and detectionsteps to generate a measurable signal, such as a colored spot.

A heterogeneous format may be used. For example, after the test sampleis obtained from a subject, a first mixture is prepared. The mixturecontains the test sample being assessed for GP73 or or fucosylated GP73and a first specific binding partner, wherein the first specific bindingpartner and any GP73 or fucosylated GP73 contained in the test sampleform a first specific binding partner-GP73 or fucosylated GP73 antigencomplex. The first specific binding partner may be an anti-GP73 antibodythat binds to an epitope having an amino acid sequence comprising atleast three contiguous (3) amino acids of SEQ ID NO: 97. The order inwhich the test sample and the first specific binding partner are addedto form the mixture is not critical. The first specific binding partnermay be immobilized on a solid phase. The solid phase used in theimmunoassay (for the first specific binding partner and, optionally, thesecond specific binding partner) can be any solid phase known in theart, such as, but not limited to, a magnetic particle, a bead, a testtube, a microtiter plate, a cuvette, a membrane, a scaffolding molecule,a film, a filter paper, a disc, and a chip.

After the mixture containing the first specific binding partner-GP73 orfucosylated GP73 antigen complex is formed, any unbound GP73 orfucosylated GP73 is removed from the complex using any technique knownin the art. For example, the unbound GP73 or fucosylated GP73 can beremoved by washing. Desirably, however, the first specific bindingpartner is present in excess of any GP73 or fucosylated GP73 present inthe test sample, such that all GP73 or fucosylated GP73 that is presentin the test sample is bound by the first specific binding partner.

After any unbound GP73 or fucosylated GP73 is removed, a second specificbinding partner is added to the mixture to form a first specific bindingpartner-GP73 or fucosylated GP73 antigen-second specific binding partnercomplex. The second specific binding partner may be an anti-GP73 orfucosylated GP73 antibody that binds to an epitope having an amino acidsequence comprising at least three contiguous (3) amino acids of SEQ IDNO: 33. Moreover, the second specific binding partner is labeled with orcontains a detectable label as described above.

The use of immobilized antibodies or antibody fragments thereof may beincorporated into the immunoassay. The antibodies may be immobilizedonto a variety of supports, such as magnetic or chromatographic matrixparticles, the surface of an assay plate (such as microtiter wells),pieces of a solid substrate material, and the like. An assay strip canbe prepared by coating the antibody or plurality of antibodies in anarray on a solid support. This strip can then be dipped into the testbiological sample and processed quickly through washes and detectionsteps to generate a measurable signal, such as a colored spot.

(1) Sandwich ELISA

The Sandwich ELISA measures the amount of antigen between two layers ofantibodies (i.e., at least one capture antibody) and a detectionantibody (i.e. at least one detection antibody). The capture antibodyand the detection antibody bind to different epitopes on the antigen,e.g., GP73 or fucosylated GP73. Desirably, binding of the captureantibody to an epitope does not interfere with binding of the detectionantibody to an epitope. Either monoclonal or polyclonal antibodies maybe used as the capture and detection antibodies in the sandwich ELISA.

Generally, at least two antibodies are employed to separate and quantifyGP73 or or fucosylated GP73 in a test sample. More specifically, the atleast two antibodies bind to certain epitopes of GP73 or fucosylatedGP73 forming an immune complex which is referred to as a “sandwich”. Oneor more antibodies can be used to capture the GP73 or or fucosylatedGP73 in the test sample (these antibodies are frequently referred to asa “capture” antibody or “capture” antibodies) and one or more antibodiesis used to bind a detectable (namely, quantifiable) label to thesandwich (these antibodies are frequently referred to as the “detection”antibody or “detection” antibodies). In a sandwich assay, the binding ofan antibody to its epitope desirably is not diminished by the binding ofany other antibody in the assay to its respective epitope. Antibodiesare selected so that the one or more first antibodies brought intocontact with a test sample suspected of containing GP73 or fucosylatedGP73 do not bind to all or part of an epitope recognized by the secondor subsequent antibodies, thereby interfering with the ability of theone or more second detection antibodies to bind to the GP73 orfucosylated GP73.

The antibodies may be used as a first antibody in said immunoassay. Theantibody immunospecifically binds to epitopes on GP73 or fucosylatedGP73. In addition to the antibodies of the present invention, saidimmunoassay may comprise a second antibody that immunospecifically bindsto epitopes that are not recognized or bound by the first antibody.

A test sample suspected of containing GP73 or fucosylated GP73 can becontacted with at least one first capture antibody (or antibodies) andat least one second detection antibodies either simultaneously orsequentially. In the sandwich assay format, a test sample suspected ofcontaining GP73 or fucosylated GP73 is first brought into contact withthe at least one first capture antibody that specifically binds to aparticular epitope under conditions which allow the formation of a firstantibody-GP73 or fucosylated GP73 antigen complex. If more than onecapture antibody is used, a first multiple capture antibody-GP73 orfucosylated GP73 antigen complex is formed. In a sandwich assay, theantibodies, preferably, the at least one capture antibody, are used inmolar excess amounts of the maximum amount of GP73 or fucosylated GP73expected in the test sample. For example, from about 5 μg/ml to about 1mg/ml of antibody per ml of microparticle coating buffer may be used.

(a) Anti-GP73 Capture Antibody

Optionally, prior to contacting the test sample with the at least onefirst capture antibody, the at least one first capture antibody can bebound to a solid support which facilitates the separation the firstantibody-GP73 or fucosylated GP73 antigen complex from the test sample.Any solid support known in the art can be used, including but notlimited to, solid supports made out of polymeric materials in the formsof wells, tubes, or beads. The antibody (or antibodies) can be bound tothe solid support by adsorption, by covalent bonding using a chemicalcoupling agent or by other means known in the art, provided that suchbinding does not interfere with the ability of the antibody to bind GP73or fucosylated GP73. Moreover, if necessary, the solid support can bederivatized to allow reactivity with various functional groups on theantibody. Such derivatization requires the use of certain couplingagents such as, but not limited to, maleic anhydride,N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.

After the test sample suspected of containing GP73 or GP73 orfucosylated GP73 is incubated in order to allow for the formation of afirst capture antibody (or multiple antibody)-GP73 or fucosylated GP73antigen complex. The incubation can be carried out at a pH of from about4.5 to about 10.0, at a temperature of from about 2° C. to about 45° C.,and for a period from at least about one (1) minute to about eighteen(18) hours, from about 2-6 minutes, or from about 3-4 minutes.

(b) Detection Antibody

After formation of the first/multiple capture antibody-GP73 orfucosylated GP73 antigen complex, the complex is then contacted with atleast one second detection antibody (under conditions that allow for theformation of a first/multiple antibody-GP73 or fucosylated GP73antigen-second antibody complex). If the first antibody-GP73 orfucosylated GP73 or fucosylated GP73 antigen complex is contacted withmore than one detection antibody, then a first/multiple captureantibody-GP73 or fucosylated GP73 antigen-multiple antibody detectioncomplex is formed. As with first antibody, when the at least second (andsubsequent) antibody is brought into contact with the firstantibody-GP73 or fucosylated GP73 antigen complex, a period ofincubation under conditions similar to those described above is requiredfor the formation of the first/multiple antibody-GP73 or fucosylatedGP73 antigen-second/multiple antibody complex. Preferably, at least onesecond antibody contains a detectable label. The detectable label can bebound to the at least one second antibody prior to, simultaneously withor after the formation of the first/multiple antibody-GP73 orfucosylated GP73 antigen-second/multiple antibody complex. Anydetectable label known in the art can be used.

Chemiluminescent assays can be performed in accordance with the methodsdescribed in Adamczyk et al., Anal. Chim. Acta 579(1): 61-67 (2006).While any suitable assay format can be used, a microplatechemiluminometer (Mithras LB-940, Berthold Technologies U.S.A., LLC, OakRidge, Tenn.) enables the assay of multiple samples of small volumesrapidly. The chemiluminometer can be equipped with multiple reagentinjectors using 96-well black polystyrene microplates (Costar #3792).Each sample can be added into a separate well, followed by thesimultaneous/sequential addition of other reagents as determined by thetype of assay employed. Desirably, the formation of pseudobases inneutral or basic solutions employing an acridinium aryl ester isavoided, such as by acidification. The chemiluminescent response is thenrecorded well-by-well. In this regard, the time for recording thechemiluminescent response will depend, in part, on the delay between theaddition of the reagents and the particular acridinium employed.

The order in which the test sample and the specific binding partner(s)are added to form the mixture for chemiluminescent assay is notcritical. If the first specific binding partner is detectably labeledwith an acridinium compound, detectably labeled first specific bindingpartner-GP73 or fucosylated GP73 antigen complexes form. Alternatively,if a second specific binding partner is used and the second specificbinding partner is detectably labeled with an acridinium compound,detectably labeled first specific binding partner-GP73 or fucosylatedGP73 antigen-second specific binding partner complexes form. Any unboundspecific binding partner, whether labeled or unlabeled, can be removedfrom the mixture using any technique known in the art, such as washing.

Hydrogen peroxide can be generated in situ in the mixture or provided orsupplied to the mixture before, simultaneously with, or after theaddition of an above-described acridinium compound. Hydrogen peroxidecan be generated in situ in a number of ways such as would be apparentto one skilled in the art.

Alternatively, a source of hydrogen peroxide can be simply added to themixture. For example, the source of the hydrogen peroxide can be one ormore buffers or other solutions that are known to contain hydrogenperoxide. In this regard, a solution of hydrogen peroxide can simply beadded.

Upon the simultaneous or subsequent addition of at least one basicsolution to the sample, a detectable signal, namely, a chemiluminescentsignal, indicative of the presence of GP73 or fucosylated GP73 isgenerated. The basic solution contains at least one base and has a pHgreater than or equal to 10, preferably, greater than or equal to 12.Examples of basic solutions include, but are not limited to, sodiumhydroxide, potassium hydroxide, calcium hydroxide, ammonium hydroxide,magnesium hydroxide, sodium carbonate, sodium bicarbonate, calciumhydroxide, calcium carbonate, and calcium bicarbonate. The amount ofbasic solution added to the sample depends on the concentration of thebasic solution. Based on the concentration of the basic solution used,one skilled in the art can easily determine the amount of basic solutionto add to the sample.

The chemiluminescent signal that is generated can be detected usingroutine techniques known to those skilled in the art. Based on theintensity of the signal generated, the amount of GP73 or fucosylatedGP73 in the sample can be quantified. Specifically, the amount of GP73in the sample is proportional to the intensity of the signal generated.The amount of GP73 or fucosylated GP73 present can be quantified bycomparing the amount of light generated to a standard curve for GP73 orfucosylated GP73 or by comparison to a reference standard. The standardcurve can be generated using serial dilutions or solutions of knownconcentrations of GP73 or fucosylated GP73 by mass spectroscopy,gravimetric methods, and other techniques known in the art.

In a chemiluminescent microparticle assay employing the ARCHITECT® (orits successor) analyzer, the conjugate diluent pH should be about6.0+/−0.2, the microparticle coating buffer should be maintained at roomtemperature (i.e., at about 17 to about 27° C.), the microparticlecoating buffer pH should be about 6.5+/−0.2, and the microparticlediluent pH should be about 7.8+/−0.2. Solids preferably are less thanabout 0.2%, such as less than about 0.15%, less than about 0.14%, lessthan about 0.13%, less than about 0.12%, or less than about 0.11%, suchas about 0.10%.

(2) Methods of Using Anti-GP73 Antibodies

The present invention is directed to a method for determining thepresence, amount, or concentration of GP73 or GP73 fragment in a testsample using the disclosed anti-GP73 antibodies, or antibody fragmentsthereof. The method includes the steps of (a) contacting the test samplewith a capture antibody, which binds to an epitope on GP73 or GP73fragment, so as to form a capture antibody-GP73 or GP73 fragment antigencomplex; (b) contacting the capture antibody-GP73 or GP73 fragmentantigen complex with at least one detection antibody, which comprises adetectable label and binds to an epitope on GP73 or GP73 fragment thatis not bound by the capture antibody, to form a capture antibody-GP73 orGP73 fragment antigen-detection antibody complex; and (c) determiningthe presence, amount or concentration of GP73 or GP73 fragment in thetest sample based on the signal generated by the detectable label in thecapture antibody-GP73 or GP73 fragment antigen-detection antibodycomplex whereupon the present, amount, or concentration of GP73 or GP73fragment in the test sample is determined.

The capture antibody and detection antibody may be an anti-GP73 antibodydescribed above. For example, the capture antibody may include the1A-4246 antibody or a domain or region of: a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:1; a variable lightdomain comprising the amino acid sequence of SEQ ID NO:5; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:1 and avariable light domain comprising the amino acid sequence of SEQ ID NO:5;a variable heavy chain comprising a complementarity determining region(CDR)1 comprising the amino acid sequence of SEQ ID NO:2, a CDR2comprising the amino acid sequence of SEQ ID NO:3, and a CDR3 comprisingthe amino acid sequence of SEQ ID NO:4; a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:6, aCDR2 comprising the amino acid sequence of SEQ ID NO:7, and a CDR3comprising the amino acid sequence of SEQ ID NO:8; or a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4 and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8. The detectionantibody may include a 1B-4863 antibody or a domain or region of: avariable heavy domain comprising the amino acid sequence of SEQ ID NO:9;a variable light domain comprising the amino acid sequence of SEQ IDNO:13; a variable heavy domain comprising the amino acid sequence of SEQID NO:9 and a variable light domain comprising the amino acid sequenceof SEQ ID NO:13; a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:10, a CDR2 comprising the amino acidsequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:12; a variable light chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:14, a CDR2 comprising the amino acidsequence of SEQ ID NO:15, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:16; or a variable heavy chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12 and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16.

The present invention is also directed to a method for determining thepresence, amount, or concentration of fucosylated GP73 or fucosylatedGP73 fragment in a test sample using the disclosed anti-GP73 antibodies,or antibody fragments thereof. The method includes the steps of (a)contacting the test sample with a capture antibody, which binds to anepitope on fucosylated GP73 or fucosylated GP73 fragment, so as to forma capture antibody-fucosylated GP73 or fucosylated GP73 fragment antigencomplex; (b) contacting the capture antibody-fucosylated GP73 orfucosylated GP73 fragment antigen complex with at least one detectionantibody, which comprises a detectable label and binds to an epitope onfucosylated GP73 or fucosylated GP73 fragment that is not bound by thecapture antibody, to form a capture antibody-fucosylated GP73 orfucosylated GP73 fragment antigen-detection antibody complex; and (c)determining the presence, amount or concentration of fucosylated GP73 orfucosylated GP73 fragment in the test sample based on the signalgenerated by the detectable label in the capture antibody-fucosylatedGP73 or fucosylated GP73 fragment antigen-detection antibody complexwhereupon the present, amount, or concentration of fucosylated GP73 orfucosylated GP73 fragment in the test sample is determined, wherein thecapture binding protein comprises a protein, antibody or antibodyfragment whose binding to fucosylated GP73 or fucosylated GP73 fragmentis sensitive to the presence or absence of a fucose moiety on GP73 andthe detection binding protein comprises a protein, antibody or antibodyfragment whose binding to fucosylated GP73 or fucosylated GP73 fragmentis insensitive to the presence or absence of a fucose moiety on GP73.

The protein, antibody or antibody fragment whose binding to fucosylatedGP73 or fucosylated GP73 fragment is sensitive to the presence orabsence of a fucose sugar moiety on fucosylated GP73 or fucosylated GP73fragment may include Aleuria aurantia lectin (AAL) or a fragmentthereof; a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:25 or SEQ ID NO:33; a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:29 or SEQ ID NO:37; avariable heavy domain comprising the amino acid sequence of SEQ ID NO:25and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:29; a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:33 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:37; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28; a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:30, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:31, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:32; a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:38, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:39, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:40; a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2comprising the amino acid sequence of SEQ ID NO:31, and a CDR3comprising the amino acid sequence of SEQ ID NO:32; or a variable heavychain comprising a CDR 1 comprising the amino acid sequence of SEQ IDNO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, and aCDR3 comprising the amino acid sequence of SEQ ID NO:36, and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:38, a CDR2 comprising the amino acid sequence of SEQ ID NO:39, anda CDR3 comprising the amino acid sequence of SEQ ID NO:40.

The protein, antibody or antibody fragment whose binding to fucosylatedGP73 or fucosylated GP73 fragment is insensitive to the presence orabsence of a fucose sugar moiety on fucosylated GP73 or fucosylated GP73fragment may include a variable heavy domain region comprising the aminoacid sequence of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:17, or SEQ IDNO:41; a variable light domain region comprising the amino acid sequenceof SEQ ID NO:5, SEQ ID NO:13, SEQ ID NO:21, or SEQ ID NO:45; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:1 and avariable light domain region comprising the amino acid sequence of SEQID NO:5; a variable heavy domain comprising the amino acid sequence ofSEQ ID NO:9 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:13; a variable heavy domain comprising the aminoacid sequence of SEQ ID NO:17 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:21; a variable heavydomain comprising the amino acid sequence of SEQ ID NO:41 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:45;a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequence ofSEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4; a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:10, a CDR2 comprising the amino acid sequence ofSEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ IDNO:12; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequenceof SEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20; a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; a variable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequence ofSEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequenceof SEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:10, a CDR2 comprising the amino acid sequenceof SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ IDNO:12, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequenceof SEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequenceof SEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; or a variable heavy chain comprising a CDR 1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48.

The capture antibody and detection antibody may be an anti-GP73 antibodydescribed above. For example, the capture antibody may include the1A-4246 antibody or a domain or region of: a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:1; a variable lightdomain comprising the amino acid sequence of SEQ ID NO:5; a variableheavy domain comprising the amino acid sequence of SEQ ID NO:1 and avariable light domain comprising the amino acid sequence of SEQ ID NO:5;a variable heavy chain comprising a complementarity determining region(CDR)1 comprising the amino acid sequence of SEQ ID NO:2, a CDR2comprising the amino acid sequence of SEQ ID NO:3, and a CDR3 comprisingthe amino acid sequence of SEQ ID NO:4; a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:6, aCDR2 comprising the amino acid sequence of SEQ ID NO:7, and a CDR3comprising the amino acid sequence of SEQ ID NO:8; or a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4 and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8. The detectionantibody may include a 1B-4863 antibody or a domain or region of: avariable heavy domain comprising the amino acid sequence of SEQ ID NO:9;a variable light domain comprising the amino acid sequence of SEQ IDNO:13; a variable heavy domain comprising the amino acid sequence of SEQID NO:9 and a variable light domain comprising the amino acid sequenceof SEQ ID NO:13; a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:10, a CDR2 comprising the amino acidsequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:12; a variable light chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:14, a CDR2 comprising the amino acidsequence of SEQ ID NO:15, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:16; or a variable heavy chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12 and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16.

b. Controls

It may be desirable to include a control sample. The control sample maybe analyzed concurrently with the sample from the subject as describedabove. The results obtained from the subject sample can be compared tothe results obtained from the control sample. Standard curves may beprovided, with which assay results for the biological sample may becompared. Such standard curves present levels of marker as a function ofassay units, i.e. fluorescent signal intensity, if a fluorescent labelis used. Using samples taken from multiple donors, standard curves canbe provided for control levels of the GP73 or fucosylated GP73 in normalhealthy tissue, as well as for “at-risk” levels of the GP73 orfucosylated GP73 in tissue taken from donors, who may have one or moreof the characteristics set forth above.

Thus, in view of the above, a method for determining the presence,amount, or concentration of GP73 or fucosylated GP73 in a test sample isprovided. The method comprises assaying the test sample for GP73 orfucosylated GP73 by an immunoassay, for example, employing at least onecapture antibody that binds to an epitope on GP73 or fucosylated GP73 oror fucosylated GP73 and at least one detection antibody that binds to anepitope on GP73 or fucosylated GP73 which is different from the epitopefor the capture antibody and optionally includes a detectable label, andcomprising comparing a signal generated by the detectable label as adirect or indirect indication of the presence, amount or concentrationof GP73 or fucosylated GP73 in the test sample to a signal generated asa direct or indirect indication of the presence, amount or concentrationof GP73 or fucosylated GP73 in a calibrator. The calibrator isoptionally, and is preferably, part of a series of calibrators in whicheach of the calibrators differs from the other calibrators in the seriesby the concentration of GP73 or fucosylated GP73.

5. METHODS OF DIAGNOSING, PROGNOSTICATING, OR ASSESSING THE EFFICACY OFA THERAPEUTIC/PROPHYLACTIC TREATMENT

The method can further comprise diagnosing, prognosticating, orassessing the efficacy of a therapeutic/prophylactic treatment of apatient from whom the test sample was obtained. If the method furthercomprises assessing the efficacy of a therapeutic/prophylactic treatmentof the patient from whom the test sample was obtained, the methodoptionally further comprises modifying the therapeutic/prophylactictreatment of the patient as needed to improve efficacy. By measuring anddetecting GP73 and fucosylated GP73, the method allows for more diseasesto be more accurately diagnosed and subsequently treated moresuccessfully, compared to other commercially available GP73immunoassays. The method can be adapted for use in an automated systemor a semi-automated system.

Generally, a predetermined level can be employed as a benchmark againstwhich to assess results obtained upon assaying a test sample for GP73 orfucosylated GP73. Generally, in making such a comparison, thepredetermined level is obtained by running a particular assay asufficient number of times and under appropriate conditions such that alinkage or association of analyte presence, amount or concentration witha particular stage or endpoint of a disease, disorder or condition(e.g., liver disease or cancer) or with particular indicia can be made.Typically, the predetermined level is obtained with assays of referencesubjects (or populations of subjects). The GP73 measured can includeGP73 fragments thereof, degradation products thereof, and/or enzymaticcleavage products thereof. The fucosylated GP73 can include glycosylatedGP73 fragments thereof, degradation products thereof, and/or enzymaticcleavage products thereof.

In particular, with respect to a predetermined level as employed formonitoring disease progression and/or treatment, the amount orconcentration of GP73 or GP73 fragment or fucosylated GP73 or orfucosylated GP73 fragment may be “unchanged,” “favorable” (or “favorablyaltered”), or “unfavorable” (or “unfavorably altered”). “Elevated” or“increased” refers to an amount or a concentration in a test sample thatis higher or greater than a typical or normal level or range (e.g.,predetermined level), or is higher or greater than another referencelevel or range (e.g., earlier or baseline sample). The term “lowered” or“reduced” refers to an amount or a concentration in a test sample thatis lower or less than a typical or normal level or range (e.g.,predetermined level), or is lower or less than another reference levelor range (e.g., earlier or baseline sample). The term “altered” refersto an amount or a concentration in a sample that is altered (increasedor decreased) over a typical or normal level or range (e.g.,predetermined level), or over another reference level or range (e.g.,earlier or baseline sample).

The typical or normal level or range for GP73 or fucosylated GP73 isdefined in accordance with standard practice. A so-called altered levelor alteration can be considered to have occurred when there is any netchange as compared to the typical or normal level or range, or referencelevel or range that cannot be explained by experimental error or samplevariation. Thus, the level measured in a particular sample will becompared with the level or range of levels determined in similar samplesfrom a so-called normal subject. In this context, a “normal subject” isan individual with no detectable disease or disorder, and a “normal”(sometimes termed “control”) patient or population is/are one(s) thatexhibit(s) no detectable disease or disorder, respectively, for example.An “apparently normal subject” is one in which GP73 or fucosylated GP73or fucosylated GP73 has not been or is being assessed. The level of ananalyte is said to be “elevated” when the analyte is normallyundetectable (e.g., the normal level is zero, or within a range of fromabout 25 to about 75 percentiles of normal populations), but is detectedin a test sample, as well as when the analyte is present in the testsample at a higher than normal level. Thus, inter alia, the disclosureprovides a method of screening for a subject having, or at risk ofhaving, liver disease or cancer.

a. Methods of Providing a Diagnosis of a Subject Having Disease

The method described herein can be used to provide a diagnosis of asubject having disease by determining the levels of GP73 or fucosylatedGP73 in a subject. The method may be used to detect disease in a subjectusing the anti-GP73 antibodies described above, or antibody fragmentsthereof. The method includes the steps of (a) obtaining a biologicalsample from a subject, (b) determining the level of GP73 or fucosylatedGP73 in the biological sample using anti-GP73 antibodies, or antibodyfragments thereof, (c) comparing the level of GP73 or fucosylated GP73in the biological sample to a reference level of GP73 or fucosylatedGP73, (d) identifying the subject as having disease if the level of GP73or fucosylated GP73 in the biological sample is greater than thereference level of GP73 or fucosylated GP73, and (e) administering atreatment regimen to the subject identified as having disease. Theanti-GP73 antibodies used in the method may be the 1A-4246 antibody and1B-4863 antibody, or antibody fragments thereof

(1) Liver Disease

The method described herein can be used to provide a diagnosis of asubject having liver disease by determining the levels of GP73 orfucosylated GP73 in a subject. The method may be used to detect liverdisease in a subject using the anti-GP73 antibodies described above, orantibody fragments thereof. The method includes the steps of (a)obtaining a biological sample from a subject, (b) determining the levelof GP73 or fucosylated GP73 in the biological sample using anti-GP73antibodies, or antibody fragments thereof, (c) comparing the level ofGP73 or fucosylated GP73 in the biological sample to a reference levelof GP73 or fucosylated GP73, (d) identifying the subject as having liverdisease if the level of GP73 or fucosylated GP73 in the biologicalsample is greater than the reference level of GP73 or fucosylated GP73,and (e) administering a treatment regimen to the subject identified ashaving liver disease. The anti-GP73 antibodies used in the method may bethe 1A-4246 antibody and 1B-4863 antibody, or antibody fragmentsthereof.

The reference level in this method can be the level of GP73 orfucosylated GP73 in a patient having liver disease. Levels higher thanor equal to 0.30 ng/mL, 0.31 ng/mL, 0.32 ng/mL, 0.33 ng/mL, 0.34 ng/mL,0.35 ng/mL, 0.36 ng/mL, 0.37 ng/mL, 0.38 ng/mL, 0.39 ng/mL, 0.40 ng/mL,0.41 ng/mL, 0.42 ng/mL, 0.43 ng/mL, 0.44 ng/mL, 0.45 ng/mL, 0.46 ng/mL,0.47 ng/mL, 0.48 ng/mL, 0.49 ng/mL, 0.50 ng/mL, 0.51 ng/mL, 0.52 ng/mL,0.53 ng/mL, 0.54 ng/mL, 0.55 ng/mL, 0.56 ng/mL, 0.57 ng/mL, 0.58 ng/mL,0.59 ng/mL, 0.60 ng/mL, 0.61 ng/mL, 0.62 ng/mL, 0.63 ng/mL, 0.64 ng/mL,0.65 ng/mL, 0.66 ng/mL, 0.67 ng/mL, 0.68 ng/mL, 0.69 ng/mL, 0.70 ng/mL,0.71 ng/mL, 0.72 ng/mL, 0.73 ng/mL, 0.74 ng/mL, 0.75 ng/mL, 0.76 ng/mL,0.77 ng/mL, 0.78 ng/mL, 0.79 ng/mL, 0.80 ng/mL, 0.81 ng/mL, 0.82 ng/mL,0.83 ng/mL, 0.84 ng/mL, 0.85 ng/mL, 0.86 ng/mL, 0.87 ng/mL, 0.88 ng/mL,0.89 ng/mL, 0.90 ng/mL, 0.91 ng/mL, or 0.92 ng/mL in serum of GP73 orfucosylated GP73 identify the subject as having liver disease.

(a) Liver Cirrhosis

The method described herein can be used to provide a diagnosis of asubject having liver cirrhosis by determining the levels of GP73 orfucosylated GP73 in a subject. The method may be used to detect livercirrhosis in a subject using the anti-GP73 antibodies described above,or antibody fragments thereof. The method includes the steps of (a)obtaining a biological sample from a subject, (b) determining the levelof GP73 or fucosylated GP73 in the biological sample using anti-GP73antibodies, or antibody fragments thereof, (c) comparing the level ofGP73 or fucosylated GP73 in the biological sample to a reference levelof GP73 or fucosylated GP73, (d) identifying the subject as having livercirrhosis if the level of GP73 or fucosylated GP73 in the biologicalsample is greater than the reference level of GP73 or fucosylated GP73,and (e) administering a treatment regimen to the subject identified ashaving liver cirrhosis. The anti-GP73 antibodies used in the method maybe the 1A-4246 antibody and 1B-4863 antibody, or antibody fragmentsthereof.

The reference level in this method can be the level of GP73 orfucosylated GP73 in a patient having liver cirrhosis. Levels higher thanor equal to 0.68 ng/mL, 0.69 ng/mL, 0.70 ng/mL, 0.71 ng/mL, 0.72 ng/mL,0.73 ng/mL, 0.74 ng/mL, 0.75 ng/mL, 0.76 ng/mL, 0.77 ng/mL, 0.78 ng/mL,0.79 ng/mL, 0.80 ng/mL, 0.81 ng/mL, 0.82 ng/mL, 0.83 ng/mL, 0.84 ng/mL,0.85 ng/mL, 0.86 ng/mL, 0.87 ng/mL, 0.88 ng/mL, 0.89 ng/mL, 0.90 ng/mL,0.91 ng/mL, or 0.92 ng/mL in serum of GP73 or fucosylated GP73 identifythe subject as having liver cirrhosis.

(b) Liver Cancer

The method described herein can be used to provide a diagnosis of asubject having liver cancer by determining the levels of GP73 orfucosylated GP73 in a subject. The method may be used to detect livercancer in a subject using the anti-GP73 antibodies described above, orantibody fragments thereof. The method includes the steps of (a)obtaining a biological sample from a subject, (b) determining the levelof GP73 or fucosylated GP73 in the biological sample using the anti-GP73antibodies described above, or antibody fragments thereof, (c) comparingthe level of GP73 or fucosylated GP73 in the biological sample to areference level of GP73 or fucosylated GP73, (d) identifying the subjectas having liver cancer if the level of GP73 or fucosylated GP73 in thebiological sample is greater than the reference level of GP73 orfucosylated GP73, and (e) administering a treatment regimen to thesubject identified as having liver cancer. The anti-GP73 antibodies usedin the method may be the 1A-4246 antibody and 1B-4863 antibody, orantibody fragments thereof.

The reference level in this method can be the level of GP73 orfucosylated GP73 in a patient having liver cancer. Levels higher than orequal to 0.30 ng/mL, 0.31 ng/mL, 0.32 ng/mL, 0.33 ng/mL, 0.34 ng/mL,0.35 ng/mL, 0.36 ng/mL, 0.37 ng/mL, 0.38 ng/mL, 0.39 ng/mL, 0.40 ng/mL,0.45 ng/mL, 0.50 ng/mL, 0.55 ng/mL, 0.58 ng/mL, 0.59 ng/mL, 0.60 ng/mL,0.61 ng/mL, 0.62 ng/mL, 0.63 ng/mL, 0.64 ng/mL, 0.65 ng/mL, 0.66 ng/mL,0.67 ng/mL, or 0.68 ng/mL in serum of GP73 or fucosylated GP73 identifythe subject as having liver cancer.

(2) Cancer

The method described herein can be used to provide a diagnosis of asubject having cancer by determining the levels of GP73 or fucosylatedGP73 in a subject. The method may be used to detect cancer in a subjectusing the anti-GP73 antibodies described above, or antibody fragmentsthereof. The method includes the steps of (a) obtaining a biologicalsample from a subject, (b) determining the level of GP73 or fucosylatedGP73 in the biological sample using anti-GP73 antibodies, or antibodyfragments thereof, (c) comparing the level of GP73 or fucosylated GP73in the biological sample to a reference level of GP73 or fucosylatedGP73, (d) identifying the subject as having cancer if the level of GP73or fucosylated GP73 in the biological sample is greater than thereference level of GP73 or fucosylated GP73, and (e) administering atreatment regimen to the subject identified as having cancer. Theanti-GP73 antibodies used in the method may be the 1A-4246 antibody and1B-4863 antibody, or antibody fragments thereof.

The reference level in this method can be the level of GP73 orfucosylated GP73 in a patient having cancer. Levels higher than or equalto 0.29 ng/mL, 0.30 ng/mL, 0.31 ng/mL, 0.32 ng/mL, 0.33 ng/mL, 0.34ng/mL, 0.35 ng/mL, 0.36 ng/mL, 0.37 ng/mL, 0.38 ng/mL, 0.39 ng/mL, 0.40ng/mL, 0.41 ng/mL, 0.42 ng/mL, 0.43 ng/mL, 0.44 ng/mL, 0.45 ng/mL, 0.46ng/mL, 0.47 ng/mL, 0.48 ng/mL, 0.49 ng/mL, 0.50 ng/mL, 0.51 ng/mL, 0.52ng/mL, 0.53 ng/mL, 0.54 ng/mL, 0.55 ng/mL, 0.56 ng/mL, 0.57 ng/mL, 0.58ng/mL, 0.59 ng/mL, 0.60 ng/mL, 0.61 ng/mL, 0.62 ng/mL, 0.63 ng/mL, 0.64ng/mL, 0.65 ng/mL, 0.66 ng/mL, 0.67 ng/mL, 0.68 ng/mL, 0.69 ng/mL, 0.70ng/mL, 0.71 ng/mL, 0.72 ng/mL, 0.73 ng/mL, 0.74 ng/mL, 0.75 ng/mL, 0.76ng/mL, 0.77 ng/mL, 0.78 ng/mL, 0.79 ng/mL, 0.80 ng/mL, 0.81 ng/mL, 0.82ng/mL, 0.83 ng/mL, 0.84 ng/mL, 0.85 ng/mL, 0.86 ng/mL, 0.87 ng/mL, 0.88ng/mL, 0.89 ng/mL, 0.90 ng/mL, 0.91 ng/mL, 0.92 ng/mL, 0.93 ng/mL, 0.94ng/mL, 0.95 ng/mL, 0.96 ng/mL, 0.97 ng/mL, 0.98 ng/mL, 0.99 ng/mL, 1.00ng/mL, 1.01 ng/mL, 1.02 ng/mL, 1.03 ng/mL, 1.04 ng/mL, 1.05 ng/mL, 1.06ng/mL, 1.07 ng/mL, 1.08 ng/mL, 1.09 ng/mL, or 1.10 ng/mL in serum ofGP73 or fucosylated GP73 identify the subject as having cancer.

b. Methods for Determining the Risk of a Subject of Developing LiverDisease

The methods described herein also can be used to determine whether ornot a subject has or is at risk of developing liver disease bydetermining the levels of GP73 or fucosylated GP73 in a subject usingthe anti-GP73 antibodies described above, or antibody fragments thereof.Thus, in particular embodiments, the disclosure also provides a methodfor determining whether a subject having, or at risk for, liverdiseases, discussed herein and known in the art, is a candidate fortherapy or treatment. Generally, the subject is one who has experiencedsome symptom of the disease or who has actually been diagnosed ashaving, or being at risk for, such a disease, and/or who demonstrates anunfavorable concentration or amount of GP73 or GP73 fragment, asdescribed herein.

Specifically, such a method can comprise the steps of: (a) determiningthe concentration or amount in a test sample from a subject of GP73 orfucosylated GP73 using the methods described herein, or methods known inthe art); and (b) comparing the concentration or amount of GP73 orfucosylated GP73 determined in step (a) with a predetermined level,wherein, if the concentration or amount of GP73 or fucosylated GP73determined in step (a) is favorable with respect to a predeterminedlevel, then the subject is determined not to have or be at risk forliver disease as discussed herein and known in the art. However, if theconcentration or amount of GP73 or fucosylated GP73 determined in step(a) is unfavorable with respect to the predetermined level, then thesubject is determined to have or be at risk for liver disease asdiscussed herein and known in the art. The liver disease may be livercirrhosis or liver cancer.

c. Methods for Determining the Risk of a Subject Developing Cancer

The methods described herein also can be used to determine whether ornot a subject has or is at risk of developing cancer, by determining thelevels of GP73 or fucosylated GP73 in a subject using the anti-GP73antibodies described above, or antibody fragments thereof. Thus, inparticular embodiments, the disclosure also provides a method fordetermining whether a subject having, or at risk for, cancer, asdiscussed herein and known in the art, is a candidate for therapy ortreatment. Generally, the subject is one who has experienced somesymptom of the disease or who has actually been diagnosed as having, orbeing at risk for, such a disease, and/or who demonstrates anunfavorable concentration or amount of GP73 or fucosylated GP73, asdescribed herein.

Specifically, such a method can comprise the steps of: (a) determiningthe concentration or amount in a test sample from a subject of GP73 orfucosylated GP73 using the methods described herein, or methods known inthe art), and (b) comparing the concentration or amount of GP73 orfucosylated GP73 determined in step (a) with a predetermined level,wherein, if the concentration or amount of GP73 or fucosylated GP73determined in step (a) is favorable with respect to a predeterminedlevel, then the subject is determined not to have or be at risk forcancer as discussed herein and known in the art. However, if theconcentration or amount of GP73 determined in step (a) is unfavorablewith respect to the predetermined level, then the subject is determinedto have or be at risk for cancer as discussed herein and known in theart. The cancer may be colon cancer, gastric cancer, breast cancer,prostate cancer or liver cancer.

d. Methods of Monitoring the Progression of Disease in a Subject

The methods described herein also can be used to monitor the progressionof disease, such as liver disease or cancer, in a subject by determiningthe levels of GP73 or fucosylated GP73 in a subject using the anti-GP73antibodies described above, or antibody fragments thereof. Optimally,the method includes the steps of (a) determining the concentration oramount of GP73 or fucosylated GP73 in a test sample from a subject usingthe anti-GP73 antibodies described above, or antibody fragments thereof,(b) determining the concentration or amount of GP73 or fucosylated GP73in a later test sample from a subject using the anti-GP73 antibodiesdescribed above, or antibody fragments thereof, and (c) comparing theconcentration or amount of GP73 or fucosylated GP73 as determined instep (b) with the concentration or amount of GP73 or fucosylated GP73determined in step (a), wherein if the concentration or amountdetermined in step (b) is unchanged or is unfavorable when compared tothe concentration or amount of GP73 or fucosylated GP73 determined instep (a), then the disease in the subject is determined to havecontinued, progressed or worsened. By comparison, if the concentrationor amount of GP73 or fucosylated GP73 as determined in step (b) isfavorable when compared to the concentration or amount of GP73 orfucosylated GP73 as determined in step (a), then the disease in thesubject is determined to have discontinued, regressed or improved.

Optionally, the method further comprises comparing the concentration oramount of GP73 or fucosylated GP73 as determined in step (b), forexample, with a predetermined level. Further, optionally the methodcomprises treating the subject with one or more pharmaceuticalcompositions for a period of time if the comparison shows that theconcentration or amount of GP73 or fucosylated GP73 as determined instep (b), for example, is unfavorably altered with respect to thepredetermined level.

Still further, the methods can be used to monitor treatment in a subjectreceiving treatment with one or more pharmaceutical compositions.Specifically, such methods involve providing a first test sample from asubject before the subject has been administered one or morepharmaceutical compositions. Next, the concentration or amount in afirst test sample from a subject of GP73 or fucosylated GP73 isdetermined (e.g., using the methods described herein or as known in theart). After the concentration or amount of GP73 or fucosylated GP73 isdetermined, optionally the concentration or amount of GP73 orfucosylated GP73 is then compared with a predetermined level. If theconcentration or amount of GP73 or fucosylated GP73 as determined in thefirst test sample is lower than the predetermined level, then thesubject is not treated with one or more pharmaceutical compositions oralternatively, the subject may be treated with one or morepharmaceutical compositions. If the concentration or amount of GP73 orfucosylated GP73 as determined in the first test sample is higher thanthe predetermined level, then the subject is treated with one or morepharmaceutical compositions for a period of time or alternatively, thesubject is not treated with one or more pharmaceutical compositions. Theperiod of time that the subject is treated with the one or morepharmaceutical compositions can be determined by one skilled in the art(for example, the period of time can be from about seven (7) days toabout two years, preferably from about fourteen (14) days to about one(1) year).

During the course of treatment with the one or more pharmaceuticalcompositions, second and subsequent test samples are then obtained fromthe subject. The number of test samples and the time in which said testsamples are obtained from the subject are not critical. For example, asecond test sample could be obtained seven (7) days after the subject isfirst administered the one or more pharmaceutical compositions, a thirdtest sample could be obtained two (2) weeks after the subject is firstadministered the one or more pharmaceutical compositions, a fourth testsample could be obtained three (3) weeks after the subject is firstadministered the one or more pharmaceutical compositions, a fifth testsample could be obtained four (4) weeks after the subject is firstadministered the one or more pharmaceutical compositions, etc.

After each second or subsequent test sample is obtained from thesubject, the concentration or amount of GP73 or or fucosylated GP73 isdetermined in the second or subsequent test sample is determined (e.g.,using the methods described herein or as known in the art). Theconcentration or amount of GP73 or fucosylated GP73 as determined ineach of the second and subsequent test samples is then compared with theconcentration or amount of GP73 or fucosylated GP73 as determined in thefirst test sample (e.g., the test sample that was originally optionallycompared to the predetermined level). If the concentration or amount ofGP73 or fucosylated GP73 as determined in step (c) is favorable whencompared to the concentration or amount of GP73 or fucosylated GP73 asdetermined in step (a), then the disease in the subject is determined tohave discontinued, regressed, or improved, and the subject shouldcontinue to be administered the one or pharmaceutical compositions ofstep (b). However, if the concentration or amount determined in step (c)is unchanged or is unfavorable when compared to the concentration oramount of GP73 or fucosylated GP73 as determined in step (a), then thedisease in the subject is determined to have continued, progressed orworsened, and the subject should be treated with a higher concentrationof the one or more pharmaceutical compositions administered to thesubject in step (b) or the subject should be treated with one or morepharmaceutical compositions that are different from the one or morepharmaceutical compositions administered to the subject in step (b).Specifically, the subject can be treated with one or more pharmaceuticalcompositions that are different from the one or more pharmaceuticalcompositions that the subject had previously received to decrease orlower said subject's GP73 or fucosylated GP73 level.

Generally, for assays in which repeat testing may be done (e.g.,monitoring disease progression and/or response to treatment), a secondor subsequent test sample is obtained at a period in time after thefirst test sample has been obtained from the subject. Specifically, asecond test sample from the subject can be obtained minutes, hours,days, weeks or years after the first test sample has been obtained fromthe subject. For example, the second test sample can be obtained fromthe subject at a time period of about 1 minute, about 5 minutes, about10 minutes, about 15 minutes, about 30 minutes, about 45 minutes, about60 minutes, about 2 hours, about 3 hours, about 4 hours, about 5 hours,about 6 hours, about 7 hours, about 8 hours, about 9 hours, about 10hours, about 11 hours, about 12 hours, about 13 hours, about 14 hours,about 15 hours, about 16 hours, about 17 hours, about 18 hours, about 19hours, about 20 hours, about 21 hours, about 22 hours, about 23 hours,about 24 hours, about 2 days, about 3 days, about 4 days, about 5 days,about 6 days, about 7 days, about 2 weeks, about 3 weeks, about 4 weeks,about 5 weeks, about 6 weeks, about 7 weeks, about 8 weeks, about 9weeks, about 10 weeks, about 11 weeks, about 12 weeks, about 13 weeks,about 14 weeks, about 15 weeks, about 16 weeks, about 17 weeks, about 18weeks, about 19 weeks, about 20 weeks, about 21 weeks, about 22 weeks,about 23 weeks, about 24 weeks, about 25 weeks, about 26 weeks, about 27weeks, about 28 weeks, about 29 weeks, about 30 weeks, about 31 weeks,about 32 weeks, about 33 weeks, about 34 weeks, about 35 weeks, about 36weeks, about 37 weeks, about 38 weeks, about 39 weeks, about 40 weeks,about 41 weeks, about 42 weeks, about 43 weeks, about 44 weeks, about 45weeks, about 46 weeks, about 47 weeks, about 48 weeks, about 49 weeks,about 50 weeks, about 51 weeks, about 52 weeks, about 1.5 years, about 2years, about 2.5 years, about 3.0 years, about 3.5 years, about 4.0years, about 4.5 years, about 5.0 years, about 5.5. years, about 6.0years, about 6.5 years, about 7.0 years, about 7.5 years, about 8.0years, about 8.5 years, about 9.0 years, about 9.5 years or about 10.0years after the first test sample from the subject is obtained. Whenused to monitor disease progression, the above assay can be used tomonitor the progression of disease in subjects suffering from acuteconditions. Acute conditions, also known as critical care conditions,refer to acute, life-threatening diseases or other critical medicalconditions involving, for example, the cardiovascular system orexcretory system. Typically, critical care conditions refer to thoseconditions requiring acute medical intervention in a hospital-basedsetting (including, but not limited to, the emergency room, intensivecare unit, trauma center, or other emergent care setting) oradministration by a paramedic or other field-based medical personnel.For critical care conditions, repeat monitoring is generally done withina shorter time frame, namely, minutes, hours or days (e.g., about 1minute, about 5 minutes, about 10 minutes, about 15 minutes, about 30minutes, about 45 minutes, about 60 minutes, about 2 hours, about 3hours, about 4 hours, about 5 hours, about 6 hours, about 7 hours, about8 hours, about 9 hours, about 10 hours, about 11 hours, about 12 hours,about 13 hours, about 14 hours, about 15 hours, about 16 hours, about 17hours, about 18 hours, about 19 hours, about 20 hours, about 21 hours,about 22 hours, about 23 hours, about 24 hours, about 2 days, about 3days, about 4 days, about 5 days, about 6 days or about 7 days), and theinitial assay likewise is generally done within a shorter timeframe,e.g., about minutes, hours or days of the onset of the disease orcondition.

The assays also can be used to monitor the progression of disease insubjects suffering from chronic or non-acute conditions. Non-criticalcare conditions or non-acute conditions, refers to conditions other thanacute, life-threatening disease or other critical medical conditionsinvolving, for example, the cardiovascular system and/or excretorysystem. Typically, non-acute conditions include those of longer-term orchronic duration. For non-acute conditions, repeat monitoring generallyis done with a longer timeframe, e.g., hours, days, weeks, months oryears (e.g., about 1 hour, about 2 hours, about 3 hours, about 4 hours,about 5 hours, about 6 hours, about 7 hours, about 8 hours, about 9hours, about 10 hours, about 11 hours, about 12 hours, about 13 hours,about 14 hours, about 15 hours, about 16 hours, about 17 hours, about 18hours, about 19 hours, about 20 hours, about 21 hours, about 22 hours,about 23 hours, about 24 hours, about 2 days, about 3 days, about 4days, about 5 days, about 6 days, about 7 days, about 2 weeks, about 3weeks, about 4 weeks, about 5 weeks, about 6 weeks, about 7 weeks, about8 weeks, about 9 weeks, about 10 weeks, about 11 weeks, about 12 weeks,about 13 weeks, about 14 weeks, about 15 weeks, about 16 weeks, about 17weeks, about 18 weeks, about 19 weeks, about 20 weeks, about 21 weeks,about 22 weeks, about 23 weeks, about 24 weeks, about 25 weeks, about 26weeks, about 27 weeks, about 28 weeks, about 29 weeks, about 30 weeks,about 31 weeks, about 32 weeks, about 33 weeks, about 34 weeks, about 35weeks, about 36 weeks, about 37 weeks, about 38 weeks, about 39 weeks,about 40 weeks, about 41 weeks, about 42 weeks, about 43 weeks, about 44weeks, about 45 weeks, about 46 weeks, about 47 weeks, about 48 weeks,about 49 weeks, about 50 weeks, about 51 weeks, about 52 weeks, about1.5 years, about 2 years, about 2.5 years, about 3.0 years, about 3.5years, about 4.0 years, about 4.5 years, about 5.0 years, about 5.5.years, about 6.0 years, about 6.5 years, about 7.0 years, about 7.5years, about 8.0 years, about 8.5 years, about 9.0 years, about 9.5years or about 10.0 years), and the initial assay likewise generally isdone within a longer time frame, e.g., about hours, days, months oryears of the onset of the disease or condition.

Furthermore, the above assays can be performed using a first test sampleobtained from a subject where the first test sample is obtained from onesource, such as urine, serum, or plasma. Optionally the above assays canthen be repeated using a second test sample obtained from the subjectwhere the second test sample is obtained from another source. Forexample, if the first test sample was obtained from urine, the secondtest sample can be obtained from serum or plasma. The results obtainedfrom the assays using the first test sample and the second test samplecan be compared. The comparison can be used to assess the status of adisease or condition in the subject.

e. Methods for Determining if a Subject is Predisposed to or Sufferingfrom a Disease

Moreover, the methods described herein also can be used to determinewhether a subject predisposed to or suffering from a disease (e.g.,liver disease or cancer, as discussed herein and known in the art) willbenefit from treatment. In particular, the disclosure relates to GP73companion diagnostic methods and products. Thus, the method of“monitoring the treatment of disease in a subject” as described hereinfurther optimally also can encompass selecting or identifying candidatesfor liver disease treatments, such as liver resection and livertransplant, or for cancer treatments, such as surgery, radiationtherapy, targeted therapy, and chemotherapy.

f. Methods for Determining if a Subject is Responding to theAdministration of a Pharmaceutical Composition

The methods described herein also can be used to determine if a subjectis responding to the administration of one or more pharmaceuticalcompositions by determining the levels of GP73 or fucosylated GP73 inthe subject using the anti-GP73 antibodies described above, or antibodyfragments thereof. The method optionally comprises an assay as describedherein, where the level of GP73 or fucosylated GP73 is assessed beforeand following treatment of the subject with one or more pharmaceuticalcompositions (e.g., particularly with a pharmaceutical related to amechanism of action involving GP73 or fucosylated GP73), or where thelevel of GP73 is assessed following such treatment and the concentrationor the level of GP73 or fucosylated GP73 is compared against apredetermined level. An unfavorable concentration of amount of GP73 orfucosylated GP73 observed following treatment confirms that the subjectwill not benefit from receiving further or continued treatment, whereasa favorable concentration or amount of GP73 or fucosylated GP73 observedfollowing treatment confirms that the subject will benefit fromreceiving further or continued treatment. This confirmation assists withmanagement of clinical studies, and provision of improved patient care.

The method includes the steps of (a) obtaining a biological sample froma subject, (b) determining the level of GP73 or fucosylated GP73 in thebiological sample using the anti-GP73 antibodies described above, orantibody fragments thereof, (c) comparing the level of GP73 orfucosylated GP73 in the biological sample to a reference level of GP73or fucosylated GP73, wherein an altered concentration of GP73 orfucosylated GP73 indicates that the subject is not responding to theadministration of one or more pharmaceutical compositions, and (d)adjusting the treatment of the subject if the subject is not respondingto the administration of one or more pharmaceutical compositions.

6. COMBINATION OF BIOMARKERS

The antibodies and methods described above may be used to detect andmeasure levels and concentrations of GP73 or fucosylated GP73 incombination with one or more biomarkers or immunoassays specific fordisease. The combination of GP73 or fucosylated GP73 with one or morebiomarkers or immunoassays specific for disease may provide a greaterdiscrimination between healthy controls and individuals with diseasecompared to measuring GP73 or fucosylated GP73 alone. For example,measure a panel of GP73 or fucosylated GP73 and additional liver diseasebiomarkers may provide a greater discrimination between healthy controlsand individuals with disease compared to a panel of GP73 or fucosylatedGP73 alone. The combination of GP73 or fucosylated GP73 with at leastone or more biomarkers may provide greater discrimination betweenhealthy controls and individuals who have liver disease and/orindividuals who have cancer.

Examples of the one or more biomarker include protein induced by theabsence of vitamin K or antagonist-II (PIVKA-II), α-fetoprotein (AFP),AFP-L3, hemopexin (HPX), fucosylated hemopexin (Fuc-HPX), fucosylatedkininogen (Fc-Kin), and fucosylated a-1-antitrypsin (F-AT).

a. PIVKA-II

Protein induced by the absence of vitamin K or antagonist-II (PIVKA-II),also known as des-g-carboxy prothrombin (DCP), is an abnormal form ofthe coagulation protein, prothrombin and is widely used with AFP inJapan during and after HCC treatment to predict adverse outcomes, and todetect early recurrence and potential malignancy. PIVKA-II is the leastsensitive to risk factors for HCC (such as cirrhosis), and hence themost useful in predicting HCC. It differentiates HCC from non-malignantliver diseases, however, false increases of PIVKA-II concentrations arefound in patients with severe obstructive jaundice due to intrahepaticcholestasis, or under conditions in which the action of vitamin K isimpaired in individuals with long standing vitamin K deficiency, and inthose who have ingested Warfarin or wide spectrum antibiotics.

b. AFP

Alpha-Fetal Protein (AFP), also known as α-fetoprotein,alpha-1-fetoprotein and alpha-fetoglobulin, is a protein that in humansis encoded by the AFP gene. AFP is widely used in clinical practiceworldwide for screening patients at high-risk for HCC, for the diagnosisand monitoring of HCC patients in conjunction with ultrasound to detectearly recurrence, for monitoring the response to therapy, and fordetecting early relapse. However, AFP can be produced under manycircumstances, including other liver diseases, leading to falsepositives and is not present in all patients with HCC.

The Lens culinaris hemagglutinin reactive fraction of AFP (AFP-L3) is afucosylated glycoform of AFP, which may be used as a marker to representthe degree of biological malignancy of HCC. An AFP-L3 assay has alsobeen approved by the US Food and Drug Administration for diagnosis ofHCC because of its higher specificity than AFP.

c. HPX

Hemopexin (HPX), also known as beta-1B-glycoprotein, is encoded by theHPX gene. HPX is expressed primarily in the liver and is an abundant andacute phase protein in human serum. HPX binds heme with the highestaffinity of any known protein. Its function is to scavenge the hemereleased or lost by the turnover of heme proteins such as hemoglobin andthus protects the body from the oxidative damage that free heme cancause. In addition, HPX releases its bound ligand for internalisationupon interacting with a specific receptor situated on the surface ofliver cells. This function of HPX is to preserve the body's iron. Thisprotein has five glucosamine oligosaccharides that are N-linked to theacceptor sequence, Asn-X-Ser/Thr. Fucosylated HPX (Fuc-HPX) has two corefucosylation sites of HPX in human serum, which were identified using aproteomics approach. Fucosylated hemopexin (Fuc-HPX) may be a potentialbiomarker for HCC as levels of Fuc-HPX in the Lens culinarishemagglutinin lectin bound serum fraction showed good diagnosticaccuracy for HCC in a study using a small sample set.

d. Serum Glycoproteins

Changes in glycosylation have been associated with the development ofcirrhosis and HCC has been reported. The altered glycosylation of serumglycoproteins, such as fucosylated kininogen (Fc-kin) and fucosylateda1-antitrypsin (F-AT), may be potential biomarkers when usedindependently or in combination with other HCC markers.

7. TREATMENT OF SUBJECTS SUFFERING FROM LIVER DISEASE

The subject identified in the methods described above having levels ofGP73 greater than or equal to the values discussed above is identifiedas a patient suffering from liver disease. The subject is then treatedfor the liver disease. Treatment of liver disease may include smallmolecule GP73 or fucosylated GP73 inhibitors and anti-GP73 neutralizingantibodies.

a. Liver Cirrhosis

The subject identified in the methods described above having levels ofGP73 or fucosylated GP73 greater than or equal to the values discussedabove is identified as a patient suffering from liver cirrhosis. Thesubject is then treated for liver cirrhosis. Treatment may include alow-sodium diet, medications, surgery, such as liver transplant,paracentesis with or without a protein (albumin) infusion, endoscopicvariceal banding or sclerotherapy, balloon tamponade, transjugularintrahepatic portosystemic shunt (TIPS), avoiding alcohol, avoidingsedative medicines, such as sleeping pills, anti-anxiety medicines andnarcotics, and avoiding nonsteroidal anti-inflammatory drugs (NSAIDs),such as aspirin, ibuprofen and naproxen. Examples of medications mayinclude GP73 inhibitors, diuretic medicines, such as spironolactone andfurosemide, antibiotics, such as ciprofloxacin, neomycin ormetronidazole (Flagyl), and cefotaxime, beta-blocker medicines, such aspropranolol and nadolol, vasoconstrictor medicines, such as octreotide,lactulose, immunosuppressors, such as prednisone and azathioprine,ursodeoxycholic acid (UDCA; ursodiol (Actigall), colchicine,methotrexate, erythropoietin and epoetin alfa (Epogen, Procrit).

b. Liver Cancer

The subject identified in the methods described above having levels ofGP73 or fucosylated GP73 greater than or equal to the values discussedabove is identified as a patient suffering from liver cancer. Thesubject is then treated for liver cancer. Treatment may include surgicalremoval of the cancer (liver resection) with or without livertransplant, chemotherapy, such as doxorubicin (Adriamycin),5-fluorouracil (5 FU), tamoxifen (Nolvadex), Octreotide (Sandostatin),gemcitabine, cisplatin, and oxaliplatin, biotherapy, such asbevacizumab, chemoembolization (trans-arterial chemoembolization orTACE), radioembolization (selective internal radiotherapy; “SIRT”),ablation, such as radiofrequency ablation (RFA) therapy, percutaneousethanol (alcohol) injection, and cryoablation, stereotacticradiosurgery, and proton beam therapy. Treatment may also include drugsthat block components of the angiogenesis pathway, such as sorafenib(Nexavar). Treatment may also include GP73 inhibitors. The drugs may bedelivered via the hepatic artery or portal vein.

8. TREATMENT OF SUBJECTS SUFFERING FROM CANCER

The subject identified in the methods described above having levels ofGP73 or fucosylated GP73 greater than or equal to the values discussedabove is identified as a patient suffering from cancer. The subject isthen treated for the cancer. Treatment of cancer may include surgery,chemotherapy, radiation therapy, small molecule GP73 or fucosylated GP73inhibitors and/or GP73 or fucosylated GP73 inhibitors.

9. KIT

Provided herein is a kit, which may be used for assaying a test samplefor GP73 or GP73 fragment or fucosylated GP73 or fucosylated GP73fragment. The kit comprises at least one component for assaying the testsample for GP73 or fucosylated GP73 and instructions for assaying thetest sample for GP73 or or fucosylated GP73. For example, the kit cancomprise instructions for assaying the test sample for GP73 or orfucosylated GP73 by immunoassay, e.g., chemiluminescent microparticleimmunoassay. Instructions included in kits can be affixed to packagingmaterial or can be included as a package insert. While the instructionsare typically written or printed materials they are not limited to such.Any medium capable of storing such instructions and communicating themto an end user is contemplated by this disclosure. Such media include,but are not limited to, electronic storage media (e.g., magnetic discs,tapes, cartridges, chips), optical media (e.g., CD ROM), and the like.As used herein, the term “instructions” can include the address of aninternet site that provides the instructions.

The at least one component may include at least one compositioncomprising one or more isolated antibodies or antibody fragments thereofthat specifically bind to GP73 or or fucosylated GP73. The antibody maybe a GP73 or fucosylated GP73 capture antibody and/or a GP73 orfucosylated GP73 detection antibody. The antibody may include the1A-4246 antibody, 1B-4863 antibody, or antibody fragments thereof. Theantibody is optionally detectably labeled.

Alternatively or additionally, the kit can comprise a calibrator orcontrol, e.g., purified, and optionally lyophilized, GP73 or orfucosylated GP73, and/or at least one container (e.g., tube, microtiterplates or strips, which can be already coated with an anti-GP73monoclonal antibody) for conducting the assay, and/or a buffer, such asan assay buffer or a wash buffer, either one of which can be provided asa concentrated solution, a substrate solution for the detectable label(e.g., an enzymatic label), or a stop solution. Preferably, the kitcomprises all components, i.e., reagents, standards, buffers, diluents,etc., which are necessary to perform the assay. The instructions alsocan include instructions for generating a standard curve.

The kit may further comprise reference standards for quantifying GP73 orfucosylated GP73. The reference standards may be employed to establishstandard curves for interpolation and/or extrapolation of GP73 orfucosylated GP73 concentrations. The reference standards may include ahigh GP73 or fucosylated GP73 concentration level, for example, about100 ng/mL, about 125 ng/mL, about 150 ng/mL, about 175 ng/mL, about 200ng/mL, about 225 ng/mL, about 250 ng/mL, about 275 ng/mL, or about 300ng/mL; a medium GP73 concentration level, for example, about 25 ng/mL,about 40 ng/mL, about 45 ng/mL, about 50 ng/mL, about 55 ng/mL, about 60ng/mL, about 75 ng/mL or about 100 ng/mL; and/or a low GP73concentration level, for example, about 1 ng/mL, about 5 ng/mL, about 10ng/mL, about 12.5 ng/mL, about 15 ng/mL, about 20 ng/mL, or about 25ng/mL

Any antibodies, which are provided in the kit, such as recombinantantibodies specific for GP73, can incorporate a detectable label, suchas a fluorophore, radioactive moiety, enzyme, biotin/avidin label,chromophore, chemiluminescent label, or the like, or the kit can includereagents for labeling the antibodies or reagents for detecting theantibodies (e.g., detection antibodies) and/or for labeling the analytesor reagents for detecting the analyte. The antibodies, calibrators,and/or controls can be provided in separate containers or pre-dispensedinto an appropriate assay format, for example, into microtiter plates,

Optionally, the kit includes quality control components (for example,sensitivity panels, calibrators, and positive controls). Preparation ofquality control reagents is well-known in the art and is described oninsert sheets for a variety of immunodiagnostic products. Sensitivitypanel members optionally are used to establish assay performancecharacteristics, and further optionally are useful indicators of theintegrity of the immunoassay kit reagents, and the standardization ofassays,

The kit can also optionally include other reagents required to conduct adiagnostic assay or facilitate quality control evaluations, such asbuffers, salts, enzymes, enzyme co-factors, substrates, detectionreagents, and the like. Other components, such as buffers and solutionsfor the isolation and/or treatment of a test sample (e.g., pretreatmentreagents), also can be included in the kit. The kit can additionallyinclude one or more other controls. One or more of the components of thekit can be lyophilized, in which case the kit can further comprisereagents suitable for the reconstitution of the lyophilized components.

The various components of the kit optionally are provided in suitablecontainers as necessary, e.g., a microtiter plate. The kit can furtherinclude containers for holding or storing a sample (e.g., a container orcartridge for a urine, plasma, or serum sample). Where appropriate, thekit optionally also can contain reaction vessels, mixing vessels, andother components that facilitate the preparation of reagents or the testsample. The kit can also include one or more instrument for assistingwith obtaining a test sample, such as a syringe, pipette, forceps,measured spoon, or the like.

If the detectable label is at least one acridinium compound, the kit cancomprise at least one acridinium-9-carboxamide, at least oneacridinium-9-carboxylate aryl ester, or any combination thereof. If thedetectable label is at least one acridinium compound, the kit also cancomprise a source of hydrogen peroxide, such as a buffer, solution,and/or at least one basic solution. If desired, the kit can contain asolid phase, such as a magnetic particle, bead, test tube, microtiterplate, cuvette, membrane, scaffolding molecule, film, filter paper,disc, or chip.

If desired, the kit can further comprise one or more components, aloneor in further combination with instructions, for assaying the testsample for another analyte, which can be a biomarker, such as abiomarker of liver disease or disorder.

a. Adaptation of Kit and Method

The kit (or components thereof), as well as the method for determiningthe concentration of GP73 or fucosylated GP73 in a test sample by animmunoassay as described herein, can be adapted for use in a variety ofautomated and semi-automated systems (including those wherein the solidphase comprises a microparticle), as described, e.g., in U.S. Pat. Nos.5,089,424 and 5,006,309, and as commercially marketed, e.g., by AbbottLaboratories (Abbott Park, Ill.) as ARCHITECT®.

Some of the differences between an automated or semi-automated system ascompared to a non-automated system (e.g., ELISA) include the substrateto which the first specific binding partner (e.g., analyte antibody orcapture antibody) is attached (which can affect sandwich formation andanalyte reactivity), and the length and timing of the capture,detection, and/or any optional wash steps. Whereas a non-automatedformat such as an ELISA may require a relatively longer incubation timewith sample and capture reagent (e.g., about 2 hours), an automated orsemi-automated format (e.g., ARCHITECT® and any successor platform,Abbott Laboratories) may have a relatively shorter incubation time(e.g., approximately 18 minutes for ARCHITECT®). Similarly, whereas anon-automated format such as an ELISA may incubate a detection antibodysuch as the conjugate reagent for a relatively longer incubation time(e.g., about 2 hours), an automated or semi-automated format (e.g.,ARCHITECT® and any successor platform) may have a relatively shorterincubation time (e.g., approximately 4 minutes for the ARCHITECT® andany successor platform).

Other platforms available from Abbott Laboratories include, but are notlimited to, AxSYM®, IMx® (see, e.g., U.S. Pat. No. 5,294,404, which ishereby incorporated by reference in its entirety), PRISM®, EIA (bead),and Quantum™ II, as well as other platforms. Additionally, the assays,kits, and kit components can be employed in other formats, for example,on electrochemical or other hand-held or point-of-care assay systems.The present disclosure is, for example, applicable to the commercialAbbott Point of Care (i-STAT®, Abbott Laboratories) electrochemicalimmunoassay system that performs sandwich immunoassays. Immunosensorsand their methods of manufacture and operation in single-use testdevices are described, for example in, U.S. Pat. No. 5,063,081, U.S.Pat. App. Pub. No. 2003/0170881, U.S. Pat. App. Pub. No. 2004/0018577,U.S. Pat. App. Pub. No. 2005/0054078, and U.S. Pat. App. Pub. No.2006/0160164, which are incorporated in their entireties by referencefor their teachings regarding same.

In particular, with regard to the adaptation of an assay to the I-STAT®system, the following configuration is preferred. A microfabricatedsilicon chip is manufactured with a pair of gold amperometric workingelectrodes and a silver-silver chloride reference electrode. On one ofthe working electrodes, polystyrene beads (0.2 mm diameter) withimmobilized capture antibody are adhered to a polymer coating ofpatterned polyvinyl alcohol over the electrode. This chip is assembledinto an I-STAT® cartridge with a fluidics format suitable forimmunoassay. On a portion of the wall of the sample-holding chamber ofthe cartridge there is a layer comprising the detection antibody labeledwith alkaline phosphatase (or other label). Within the fluid pouch ofthe cartridge is an aqueous reagent that includes p-aminophenolphosphate.

In operation, a sample suspected of containing GP73 or fucosylated GP73is added to the holding chamber of the test cartridge and the cartridgeis inserted into the I-STAT® reader. After the second antibody(detection antibody) has dissolved into the sample, a pump elementwithin the cartridge forces the sample into a conduit containing thechip. Here it is oscillated to promote formation of the sandwich betweenthe first capture antibody, GP73 or fucosylated GP73, and the labeledsecond detection antibody. In the penultimate step of the assay, fluidis forced out of the pouch and into the conduit to wash the sample offthe chip and into a waste chamber. In the final step of the assay, thealkaline phosphatase label reacts with p-aminophenol phosphate to cleavethe phosphate group and permit the liberated p-aminophenol to beelectrochemically oxidized at the working electrode. Based on themeasured current, the reader is able to calculate the amount of analyteGP73 or fucosylated GP73 in the sample by means of an embedded algorithmand factory-determined calibration curve.

The methods and kits as described herein necessarily encompass otherreagents and methods for carrying out the immunoassay. For instance,encompassed are various buffers such as are known in the art and/orwhich can be readily prepared or optimized to be employed, e.g., forwashing, as a conjugate diluent, and/or as a calibrator diluent. Anexemplary conjugate diluent is ARCHITECT® conjugate diluent employed incertain kits (Abbott Laboratories, Abbott Park, Ill.) and containing2-(N-morpholino)ethanesulfonic acid (MES), a salt, a protein blocker, anantimicrobial agent, and a detergent. An exemplary calibrator diluent isARCHITECT® human calibrator diluent employed in certain kits (AbbottLaboratories, Abbott Park, Ill.), which comprises a buffer containingMES, other salt, a protein blocker, and an antimicrobial agent.Additionally, as described in U.S. Pat. Application No. 61/142,048 filedDec. 31, 2008, improved signal generation may be obtained, e.g., in anI-STAT® cartridge format, using a nucleic acid sequence linked to thesignal antibody as a signal amplifier.

While certain embodiments herein are advantageous when employed toassess disease, such as liver disease or cancer, the assays and kitsalso optionally can be employed to assess GP73 in other diseases,disorders, and conditions as appropriate.

The method of assay also can be used to identify a compound thatameliorates diseases, such as liver disease or cancer. For example, acell that expresses GP73 can be contacted with a candidate compound. Thelevel of expression of GP73 or fucosylated GP73 in the cell contactedwith the compound can be compared to that in a control cell using themethod of assay described herein.

The present invention has multiple aspects, illustrated by the followingnon-limiting examples.

10. EXAMPLES

It will be readily apparent to those skilled in the art that othersuitable modifications and adaptations of the methods of the presentdisclosure described herein are readily applicable and appreciable, andmay be made using suitable equivalents without departing from the scopeof the present disclosure or the aspects and embodiments disclosedherein. Having now described the present disclosure in detail, the samewill be more clearly understood by reference to the following examples,which are merely intended only to illustrate some aspects andembodiments of the disclosure, and should not be viewed as limiting tothe scope of the disclosure. The disclosures of all journal references,U.S. patents, and publications referred to herein are herebyincorporated by reference in their entireties.

The present invention has multiple aspects, illustrated by the followingnon-limiting examples.

Example 1 Human GP73 Gene Expression

Human GP73 protein (aka GOLM1, GOLPH2) is a 400 amino acid (45.2 kDa)integral protein of the cis-Golgi membrane. The human GP73 (amino acids63 to 400 based on GenBank sequence AAF44663.1 (SEQ ID NO:97) wassynthesized and cloned into a pET vector (Novagen) for expression. A6×His Tag was fused at the C-terminal for purification. The finalplasmid pET-GP73 (SEQ ID NO:98, see FIG. 1) was transformed intoBL21(DE3) cell and induced by 1 mM IPTG at 30° C. for 4 hrs. The inducedE. coli cells were centrifuged for 20 min at 3,900 rpm and washed oncewith PBS. The washed cell pellet was re-suspended with about 5 mLBugBuster® Protein Extraction reagent (Novagen) per gram cell pellet.Cell suspensions were incubated for 30 min at room temperature withgentle shaking. The lysates were centrifuged at 14,000 rpm for 20 min at4° C. The supernatant was purified using nickel affinity columnchromatography per manufacturer instruction (Novagen).

In addition, the washed cell pellet was dissolved in 1×His-bind bindingbuffer (Novagen) with protease inhibitors (Sigma) and sonicated in icebath for 5×10 seconds pulse with 30 seconds rest between pulses. Thelysates were centrifuged at 14,000 rpm for 20 min at 4° C. Thesupernatant was purified using nickel affinity column chromatography permanufacturer instruction (Novagen).

The human GP73 (amino acids 63 to 400) was also cloned into thepFuse-hIgG1-Fc vector (InvivoGen). A human Fc region was fused atC-terminal to generate GP73-hFc (GP73 linked to the fragment crystallineregion of human IgG; SEQ ID NO:99, see FIG. 2). The final plasmid DNApFuse-GP73-Fc was transfected with linear polyethyleneimine (PEI, MW 25kDa) (Polysciences, Inc.) into HEK293F, CHO or HepG2 cells. After 5 daysof culture, the supernatant was harvested by centrifugation at 1,000 rpmfor 20 min and followed by filtration (0.2 micron filter). The harvestedsupernatant was purified by Protein G column chromatography permanufacturer instruction (GE healthcare).

Example 2 Animal Immunizations

Female CAF1/J and RBF/DnJ mice (both from The Jackson Laboratory, BarHarbor, Me.) were immunized twice, every seven weeks, with 20 mgpurified GP73-hFc (described in Example 1) emulsified in Complete orIncomplete Adjulite Freund's Adjuvant (Pacific Immunology, Ramona,Calif.). Complete Freund's adjuvant was used for the primaryimmunization and Incomplete Freund's adjuvant was used for the secondimmunization. Each inoculum was prepared by first diluting the GP73-hFcto the appropriate concentration in sterile saline (0.9% sodiumchloride, Abbott Laboratories), adding an equal volume of adjuvant andthen mixing by passing back and forth between two syringes via a 3-waystopcock until a thick, stable emulsion was formed. Sera samples weretaken 10-14 days following the second immunization. On the fourth andthird days prior to B cell harvest, a RBF/DnJ mouse was administered 35μg GP73-hFc diluted in sterile saline. This inoculum was delivered intothe body cavity near the spleen. On the third day prior to B cellharvest, a CAF1/J mouse was administered 50 μg GP73-hFc diluted insterile saline. This inoculum was also delivered into the body cavitynear the spleen.

Example 3 Screening of Mouse Sera for Antigen Reactivity

Sera samples were tested in a 96-well micro titer enzyme immunoassay(EIA) for reactivity to solution phase GP73-hFc. Assay plates (NUNCCorporation, Naperville, Ill.) were coated with 100 μL/well of sheepanti-mouse IgG Fc specific antibody (Jackson ImmunoResearch, West Grove,Pa.) diluted to 2 μg/mL in phosphate-buffered saline (PBS, AbbottLaboratories). Plates were incubated overnight, the capture antibody wasremoved, and 200 μL/well of blocking solution was added (3% w/v[weight/volume] bovine serum albumin (BSA) and 0.5% v/v [volume/volume]polysorbate-20 diluted in PBS (all Abbott Laboratories)). The plateswere incubated for about 30 min and then washed with distilled water(dH₂O, Abbott Laboratories). Serial dilutions (in block solution) of themouse sera or a positive control were added to the assay plates (100μL/well), incubated for about 60 min and then washed with dH₂O. 100μL/well of normal serum solution (NSS; block solution containing 2% v/vnormal mouse serum) was added for additional blocking. This solutionhelps to prevent non-specific binding in the assay well. The plates wereincubated for about 30 min and then washed with dH₂O, Subsequently, 100μL/well of a 500 ng/mL solution of GP73-hFc was added to the assay wellsfor a brief incubation, after which the plates were washed with dH₂O.Rabbit anti-GP73 antibody (Drexel University) diluted to 500 ng/mL inNSS was added to all assay wells (100 μL/well), which were incubated for30 min and then washed with dH₂O, Next, 100 μL horseradish peroxidaselabeled goat anti-rabbit (Jackson ImmunoResearch) diluted to 200 ng/mLin NSS was added, allowed to incubate for about 30 min and then theplates washed. o-Phenylenediamine (OPD) substrate (Abbott Laboratories)was used as the chromagen to generate signal, and the reaction wasquenched using 1 N sulfuric acid (Abbott Laboratories). Signal was readat a wavelength of 492 nm.

Example 4 Screening of Mouse Sera for Relative Affinity

Samples were tested for reactivity to limiting concentrations of antigento determine the relative affinity of each sera sample for the GP73-hFcantigen. The assay format was identical to that described above, exceptthat instead of preparing serial dilutions of mouse sera test samples,each sample was prepared at a single dilution, in block. Additionally,the GP73-hFc antigen was tested at varying concentrations, beginningwith 2000 ng/mL in NSS, followed by ten log 3 dilutions, also in NSS.Binding curves were generate and used to determine relative affinity.Based on these results, a CAF1/J mouse (“mouse #458”) and a RBF/DnJmouse (“mouse #501”) were chosen for B cell fusion.

Example 5 Mouse Splenocyte Fusion

On the day of fusion, the mice were euthanized and their spleenscontaining anti-GP73 splenocytes were harvested and placed intoHybridoma Serum Free Medium (HSFM) supplemented with Pen Strep(Invitrogen Corporation). A cell fusion was performed as described byKohler and Milstein (Nature (1975) 256:495-7). Each mouse spleen wasplaced into a separate petri dish containing HSFM. The splenocytes wereperfused out of each spleen using a syringe containing HSFM and cellscraper, then counted using a hemocytometer. Approximately 3.9×107splenocytes from mouse #458 was isolated in a 50 mL centrifuge tube forfusion 1A, and 3.8×107 splenocytes from mouse #501 was isolated in a 50mL centrifuge tube for fusion 1B. Splenocytes from each mouse werewashed by centrifugation into a cell pellet and re-suspended in HSFM.These splenocytes were mixed with an equal number of NS/0 myeloma cells(American Type Culture Collection) and centrifuged into a pellet. Thefusion was accomplished by exposing the splenocytes and NS/0 cells to50% Polyethylene glycol (PEG) (MW 1300-1600) in HSFM. One mL of the PEGsolution was added to each cell pellet over 30 seconds, followed by oneadditional minute of incubation. The PEG and cell pellet were diluted byslowly adding 30 mL of HSFM over 30 seconds. The fused cells were thenremoved from suspension by centrifugation and decanting the supernatant.The cell pellet from each fusion was re-suspended into approximately 300mL of HSFM supplemented with approximately 10% fetal bovine serum (FBS)(Hyclone Laboratories), HAT (Hypoxanthine, Aminopterin, Thymidine)(Sigma Laboratories), HT Supplement (Invitrogen Corporation), BMCondimed H1 (Roche Applied Science), Cholesterol and L-Glutamine(Invitrogen Corporation) to select for hybridomas. The fused cells wereseeded into T162 culture flasks at an approximate density of 2.5×10⁵cells/mL and cultured in bulk for approximately 48 hrs at 37° C. with 5%CO2. Following 48 hrs of HAT selection, the bulk culture was centrifugedand the pellet was re-suspended into semi-solid tissue culture medium.The semi-solid tissue culture medium consisted of a 50% mixture of2×IMDM (Invitrogen) with CloneMatrix (Molecular Devices) supplementedwith 10% FBS, HT Supplement, Penn/Strep, L-Glutamine, and a 5 μg/mLsolution of CloneDetect (Molecular Devices). The semi-solid cultureplates were allowed to incubate for 7-10 days before colony selection onthe ClonepixFL (Molecular Devices). A colony grown in the semi-solidmedium was considered a clone because the single cell initiating it hadnot been allowed to move and mix with other cells during growth. Allcell lines of interest were subcloned to ensure clonality. Animmunoprecipitation reaction occurs between the antibody being producedby the colony and the goat anti-mouse IgG Fc-FITC that fluoresces. Thebrighter the fluorescence signal observed, the more antibody beingproduced. Colonies were analyzed for fluorescence on the ClonepixFL andthe ones with the brightest fluorescent signal were selected forautomated transfer to 96 well tissue culture plates containing HSFM with10% FBS and L-Glutamine. The 96 well tissue culture plates were allowedto grow for 3 to 5 days at 37° C. prior to supernatant screening forantibody production.

Example 6 Hybridoma Screening and Selection

Cell supernatant samples were analyzed for anti-GP73 antibodies by EIA.Sheep anti-mouse IgG Fc (Jackson Immunoresearch) was coated on 96 wellmicro-titer EIA plates at 1 μg/mL. After the capture reagent was coatedon the solid phase, it was removed and the plates were blocked using aBSA/PBS block solution. The wells were washed with distilled water andcell supernatants were added to the blocked plates and allowed toincubate at room temperature for at least one hour. A mouse monoclonalantibody 14H4-23 (Drexel University) was used a positive control. Theanti-mouse IgG Fc captured the anti-GP73 mouse antibody from thesupernatant. Following the incubation, the supernatants were washed offusing distilled water. An irrelevant mouse-human chimeric antibody wasadded to all wells and incubated at room temperature for 30 min to blockany captured mouse antibodies that react with the human IgG Fc fusionprotein linked to the GP73 antigen. The wells were washed with distilledwater and GP73-hFc was added to the plates at 200 ng/mL and incubatedfor 30 min. Following this incubation, the antigen was washed from theplates using distilled water. Biotin labeled goat anti-GP73 was added tothe plates at 200 ng/mL and incubated for 30 min at room temperature.The plates were washed and streptavidin-HRPO (Jackson Immunoresearch)(diluted to approximately 200 ng/mL) was added to the plates and allowedto incubate for 30 min. The plates were washed with distilled water andOPD substrate was used as the chromagen to generate signal. Plates wereread at 492 nm and the results were analyzed. Hybrids were consideredpositive if they had an EIA signal at least 3 times greater thanbackground. See Table 3.

TABLE 3 Sample OD (A492) Background 0.06 PC (14H4-23) 1.57 1A-3187 1.341A-4246 1.92 1B-3246 1.48 1B-3440 1.46 1B-4863 1.44 1B-4971 1.79

Positive clones were expanded to 24 well plates in IMDM supplementedwith 10% FBS and HT supplement. Following 5-14 days growth, the 24 wellcultures were evaluated by EIA in the same manner as previouslydescribed, except the supernatant samples were titrated against GP73-hFcand BSA to eliminate non-specific protein binders. Once again the clonesgenerating signal at least 3 times greater than background wereconsidered positive and selected for further evaluation. See Table 4.

TABLE 4 BSA Background GP73-hFc OD Sample Dilution OD (A492) (A492) NCmAb 400 ng/mL 0.05 0.05 PC (14H4-23) 400 ng/mL 0.06 1.49 1A-3187 1:250.11 1.30 1A-4246 1:25 0.04 1.35 1B-3246 1:16 0.06 1.64 1B-3440 1:250.09 1.51 1B-4863 1:25 0.06 1.61 1B-4971 1:25 0.10 2.10

The 24 well cultures were then evaluated by EIA for their ability toform a sandwich with the rabbit anti-GP73 used as a capture reagentcoated directly on the solid phase. The same EIA protocol previouslydescribed was used, except rabbit anti-GP73 antibody was coated at 1μg/mL, the GP73-hFc was tested at 200 ng/mL, and a HRP labeled goatanti-mouse IgG FC antibody was used as the conjugate reagent to generatesignal. See Table 5.

TABLE 5 BSA Background GP73-hFc OD Sample Dilution OD (A492) (A492) NCmAb 400 ng/mL 0.10 0.10 PC (14H4-23) 400 ng/mL 0.10 1.34 1A-3187 1:1250.09 0.97 1A-4246 1:25  0.12 0.85 1B-3246 1:125 0.10 0.79 1B-3440 1:1250.12 0.76 1B-4863 1:125 0.10 1.41 1B-4971 1:125 0.10 1.60

The clones that were identified as positive at the 24 well stage wereexpanded for cryopreservation followed by generation of high-densitycell supernatant. Supernatant from these clones were tested for mouseantibody isotype using a Southern Biotech Clonotyping Kit in an EIAformat. See Table 6.

TABLE 6 Sample Isotype 1A-3187 IgG1k 1A-4246 IgG1k 1B-3246 IgG1k 1B-3440IgG1k 1B-4863 IgG1k 1B-4971 IgG1k

Example 7 Hybridoma Scale Up, Antibody Purification, and AntibodyLabeling

The cell lines were expanded in IMDM (Invitrogen Corporation)supplemented with L-glutamine and 10% Ultra Low IgG FBS (InvitrogenCorporation) and seeded into roller bottles at approximately 0.5×10⁵cells/mL The cultures were incubated at 37° C. while rotating atapproximately 1 revolution per minute for 10-14 days, or until aterminal end culture was obtained. The terminal roller bottlesupernatant was harvested and clarified with a 0.45 micron filter. Theclarified supernatant was diluted with an equal volume of 1.5 Mglycine/3 N NaCl buffer at pH 8.9 (Abbott Laboratories), then loadedonto a pre-equilibrated 5 mL Protein A column using the AKTA automatedpurification system (Amersham/Pharmacia/GE). The column was then washedwith approximately 5 column volumes of binding buffer and when a stablebaseline was achieved, the mAb was eluted with a pH 3.0 citrate buffer(Abbott Laboratories). The mAb was then transferred to a desaltingcolumn for an exchange into PBS, and then further dialyzed in PBS using10,000 molecular weight cut-off dialysis membrane (Pierce Chemical). Thepurified antibodies were biotin labeled for use as secondary screeningreagents. Sulfo-NHS-LC-Biotin (Pierce) was added to purified antibody ata 20 molar excess and allowed to incubate for 30 min. Unbound biotin wasremoved through dialysis in PBS and the mAbs were tested by EIA toconfirm they were successfully labeled.

Example 8 Purified Antibody Characterization

1. Sensitivity to Fucose—Competitive Inhibition Assay with AleuriaAurantia Lectin (AAL)

The biotin labeled anti-GP73 mAbs were tested to determine if they weresensitive to the presence of fucose on the GP73-hFc antigen by EIA.Aleuria Aurantia Lectin (AAL) (Vector Laboratories) binds fucose with ahigh degree of specificity. Donkey anti-human IgG Fc (JacksonImmunoresearch) that was pretreated with periodate to oxidize anyglycosylation/fucosylation sites was coated on 96 well micro titer EIAplates at approximately 1 μg/mL Periodate treatment was done to preventnonspecific binding of AAL to the capture reagent. After the capturereagent was coated on the solid phase, the capture reagent was removedand the plates were blocked for 30 min using Protein-Free T20 (TBS)Blocking Buffer (Thermo Scientific). The wells were washed withdistilled water and a 200 ng/mL solution of GP73-hFc antigen was addedto the blocked plates and allowed to incubate at room temperature for atleast 30 min. The anti-human IgG Fc captures the human IgG fusionprotein on the GP73 antigen. The wells were washed with distilled waterand AAL was added to the wells in serial dilutions from 0-200 ng/mL andincubated for at least 30 min. Following this incubation, the antigenwas washed from the plates using distilled water. Biotin labeledanti-GP73 mAbs were added to the plates at predetermined concentrationsfor each mAb ranging from 50 to 2000 ng/mL and incubated for 15 min atroom temperature. Biotin labeled AAL was used as a positive control forthe assay and a biotin labeled irrelevant mAb was used as the negativecontrol. The plates were washed and streptavidin-HRPO (diluted toapproximately 200 ng/mL) was added to the plates and allowed to incubatefor 30 min. The plates were washed with distilled water and OPDsubstrate was used as the chromagen to generate signal. Plates were readat 492 nm and the results were analyzed. See FIG. 3. As shown in FIG. 3,biotin labeled anti-GP73 mAbs 1A-3187 and 1B-4863 demonstrate somecompetitive inhibition from free AAL at the higher concentrations testedin this assay. All other biotin labeled mAbs exhibit no inhibition evenat 200 ng/mL

2. Reactivity with IgG, GP73, and GP73-hFc

The purified anti-GP73 mAbs were tested for EIA reactivity to human IgG,GP73 produced in E. coli, and GP73-hFc produced in HEK 293 cells. Amouse-human chimeric antibody, GP73 produced in E. coli, and GP73-hFcproduced in HEK 293 cells were coated on micro titer plates atapproximately 1000 ng/mL and allowed to incubate over night at 4-8° C.After the capture reagent was coated on the solid phase, it was removedand blocked using a BSA/PBS block solution. The wells were washed withdistilled water. Purified anti-GP73 and negative control mAbs were addedto the blocked plates in serial dilutions starting at 1000 ng/mL andallowed to incubate at room temperature for approximately 30 min. Thewells were washed with distilled water. Goat anti-mouse IgG-HRP (JacksonImmunoresearch) was added to the wells at approximately 250 ng/mL andallowed to incubate for approximately 30 min at room temperature. Theplates were washed with distilled water and OPD substrate was used asthe chromagen to generate signal. Plates were read at 492 nm and theresults were analyzed. See FIG. 4. As shown in FIG. 4, none of the mAbstested react with the human IgGFc on the mouse-human chimeric and allmAbs tested demonstrate reactivity with GP73-hFc. The 1A-3187 and1A-4246 demonstrate no reactivity with the E. coli produced GP73 withoutthe human IgG Fc fusion protein suggesting this was structurallydifferent than the GP73-hFc, possibly due to the lack of glycosylationfrom the E. coli production organism.

3. Fucose Sensitivity—Free Fucose Inhibition

The biotin labeled anti-GP73 mAbs were tested to determine if they weresensitive to the presence of fucose on the GP73-hFc antigen by EIA.Aleuria Aurantia Lectin (AAL) (Vector Laboratories) binds fucose with ahigh degree of specificity. Donkey anti-human IgG Fc (JacksonImmunoresearch) was coated on 96 well micro titer EIA plates atapproximately 1 μg/mL. After the capture reagent was coated on the solidphase, the capture reagent was removed and the plates were blocked for30 min using Protein-Free T20 (TBS) Blocking Buffer (Thermo Scientific).The wells were washed with distilled water. A 200 ng/mL solution ofGP73-hFc antigen was added to the blocked plates and allowed to incubateat room temperature for at least 45 min then washed with distilledwater. Fucose was added to the wells of a separate non-absorbingmicrotiter plate in serial dilutions starting from 1000 μg/mL Biotinlabeled anti-GP73 mAbs were added to the plates at predeterminedconcentrations for each mAb ranging from 50 to 2000 ng/mL and incubatedfor 45 min at room temperature. The mixture was added to the microtiterplates with the captured GP73-hFc and allowed to incubate for 15-20 minat room temperature. Biotin labeled AAL was used as a positive controlfor the assay and a biotin labeled irrelevant mAb was used as thenegative control. The plates were washed. Streptavidin-HRPO (diluted toapproximately 200 ng/mL) was added to the plates and allowed to incubatefor 30 min. The plates were washed with distilled water and OPDsubstrate was used as the chromagen to generate signal. Plates were readat 492 nm and the results were analyzed. See FIG. 5. FIG. 5 indicatesthat biotin labeled AAL binding was inhibited by free fucose, but noneof the biotin labeled anti-GP73 mAbs demonstrated any inhibition fromfree AAL. These results indicate that none of the GP73 mAbs bindspecifically to fucose alone in solution.

4. Reactivity to Periodate and No-Periodate Treated GP73-hFc

The biotin labeled mAbs were further evaluated to compare theirreactivity to periodate and non-periodate treated GP73-hFc antigen.Periodate treatment should oxidize the glycosylation/fucosylation siteson the antigen so an antibody reactive with fucose should not be asreactive with the periodate treated material. Donkey anti-human IgG Fc(Jackson Immunoresearch) that was pretreated with periodate to oxidizeany glycosylationducosylation sites was coated on 96 well micro titerEIA plates at approximately 1 μg/mL Periodate treatment was done toprevent nonspecific binding of fucose reactive antibodies with thecapture reagent. After the capture reagent was coated on the solidphase, it was removed and the plates were blocked for 30 min at roomtemperature using Protein-Free T20 (TBS) Blocking Buffer (ThermoScientific). The wells were washed with distilled water. Serialdilutions of both the periodate treated and non-periodate treatedGP73-hFc antigen starting at 100 ng/mL were added to the blocked platesand allowed to incubate at room temperature for at least 30 min. Theplates were washed. Streptavidin-HRPO (diluted to approximately 200ng/mL) was added to the plates and allowed to incubate for 30 min atroom temperature. The plates were washed with distilled water and OPDsubstrate was used as the chromagen to generate signal. As shown inFIGS. 6-11, antibodies 1A-3187, 1B-4863, and 1B-4971 exhibited reducedbinding to periodate treated GP73-hFc compared to the non-periodatetreated material.

5. Binding Pairs

The purified anti-GP73 mAbs were tested for their ability to formbinding pairs with GP73-hFc antigen by EIA. Anti-GP73 producedantibodies were coated on micro titer plates at approximately 1000 ng/mLand allowed to incubate over night at 4-8° C. After the capture reagentwas coated on the solid phase, the plates were blocked using a BSA/PBSblock solution. The wells were washed with distilled water and purifiedGP73-hFc was added to the blocked plates in serial dilutions from 0-100ng/mL diluted in BSA block and allowed to incubate at room temperaturefor approximately 30 min. The wells were washed with distilled water andbiotin labeled anti-GP73 mAbs were added to the plates at concentrationspredetermined for each mAb ranging from 50 to 5000 ng/mL and incubatedfor 30 min at room temperature. The plates were washed.Streptavidin-HRPO (diluted to approximately 200 ng/mL) was added to theplates and allowed to incubate for 30 min at room temperature. Theplates were washed with distilled water and OPD substrate was used asthe chromagen to generate signal. Plates were read at 492 nm and theresults were analyzed. Table 7 summarizes the assay signal for eachantibody pair combination, which indicates whether or not each bindingpair was capable of forming a sandwich. These antibodies were groupedinto 6 different epitopes based on their sandwich formation pattern.Antibodies that did not form a sandwich, or formed a weak sandwich, wereassigned to the same epitope group. It was assumed that antibodiesbinding to the same or similar epitope could not form a GP73 sandwichtogether because they competed for the same binding site. There wereexceptions to this for cases like antibody 1B-4863, which was capable offorming a sandwich with all antibodies, including the biotin labeledversion of itself. Because there were two GP73 molecules attached to onehuman IgG Fc it was possible for an antibody to sandwich with itself,but for most of these mAbs, binding of the first antibody seemed toblock the second epitope present on the same GP73-hFc molecule andprevent another antibody directed against the same epitope from binding.

TABLE 7 1B- 1B- 1A- 1B- 1A- 1B- NC- Coated 3246-Bt 3440-Bt 4246-Bt4971-Bt 3187-Bt 4863-Bt Bt 1B- 0.47 3.20 2.52 3.04 3.06 2.94 0.06 32461B- 3.12 0.42 3.10 3.45 3.47 3.09 3.07 3440 1A- 3.32 3.68 0.10 4.65 4.083.50 0.08 4246 1B- 1.78 1.62 1.18 0.63 1.57 1.42 0.06 4971 1A- 1.03 0.850.52 0.86 0.29 1.08 0.06 3187 1B- 2.61 2.85 2.52 3.27 2.90 2.41 0.064863 NC 0.05 0.05 0.05 0.06 0.09 0.05 0.06

6. Western Blot Analysis Using Anti-GP73 Antibody

The mammalian cell (HEK and CHO) expressed GP73-hFc or E. coli expressedGP73, as described above, were electrophoresed in a 4-20% SDS-PAGE gel.The GP73 Proteins were transferred to nitrocellulose membranes at 100volts for 1-2 hrs in a standard transfer buffer (25 mM Tris, 192 mMglycine, and 20% methanol, pH 8.3). The nitrocellulose membrane wasblocked with PBS buffer containing 5% non-fat dry milk and 0.05% Tween20 for about 1 hr. The nitrocellulose membrane was incubated with anappropriate amount of anti-GP73 monoclonal antibody in 10 mL of 2.5%non-fat dry milk and 0.05% Tween 20 PBS buffer, pH 7.2. Thenitrocellulose membranes were washed with 0.05% Tween 20/PBS, pH 7.2,incubated with goat anti-mouse IgG antibody conjugated with HRP for 1 hrat room temperature, and washed with 0.05% Tween 20/PBS buffer. Antibodybound to the GP73 protein was visualized by the addition of freshlyprepared metal enhanced 3,3′-Diaminobenzidine in stable peroxide buffer(Pierce, Ill.). See Table 8.

TABLE 8 Western blot on Western blot on Western blot HEK expressed CHOexpressed on E. coli GP73-hFc GP73-hFc expressed Antibody antigenantigen GP73 antigen anti-GP73 1B-3246 Positive Positive Positiveanti-GP73 1A-4246 Positive Positive Negative anti-GP73 1A-3187 PositivePositive Negative anti-GP73 1B-4863 Positive Positive Positive anti-GP731B-3440 Positive Positive Not done anti-GP73 1B-3263 Positive PositiveNot done GP73 14H4-23-288 Positive Not done Positive

Summary of the antibody characterization is shown in Tables 9 and 10.

TABLE 9 Clone # Isotype W. Blot CHO GP73 W. Blot HEK GP73 1A-3187lgG1k + + 1A-4246 lgG1k + + 1B-3246 lgG1k + + 1B-3440 lgG1k + + 1B-4863lgG1k + + 1B-4971 lgG1k + + 14H4-23 N/A + +

TABLE 10 Binds Binds Periodated Free AAL E. coli Hu Free Fucose GP73Clone # Displacement GP73 IgG Displacement reactivity 1A-3187 Yes No NoNo Reduced 1A-4246 No No No No Strong 1B-3246 No Yes No No Strong1B-3440 No Yes No No Strong 1B-4863 Yes Yes No No Reduced 1B-4971 No YesNo No Reduced 14H4-23 No Yes No No Strong

Example 9 Anti-GP73 Monoclonal Antibody Gene Sequencing

The mRNA was extracted from appropriate hybridoma cell cultures usingcommercially available reagents (Oligotex direct mRNA kit, Qiagen)following the manufacturer's recommendations. The heavy chain and kappalight chain cDNA was generated from the extracted mRNA using SuperscriptIII (Life Technologies) and oligo dT primers (Novagen) followingstandard protocols. Amplicons were cloned into a commercially availablevector (Life technologies) per the manufacturer's directions andtransformed into E. coli. Sequence analysis using M13 forward andreverse primers was performed using BigDye Terminator v3.1 cyclesequencing kit (Applied Biosystems, Foster City, Calif.) on plasmidsisolated from multiple transformed E. coli colonies to identify theheavy and light chain coding sequences. See Tables 1 and 2. FIGS. 12-17show a diagram and the amino acid and nucleotide sequence of thevariable heavy and light regions with the CDR sequences identified.

The 1A-3187 variable light (VL) sequence uses a V segment of the IGKV21subgroup. The 1A-4246 and 1B-3440 VL sequences use a V segment of theIGKV4/5 subgroup. The 1B-4863 VL sequence uses a V segment of theIGKV19/28 subgroup. The 1B-3246 VL sequence uses a V segment of theIGKV24/25 subgroup. The 1B-4971 VL sequence uses a V segment of theIGKV2 subgroup.

The 1A-3187 variable heavy (VH) sequence uses a V segment of theIgh-VJ558 VH1 family. The first amino acid after CDR-H2 varies from thetypical antibody sequences near CDRs having a T instead of K/R. The1B-4971 VH sequence also uses a V segment of the Igh-VJ558 VH1 family.The 1B-3440 VH sequence uses a V segment of the IgH-V10 VH10 family. The1B-4863 VH sequence uses a V segment of the Igh-VQ52 VH2 family. The1A-4246 and 1B-3246 VH sequences use a V segment of the Igh-V7183 VH5family.

Example 10 GP73 Epitope Mapping with Anti-GP73 MAb

Epitope mapping of GP73 with anti-GP73 MAb was completed using epitopeexcision followed by LC/MS/MS method. The samples used were GP73-hFcantigen and anti-GP73 MAb

1. Immobilization of anti-GP73 MAb to the CNBr-Activated Sepharoseresin: 20 mg of CNBr-Activated Sepharose resin was weighted into acompact reaction column with frit. The resin was washed three times with200 μL of 1 mM HCl. The resin was washed three times with 200 μL of 100mM NaHCO₃ pH 8/500 mM NaCl buffer. Anti-GP73 MAb was added to thecolumn. The reaction column was placed on a rotator at room temperaturefor 4 hrs. The resin was washed three times with 200 μL of 100 mM NaHCO₃pH 8/500 mM NaCl buffer. 200 μL of 100 mM Tris-HCl pH 8/500 mM NaCl wasadded to the column and the column was rotated at room temperature for 1hr. The resin was washed three times with 200 μl of 100 mM sodiumacetate pH 4/500 mM NaCl buffer and 100 mM Tris-HCl pH 8/500 mM NaClbuffer. The resin was washed three times with 200 μL of 100 mM NaHCO₃ pH8/100 mM NaCl buffer. 200 μL of 100 mM NaHCO₃ pH 8/100 mM NaCl bufferwas added and the top cap on the column was secured.

2. Antigen Binding: The reaction column was washed three times with 200μL of 100 mM NaHCO₃ pH 8/500 mM NaCl buffer. 50 μL antibody resinprepared in step 1 was transfer to the new reaction column. The resinwas washed three times with 200 μL of 100 mM NaHCO₃ pH 8/100 mM NaClbuffer. GP73-hFc antigen sample was added to the column. The reactioncolumn was placed on a rotator at room temperature for 4 hrs. The resinwas washed three times with 200 μl of 100 mM NaHCO₃ pH 8/100 mM NaClbuffer.

3. Proteolysis: 200 μL of 100 mM NaHCO₃ pH 8/100 mM NaCl buffer wasadded to the column. Trypsin was added to the column and the reactioncolumn was placed on a rotator at room temperature overnight.

4. Elution: Solution was flushed through and collected as flow-throughfraction. The resin was washed three times with 200 μL of 100 mM NaHCO₃pH 8/100 mM NaCl buffer twice. The resin was washed three times with 200μL of 100 mM NaHCO₃ pH 8/500 mM NaCl buffer. The resin was washed threetimes with 200 μL of 100 mM NaHCO₃ pH 8/100 mM NaCl buffer. 200 μL of 2%formic acid was added for elution. The eluted fraction and otherfractions were dried with an Eppendorf Vacufuge.

5. C18 ziptip desalting of samples before LC/MS/MS injection: The elutedfractions were reconstituted in 10 μL 0.1% trifluoroacetic acid (TFA).The C18 ziptip was wet three times with 50% ACN/H₂O. The C18 ziptip waswet three times with 0.1% TFA. The samples were aspirated and dispensed15 times for binding. The tip was washed 7 times with 0.1% TFA. Thesamples were eluted with 10 μL 50% ACN/H₂O, 0.1% TFA.

6. The eluted fraction was analyzed by LC/MS/MS using AB Sciex Q-StarElite MS instrument coupled with Agilent 1200 HPLC. See Table 11 andFIG. 18.

TABLE 11 Anti-GP73 MAb Epitope Peptide 1A-3187ELKKNEFQGELEKQREQLDKIQSSHNFQLESVNK (SEQ ID NO: 104) 1B-4971VSQENPEMEGPERDQLVIPDGQEEEQEAAGEGR (SEQ ID NO: 101) 1B-3246 EQVVEDRPVGGR(SEQ ID NO: 102) 1B-3440 LRGEDDYNMDENEAESETDK (SEQ ID NO: 103) 1A-4246Inconclusive 1B-4863 Inconclusive

Example 11 GP73 mAb Binding Kinetics

The affinities/kinetics of anti-human GP73 monoclonal antibodies1A-3187, 1A-4246, 1B-3246, 1B-3440, 1B-4863, and 1B-4971 for HEKexpressed recombinant GP73-hFc (Abbott, Ill., USA) were determined usinga Biacore 2000 instrument (GE Healthcare, Piscataway, N.J.). First, anapproximately 13,000-15,000 RU rabbit anti-mouse IgG Capture Biosensorwas created by amine-coupling rabbit anti-mouse IgG antibody (GEHealthcare) to a CM5 biosensor chip (GE Healthcare) viaEDC/NHS/ethanolamine chemistry provided in an Amine Coupling Kit (GEHealthcare) after pre-treating the biosensor with duplicate injectionsof 100 mM HCl, 50 mM NaOH, and 0.1% SDS. Purified GP73 antibody andantigen (HEK GP73-hFc) were diluted into a running buffer (hereinafter“running buffer”) composed of HBS-EP buffer (GE Healthcare) supplementedwith 0.1% BSA and 0.1% CM-Dextran. Each GP73 antibody was diluted to 1μg/mL. The GP73-hFc antigen was diluted to concentrations of 0.412 to100 nM using a 3-fold dilution series assuming an oligomeric molecularweight of 490 kDa (Abbott Laboratories).

The GP73-hFc antigen program was as follows. After equilibrating therabbit anti-mouse IgG Capture Biosensor for 3 min at 5 μL/minute withrunning buffer, GP73 antibody (7-14 μL) was injected over individualflow cells with one flow cell being left blank as a reference flow cell.The flow cells were washed with running buffer for 3 min at a flow rateof 60 μL/min. 150 μL of HEK293 expressed GP73-hFc antigen at a randomconcentration was injected across the biosensor. The biosensor wassubsequently re-equilibrated with running buffer for 5 min at a flowrate of 60 μL/minute. All biosensor surfaces were regenerated with one30 μL injection of 10 mM glycine, pH 1.7 (GE Healthcare), at a flow rateof 10 μL/minute. All concentrations of HEK293 expressed antigen weretested in duplicate. The binding kinetics (association and dissociation)were monitored via sensorgrams during antigen injection and runningbuffer re-equilibration. The sensorgrams were double-referenced and fitto a 1:1 binding model using Scrubber 2.0 software (BioLogic SoftwarePty Ltd., Australia) to determine association and dissociation rates, aswell as overall K_(D). The results are shown in Table 12. Standarderrors of determined values are reported in parentheses with respect tothe smallest number place value.

TABLE 12 GP73 monoclonal HEK Expressed GP73-hFc antibody k_(on) (M⁻¹s⁻¹)k_(off) (s⁻¹) K_(D) (M) 1A-3187 1.228(5) × 10⁵  2.5(1) × 10⁻⁴ 2.00(8) ×10⁻⁹  1A-4246  3.29(1) × 10⁵ 1.26(2) × 10⁻³ 3.83(5) × 10⁻⁹  1B-3246 3.18(1) × 10⁶  2.5(2) × 10⁻⁴  7.9(5) × 10⁻¹¹ 1B-3440 1.984(6) × 10⁶ 1.8(1) × 10⁻⁴  9.1(5) × 10⁻¹¹ 1B-4863 2.058(6) × 10⁶  2.5(1) × 10⁻⁴1.20(6) × 10⁻¹⁰ 1B-4971  3.90(2) × 10⁶   1(2) × 10⁻⁵   2(5) × 10⁻¹²

Example 12 ARCHITECT® GP73 Data

The ARCHITECT® sandwich immunoassay principle is shown in FIG. 19.Briefly, an antibody coated on a microparticle captures the analyte ofinterest, then a second antibody conjugated to acridinium binds to asecond epitope on the analyte, then a separation of the particles fromthe label and subsequent read is performed to determine the relativelight units (RLU) from the chemiluminescence reaction.

Monoclonal antibody pairs, such as 1A-4246 as a capture monoclonalantibody and 1B-4863 as a detection monoclonal antibody, were tested byELISA to mimic the same epitopes of GP73 that bind to the DrexelUniversity mouse monoclonal antibody 14H4-23 used as a capture antibodyand the rabbit polyclonal antibody used as a detection antibody. Theinitial ARCHITECT® experiment compared the Drexel capture antibody14H4-23 to the capture antibody 1A-4246. Each capture antibody waspaired with detection antibody 1-B4863, which was diluted to 0.5 nM. Theincorporation ratio of acridinium to antibody for 1B-4863 was 3.9. Asshown in FIG. 20, the capture antibody 1A-4246 outperformed the Drexelcapture antibody 14H4-23 by approximately three-fold based on thestandard curve using recombinant GP73-hFc fusion protein produced inHEK293 transient transfection with a range of 0-14.4 ng/mL See Table 13.

TABLE 13 ARCHITECT ® GP73 Sample ID ng/mL Mean Std. Dev. % CV CountRatio to CAL A Span to CAL A Data Replicates uP 14H4-23 coat 0.1 mg/mL,conj 1B4863 (IR 3.9) 0.5 nM cal A (rlu) 0 291.3 25.1 8.6 3 281 320 273cal B (rlu) 0.056 580.7 53.2 9.2 3 2.0 289.3 530 576 636 cal C (rlu)0.113 808.7 34.1 4.2 3 2.8 517.3 790 788 848 cal D (rlu) 0.225 1436.7104.5 7.3 3 4.9 1145.3 1453 1532 1325 cal E (rlu) 0.45 2493.0 115.6 4.63 8.6 2201.7 2521 2366 2592 cal F (rlu) 0.9 4522.0 235.3 5.2 3 15.54230.7 4254 4617 4695 cal G (rlu) 1.8 8555.7 259.4 3.0 3 29.4 8264.38507 8324 8836 cal H (rlu) 3.6 16995.3 376.4 2.2 3 58.3 16704.0 1740316922 16661 cal I (rlu) 7.2 33256.3 1752.1 5.3 3 114.2 32965.0 3191632614 35239 cal J (rlu) 14.4 63405.0 1124.1 1.8 3 217.6 63113.7 6259364688 62934 uP 1A4246 coat 0.1 mg/mL, conj 1B4863 (IR 3.9) 0.5 nM Cal A0 322 16.3 5.1 3 333 310 Cal B 0.056 1032 84.0 8.1 3 3.2 710.2 1033 1116947 Cal C 0.113 1855 166.8 9.0 3 5.8 1533.2 1823 1706 2035 Cal D 0.2253376 247.6 7.3 3 10.5 3054.2 3217 3661 3249 Cal E 0.45 6546 629.7 9.6 320.4 6224.5 6825 5825 6988 Cal F 0.9 12558 656.9 5.2 3 39.1 12236.211921 13233 12519 Cal G 1.8 24117 926.2 3.8 3 75.0 23795.2 23329 2388425137 Cal H 3.6 46080 777.4 1.7 3 143.3 45758.8 45635 46978 45628 Cal I7.2 89539 4212.5 4.7 3 278.5 89217.5 91530 84700 92387 Cal J 14.4 1737013197.5 1.8 3 540.3 173379.5 176903 173692 170508

In the follow up ARCHITECT® experiment, 1A-4246 was used as the captureantibody at a concentration of 0.2 mg/mL with 1B-4863 used as thedetection antibody at a concentration of 0.5 nM or 2 nM. Theincorporation ratio of acridinium to antibody for 1B-4863 was 6.18. Thehigher conjugate concentration of 2 nM produces a greater signal asexpected (see FIG. 21), but there was higher background in Ca1 A (seeTable 14). This GP73 ARCHITECT® data shows a very linear response to therecombinant GP73-hFc antigen for both monoclonal antibody pairs.

TABLE 14 ARCHITECT ® GP73 Sample ID: ng/mL Mean Std. Dev. % CV CountRatio to CAL A Span to CAL A Data Replicates 1A4246 uP coated 0.2 mg/mL,conj 1B4863 0.5 nM IR 6.18 Cal A 0 546 57.3 10.5 3 606 539 492 Cal B0.056 2599 147.5 5.7 3 4.8 2053.3 2757 2465 2575 Cal C 0.113 4630 192.64.2 3 8.5 4084.0 4638 4818 4433 Cal D 0.225 8221 148.3 1.8 3 15.1 7675.78063 8357 8244 Cal E 0.45 16146 697.1 4.3 3 29.6 15600.7 16849 1613515455 Cal F 0.9 30829 247.0 0.8 3 56.5 30283.3 31001 30546 30940 Cal G1.8 60219 1095.7 1.8 3 110.4 59673.0 59211 61385 60060 Cal H 3.6 1167372887.4 2.5 3 213.9 116191.3 117598 113517 119096 Cal I 7.2 213371 6890.73.2 3 391.0 212825.7 205710 215342 219062 Cal J 14.4 420567 9171.0 2.2 3770.7 420021.7 427394 410143 424165 1A4246 uP coated 0.2 mg/mL, conj1B4863 2 nM IR 6.18 Cal A 0 2489 593.3 23.8 3 1878 2525 3063 Cal B 0.0565569 328.8 5.9 3 2.2 3080.7 5334 5945 5429 Cal C 0.113 9942 656.4 6.6 34.0 7453.7 10155 9206 10466 Cal D 0.225 16465 921.9 5.6 3 6.6 13976.715896 15971 17529 Cal E 0.45 30421 972.2 3.2 3 12.2 27932.7 29460 3140430400 Cal F 0.9 58437 603.1 1.0 3 23.5 55948.3 58869 58694 57748 Cal G1.8 113539 2427.9 2.1 3 45.6 111050.3 116218 112915 111484 Cal H 3.6209923 1191.0 0.6 3 84.4 207434.7 209531 211261 208978 Cal I 7.2 4049765626.5 1.4 3 162.7 402487.0 409800 398795 406332 Cal J 14.4 74249719168.7 2.6 3 298.4 740008.3 747483 758680 721328

It is understood that the foregoing detailed description andaccompanying examples are merely illustrative and are not to be taken aslimitations upon the scope of the invention, which is defined solely bythe appended claims and their equivalents.

Various changes and modifications to the disclosed embodiments will beapparent to those skilled in the art. Such changes and modifications,including without limitation those relating to the chemical structures,substituents, derivatives, intermediates, syntheses, compositions,formulations, or methods of use of the invention, may be made withoutdeparting from the spirit and scope thereof.

1. An isolated antibody or antibody fragment thereof capable of bindingan epitope within the amino acid sequence of selected from the groupconsisting of: i) (SEQ ID NO: 101) VSQENPEMEGPERDQLVIPDGQEEEQEAAGEGR(hGP73 307-339); ii) (SEQ ID NO: 102) EQVVEDRPVGGR (hGP73 276-287); iii)(SEQ ID NO: 103 LRGEDDYNMDENEAESETDK (hGP73 344-363); and iv) (SEQ IDNO: 104) ELKKNEFQGELEKQREQLDKIQSSHNFQLESVNK (hGP73 63-96)

2-16. (canceled)
 17. An isolated antibody or antibody fragment thereofwhich immunospecifically binds to Golgi Protein 73 (“GP73”), wherein thebinding of the antibody or antibody fragment to GP73 is: i) sensitive tofucosylation of GP73; or ii) insensitive to fucosylation of GP73. 18-21.(canceled) 22-24. (canceled)
 25. An isolated antibody or antibodyfragment thereof which binds to Golgi Protein 73 (“GP73”), wherein theantibody comprises a domain or region selected from the group consistingof: (a) a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:1, (b) a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:5, (c) a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:9, (d) a variablelight domain region comprising the amino acid sequence of SEQ ID NO:13,(e) a variable heavy domain region comprising the amino acid sequence ofSEQ ID NO:17, (f) a variable light domain region comprising the aminoacid sequence of SEQ ID NO:21, (g) a variable heavy domain regioncomprising the amino acid sequence of SEQ ID NO:25, (h) a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:29, (i) avariable heavy domain region comprising the amino acid sequence of SEQID NO:33, (j) a variable light domain region comprising the amino acidsequence of SEQ ID NO:37; (k) a variable heavy domain region comprisingthe amino acid sequence of SEQ ID NO:41; (l) a variable light domainregion comprising the amino acid sequence of SEQ ID NO:45; (m) variableheavy domain comprising the amino acid sequence of SEQ ID NO:1 and avariable light domain region comprising the amino acid sequence of SEQID NO:5, (n) a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:9 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:13, (o) a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:17 and a variable light domainregion comprising the amino acid sequence of SEQ ID NO:21, (p) avariable heavy domain comprising the amino acid sequence of SEQ ID NO:25and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:29, (q) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:33 and a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:37, (r) a variably heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:45, (s) avariable heavy chain comprising a complementarity determining region(CDR)1 comprising the amino acid sequence of SEQ ID NO:2, a CDR2comprising the amino acid sequence of SEQ ID NO:3, and a CDR3 comprisingthe amino acid sequence of SEQ ID NO:4, (t) a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:6, aCDR2 comprising the amino acid sequence of SEQ ID NO:7, and a CDR3comprising the amino acid sequence of SEQ ID NO:8, (u) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and aCDR3 comprising the amino acid sequence of SEQ ID NO:12, (v) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:14, a CDR2 comprising the amino acid sequence of SEQ ID NO:15, anda CDR3 comprising the amino acid sequence of SEQ ID NO:16, (w) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequence ofSEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20, (x) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24, (y) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequenceof SEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28, (z) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32, (aa) a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:34, a CDR2 comprising the amino acidsequence of SEQ ID NO:35, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:36, (bb) a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:38, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:39, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:40, (cc) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and CDR3 comprisingthe amino acid sequence of SEQ ID NO:44; (dd) a variable light chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:46, aCDR2 comprising the amino acid sequence of SEQ ID NO:47, and a CDR3comprising the amino acid sequence of SEQ ID NO:48; (ee) a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4 and a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8, (ff) a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, anda CDR3 comprising the amino acid sequence of SEQ ID NO:12, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequence ofSEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16, (gg) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:18, a CDR2 comprising the amino acidsequence of SEQ ID NO:19, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:20, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:22, a CDR2 comprising the aminoacid sequence of SEQ ID NO:23, and a CDR3 comprising the amino acidsequence of SEQ ID NO:24, (hh) a variable heavy chain comprising a CDR 1comprising the amino acid sequence of SEQ ID NO:26, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:27, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:28, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:30, a CDR2comprising the amino acid sequence of SEQ ID NO:31, and a CDR3comprising the amino acid sequence of SEQ ID NO:32, (ii) a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, anda CDR3 comprising the amino acid sequence of SEQ ID NO:36, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequence ofSEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40, and (jj) a variable heavy domain chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:42, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:43, and a CDR comprising the aminoacid sequence of SEQ ID NO:44, and a variable light domain chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:46, aCDR2 comprising the amino acid sequence of SEQ ID NO:47, and a CDR3comprising the amino acid sequence of SEQ ID NO:48. 26-28. (canceled)29. The isolated antibody or antibody fragment of claim 25, wherein theantibody or antibody fragment comprises: i) a variable heavy regioncomprising a sequence selected from the group consisting of SEQ ID NO:1,SEQ ID NO:9, SEQ ID NO:17, SEQ ID NO:25, SEQ ID NO:33, and SEQ ID NO:41;ii. a variable light region comprising a sequence selected from thegroup consisting of SEQ ID NO:5, SEQ ID NO:13, SEQ ID NO:21, SEQ IDNO:29, SEQ ID NO:37, and SEQ ID NO:45; iii a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:1 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:5; iv. avariable heavy domain comprising the amino acid sequence of SEQ ID NO:9and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:13; v. a variable heavy domain comprising the amino acidsequence of SEQ ID NO:17 and a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:21; vi. a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:29 vii. avariable heavy domain comprising the amino acid sequence of SEQ ID NO:33and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:37; or viii. a variably heavy domain comprising the amino acidsequence of SEQ ID NO:41 and a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:45. 30-36. (canceled)
 37. Theisolated antibody or antibody fragment of claim 25, wherein the antibodyor antibody fragment comprises a variable heavy domain that comprisescomplementarity-determining region (CDR) residues: (a) SEQ ID NO:2, SEQID NO:3, and SEQ ID NO:4; (b) SEQ ID NO:10, SEQ ID NO:11, and SEQ IDNO:12; (c) SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:20; (d) SEQ IDNO:26, SEQ ID NO:27, and SEQ ID NO:28; (e) SEQ ID NO:34, SEQ ID NO:35,and SEQ ID NO:36; or (f) SEQ ID NO:42, SEQ ID NO:43, and SEQ ID NO:44.38. The isolated antibody or antibody fragment of claim 25, wherein theantibody or antibody fragment comprises a variable light domain thatcomprises complementarity-determining region (CDR) residues: (a) SEQ IDNO:6, SEQ ID NO:7, and SEQ ID NO:8; (a) SEQ ID NO:14, SEQ ID NO:15, andSEQ ID NO:16; (b) SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24; (c) SEQID NO:30, SEQ ID NO:31, and SEQ ID NO:32; (d) SEQ ID NO:38, SEQ IDNO:39, and SEQ ID NO:40; or (e) SEQ ID NO:46, SEQ ID NO:47, and SEQ IDNO:48.
 39. The isolated antibody or antibody fragment of claim 25,wherein the antibody or antibody fragment comprises: i) a variable heavydomain that comprises complementarity-determining region (CDR) residuesSEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4, and a variable light domainthat comprises complementarity-determining region (CDR) residues SEQ IDNO:6, SEQ ID NO:7, and SEQ ID NO:8; ii) a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:10, SEQ ID NO:11, and SEQ ID NO:12, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:14, SEQ ID NO:15, and SEQ ID NO:16; iii) a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:18, SEQ ID NO:19, and SEQ ID NO:20, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:22, SEQ ID NO:23, and SEQ ID NO:24; iv) a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:26, SEQ ID NO:27, and SEQ ID NO:28, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:30, SEQ ID NO:31, and SEQ ID NO:32; v) a variable heavy domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:34, SEQ ID NO:35, and SEQ ID NO:36, and a variable light domain thatcomprises complementarity-determining region (CDR) residues SEQ IDNO:38, SEQ ID NO:39, and SEQ ID NO:40; or vi) comprises a variable heavydomain that comprises complementarity-determining region (CDR) residuesSEQ ID NO:42, SEQ ID NO:43, and SEQ ID NO:44, and a variable lightdomain that comprises complementarity-determining region (CDR) residuesSEQ ID NO:46, SEQ ID NO:47, and SEQ ID NO:48. 40.-47. (canceled)
 48. Theisolated antibody or antibody fragment of claim 25, wherein the antibodyor antibody fragment binds to the GP73 epitope within the amino acidsequence of: i) (SEQ ID NO: 101) VSQENPEMEGPERDQLVIPDGQEEEQEAAGEGR(hGP73 307-339); ii) (SEQ ID NO: 102 EQVVEDRPVGGR (hGP73 276-287); iii)(SEQ ID NO: 103) LRGEDDYNMDENEAESETDK (hGP73 344-363) iv) (SEQ ID NO:104) ELKKNEFQGELEKQREQLDKIQSSHNFQLESVNK (hGP73 63-96)..


49. The isolated antibody or antibody fragment of claim 48, wherein theantibody or antibody fragment comprises: (a) a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:9; (b) a variablelight domain region comprising the amino acid sequence of SEQ ID NO:13;(c) a variable heavy domain comprising the amino acid sequence of SEQ IDNO:9 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:13; (d) a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:10, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:12; (e) a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:14, a CDR2comprising the amino acid sequence of SEQ ID NO:15, and a CDR3comprising the amino acid sequence of SEQ ID NO:16; or (f) a variableheavy chain comprising a CDR 1 comprising the amino acid sequence of SEQID NO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, anda CDR3 comprising the amino acid sequence of SEQ ID NO:12, and avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequence ofSEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16.
 50. (canceled)
 51. The isolated antibody or antibody fragment ofclaim 48, wherein the antibody or antibody fragment comprises: (a) avariable heavy domain region comprising the amino acid sequence of SEQID NO:17; (b) a variable light domain region comprising the amino acidsequence of SEQ ID NO:21; (c) a variable heavy domain comprising theamino acid sequence of SEQ ID NO:17 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:21; (d) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:18, a CDR2 comprising the amino acid sequence of SEQ ID NO:19, and aCDR3 comprising the amino acid sequence of SEQ ID NO:20; (e) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, anda CDR3 comprising the amino acid sequence of SEQ ID NO:24; or (f) avariable heavy chain comprising a CDR 1 comprising the amino acidsequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequence ofSEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24.
 52. (canceled)
 53. The isolated antibody or antibody fragment ofclaim 48, wherein the antibody or antibody fragment comprises: (a) avariable heavy domain region comprising the amino acid sequence of SEQID NO:1; (b) a variable light domain region comprising the amino acidsequence of SEQ ID NO:5; (c) a variable heavy domain comprising theamino acid sequence of SEQ ID NO:1 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:5; (d) a variable heavychain comprising a complementarity determining region (CDR)1 comprisingthe amino acid sequence of SEQ ID NO:2, a CDR2 comprising the amino acidsequence of SEQ ID NO:3, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:4; (e) a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:6, a CDR2 comprising the amino acidsequence of SEQ ID NO:7, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:8; or (f) a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:2, a CDR2 comprising theamino acid sequence of SEQ ID NO:3, and a CDR3 comprising the amino acidsequence of SEQ ID NO:4 and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:6, a CDR2 comprising theamino acid sequence of SEQ ID NO:7, and a CDR3 comprising the amino acidsequence of SEQ ID NO:8.
 54. (canceled)
 55. The isolated antibody orantibody fragment of claim 48, wherein the antibody or antibody fragmentcomprises: (a) a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:33; (b) a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:37; (c) a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:33 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:37; (d) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:34, a CDR2 comprising the amino acid sequence ofSEQ ID NO:35, and a CDR3 comprising the amino acid sequence of SEQ IDNO:36; (e) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequenceof SEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40; or (f) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:34, a CDR2 comprising the amino acidsequence of SEQ ID NO:35, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:36, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:38, a CDR2 comprising the aminoacid sequence of SEQ ID NO:39, and a CDR3 comprising the amino acidsequence of SEQ ID NO:40.
 56. The isolated antibody or antibody fragmentof claim 25, wherein the binding of the antibody or antibody fragment toGP73 is: i) sensitive to the presence or absence of a fucose sugarmoiety on GP73; or ii) insensitive to the presence or absence of afucose sugar moiety on GP73.
 57. The isolated antibody or antibodyfragment of claim 56, wherein the antibody or antibody fragmentcomprises: (a) a variable heavy domain region comprising the amino acidsequence of SEQ ID NO:25 or SEQ ID NO:33; (b) a variable light domainregion comprising the amino acid sequence of SEQ ID NO:29 or SEQ IDNO:37; (c) a variable heavy domain comprising the amino acid sequence ofSEQ ID NO:25 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:29; (d) a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:33 and a variable light domainregion comprising the amino acid sequence of SEQ ID NO:37; (e) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence ofSEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; (f) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:34, a CDR2 comprising the amino acid sequenceof SEQ ID NO:35, and a CDR3 comprising the amino acid sequence of SEQ IDNO:36; (g) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; (h) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequenceof SEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40; (i) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:26, a CDR2 comprising the amino acidsequence of SEQ ID NO:27, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:28, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:30, a CDR2 comprising the aminoacid sequence of SEQ ID NO:31, and a CDR3 comprising the amino acidsequence of SEQ ID NO:32; or (j) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:38, a CDR2comprising the amino acid sequence of SEQ ID NO:39, and a CDR3comprising the amino acid sequence of SEQ ID NO:40.
 58. (canceled) 59.The isolated antibody or antibody fragment of claim 56, wherein theantibody or antibody fragment comprises: (a) a variable heavy domainregion comprising the amino acid sequence of SEQ ID NO:1, SEQ ID NO:9,SEQ ID NO:17, or SEQ ID NO:41; (b) a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:13, SEQ IDNO:21, or SEQ ID NO:45; (c) a variable heavy domain comprising the aminoacid sequence of SEQ ID NO:1 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:5; (d) a variable heavydomain comprising the amino acid sequence of SEQ ID NO:9 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:13;(e) a variable heavy domain comprising the amino acid sequence of SEQ IDNO:17 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:21; (f) a variable heavy domain comprising theamino acid sequence of SEQ ID NO:41 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:45; (g) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:2, a CDR2 comprising the amino acid sequence of SEQ ID NO:3, and aCDR3 comprising the amino acid sequence of SEQ ID NO:4; (h) a variableheavy chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:10, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, anda CDR3 comprising the amino acid sequence of SEQ ID NO:12; (i) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequence ofSEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20; (j) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44; (k) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; (l) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequenceof SEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; (m) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; (n) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; (o) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:2, a CDR2 comprising the amino acidsequence of SEQ ID NO:3, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:4, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:6, a CDR2 comprising the amino acidsequence of SEQ ID NO:7, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:8; (p) a variable heavy chain comprising a CDR 1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12, and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16; (q) a variable heavy chain comprising aCDR 1 comprising the amino acid sequence of SEQ ID NO:18, a CDR2comprising the amino acid sequence of SEQ ID NO:19, and a CDR3comprising the amino acid sequence of SEQ ID NO:20, and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, and aCDR3 comprising the amino acid sequence of SEQ ID NO:24; or (r) avariable heavy chain comprising a CDR 1 comprising the amino acidsequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequence ofSEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48.
 60. An isolated antibody that specifically binds GP-73 and has aCDR-H1 having the formula: X₅-X₆-X₇-X₈-X₉ (SEQ ID NO:108) a CDR-H2having the formula:X₁₀-I-X₁₁-X₁₂-X₁₃-X₁₄-X₁₅-X₁₆X₁₇-X₁₈-X₁₉-X₂₀-X₂₁-X₂₂-X₂₃-X₂₄-X₂₅-X₂₆-X₂₇(SEQ ID NO:109), a CDR-H3 having the formula:X₂₈-X₂₉-X₃₀-X₃₁-X₃₂-X₃₃-X₃₄-X₃₅-X₃₆-X₃₇-X₃₈-X₃₉-Y (SEQ ID NO:110), aCDR-L1 having the formula:X₄₀-X₄₁-S-X₄₂-X₄₃-X₄₄-X₄₅-X₄₆-X₄₇-X₄₈-X₄₉-X₅₀-X₅₁-X₅₂-X₅₃-X₅₄ (SEQ IDNO:111), a CDR-L2 having the formula: X₅₅-X₅₆-S-X₅₇-X₅₈-X₅₉-X₆₀ (SEQ IDNO:112) and a CDR-L3 having the formula: X₆₁-Q-X₆₂-X₆₃-X₆₄-X₆₅-P-X₆₆-T(SEQ ID NO:113), wherein: X₅ is S, N or T; X₆ is Y or N; X₇ is W, V, G,A or T; X₈ is I, V or M; X₉ is E, H, S or N; X₁₀ is E, Y, V, T or R; X₁₁is L, W, S or R; X₁₂ is P, S, R or T; X₁₃ is absent or is K; X₁₄ isabsent or is R; X₁₅ is G, T or Y; X₁₆ is S, G or N; X₁₇ is G, D, S, T orY; X₁₈ is N, S, Y, G or T; X₁₉ is T or I; X₂₀ is N, K, Y or F; X₂₁ is Yor F; X₂₂ is N, P or A; X₂₃ is E, S or D; X₂₄ is K, A or S; X₂₅ is F, Lor V; X₂₆ is K or M; X₂₇ is G, S or D; X₂₈ is G, Q, D, E or N; X₂₉ is R,Q, P, Y, W or G; X₃₀ is G, L, F, D or T; X₃₁ is S, T, S or G; X₃₂ is Y,D, G, E or T; X₃₃ is R, Y, D, L or F; X₃₄ is Y, F, T or H; X₃₅ is H orY; X₃₆ is W, D or Y; X₃₇ is F, Y or A; X₃₈ is absent or is F or M; X₃₉is A or D; X₄₀ is K, T, R or S; X₄₁ is A or S; X₄₂ is Q, S or K; X₄₃ isS or G; X₄₄ is V or L; X₄₅ is D or L; X₄₆ is Y, D or H; X₄₇ is absent orS; X₄₈ is D, V, N or I; X₄₉ is G, V or S; X₅₀ is D, K, S or I; X₅₁ is S,T, I or N; X₅₂ is Y or D; X₅₃ is M, L or V; X₅₄ is N, I, S, H or Y; X₅₅is A, L, S, Q or R; X₅₆ is A, V, T or M; X₅₇ is N, K, Y or S; X₅₈ is Lor R; X₅₉ is E, D, Y or A; X₆₀ is S or I; X₆₁ is Q, W, H or A; X₆₂ is S,G, H, Y or N; X₆₃ is N, T, F, H or L; X₆₄ is E, H, T, R or S; X₆₅ is D,F, T, S, L or I; and X₆₆ is Y or L.
 61. An isolated antibody or antibodyfragment thereof which immunospecifically binds to Golgi Protein 73(“GP73”), wherein said antibody has: i) an equilibrium dissociationconstant (K_(D)) of between about 4.0×10⁻⁹ to about 1.8×10⁻¹²; ii) has adissociation rate (k_(off)) of between about 1.5×10⁻³ to about 8.0×10⁻⁶;iii) an association rate (k_(on)) of between about 1.0×10⁵ to about4.1×10⁶; or iv) an association rate (k_(on)) of between about 1.0×10⁵ toabout 4.1×10⁶. 62-69. (canceled)
 70. A pharmaceutical compositioncomprising the antibody, antibody fragment, mixture or derivativethereof of claim
 25. 71. A method for determining GP73 concentration ina test sample, the method comprising: (a) contacting the test samplewith at least one capture antibody, wherein the capture antibody bindsto an epitope on GP73 or a fragment of GP73 to form a captureantibody-GP73 antigen complex; (b) contacting the capture antibody-GP73antigen complex with at least one detection antibody comprising adetectable label, wherein the detection antibody binds to an epitope onGP73 that is not bound by the capture antibody and forms a captureantibody-GP73 antigen-detection antibody complex; and (c) determiningthe GP73 concentration in the test sample based on the signal generatedby the detectable label in the capture antibody-GP73 antigen-detectionantibody complex formed in (b); wherein the at least one captureantibody comprises the isolated antibody or antibody fragment of 25 andthe at least one detection antibody comprises the isolated antibody orfragment antibody of 25, and wherein the least one capture antibody isdifferent from the at least one detection antibody.
 72. The method ofclaim 71, further comprising comparing the signal generated by thedetectable label as a direct or indirect indication of the GP73concentration in the test sample to a signal generated as a direct orindirect indication of the GP73 concentration in a control orcalibrator.
 73. The method of claim 72, wherein the GP73 concentrationin the test sample is used to determine or assess whether a subject hasor is at risk of developing liver disease.
 74. The method of claim 73,wherein an increased GP73 concentration as compared to the GP73concentration in a control or calibrator indicates that the subject hasliver disease.
 75. The method of claim 74, wherein the liver disease isliver cirrhosis or liver cancer.
 76. A method for determining GP73concentration in a test sample, the method comprising: (a) contactingthe test sample with at least one capture antibody, wherein the captureantibody binds to an epitope on GP73 or a fragment of GP73 to form acapture antibody-GP73 antigen complex; (b) contacting the captureantibody-GP73 antigen complex with at least one detection antibodycomprising a detectable label, wherein the detection antibody binds toan epitope on GP73 that is not bound by the capture antibody and forms acapture antibody-GP73 antigen-detection antibody complex; and (c)determining the GP73 concentration in the test sample based on thesignal generated by the detectable label in the capture antibody-GP73antigen-detection antibody complex formed in (b); wherein the at leastone capture antibody comprises: (i) a variable heavy domain comprisingthe amino acid sequence of SEQ ID NO:41; (ii) a variable light domaincomprising the amino acid sequence of SEQ ID NO:45; (iii) a variableheavy domain comprising the amino acid sequence of SEQ ID NO:41 and avariable light domain comprising the amino acid sequence of SEQ IDNO:45; (iv) a variable heavy chain comprising a complementaritydetermining region (CDR)1 comprising the amino acid sequence of SEQ IDNO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, and aCDR3 comprising the amino acid sequence of SEQ ID NO:44; (v) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, anda CDR3 comprising the amino acid sequence of SEQ ID NO:48; or (vi) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequence ofSEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; wherein the at least one detection antibody comprises: (vii) avariable heavy domain comprising the amino acid sequence of SEQ IDNO:25; (viii) a variable light domain comprising the amino acid sequenceof SEQ ID NO:29; (ix) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:25 and a variable light domain comprising theamino acid sequence of SEQ ID NO:29; (x) a variable heavy chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:26, aCDR2 comprising the amino acid sequence of SEQ ID NO:27, and a CDR3comprising the amino acid sequence of SEQ ID NO:28; (xi) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:30, a CDR2 comprising the amino acid sequence of SEQ ID NO:31, anda CDR3 comprising the amino acid sequence of SEQ ID NO:32; or (xii) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence ofSEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32.
 77. A method for determining fucosylated GP73 concentration in atest sample, the method comprising; (a) contacting the test sample withat least one capture binding protein, wherein the capture bindingprotein binds to a region of GP73 or a fragment of GP73 to form acapture binding protein-GP73 complex; (b) contacting the capture bindingprotein-GP73 complex with at least one detection binding proteincomprising a detectable label, wherein the detection binding proteinbinds to a region of GP73 that is not bound by the capture bindingprotein and forms a capture binding protein-GP73-detection bindingprotein complex; and (c) determining the GP73 concentration in the testsample based on the signal generated by the detectable label in thecapture binding protein-GP73-detection binding protein complex formed in(b); wherein the at least one capture binding protein comprises aprotein, antibody or antibody fragment whose binding to GP73 issensitive to the presence or absence of a fucose sugar moiety on GP73and the at least one detection binding protein comprises a protein,antibody or antibody fragment whose binding to GP73 is insensitive tothe presence or absence of a fucose sugar moiety on GP73.
 78. A methodfor determining fucosylated GP73 concentration in a test sample, themethod comprising: (a) contacting the test sample with at least onecapture binding protein, wherein the capture binding protein binds to aregion of GP73 or a fragment of GP73 to form a capture bindingprotein-GP73 complex; (b) contacting the capture binding protein-GP73complex with at least one detection binding protein comprising adetectable label, wherein the detection binding protein binds to aregion of GP73 that is not bound by the capture binding protein andforms a capture binding protein-GP73-detection binding protein complex;and (c) determining the GP73 concentration in the test sample based onthe signal generated by the detectable label in the capture bindingprotein-GP73-detection binding protein complex formed in (b); whereinthe at least one capture binding protein comprises a protein, antibodyor antibody fragment whose binding to GP73 is insensitive to thepresence or absence of a fucose sugar moiety on GP73 and the at leastone detection binding protein comprises a protein, antibody or antibodyfragment whose binding to GP73 is sensitive the presence or absence of afucose sugar moiety on GP73.
 79. The method of claim 77, wherein theprotein, antibody or antibody fragment whose binding to GP73 issensitive to the presence or absence of a fucose sugar moiety on GP73comprises: (a) Aleuria aurantia lectin (AAL) or a fragment thereof; (b)a variable heavy domain region comprising the amino acid sequence of SEQID NO:25 or SEQ ID NO:33; (c) a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:29 or SEQ ID NO:37; (d) a variableheavy domain comprising the amino acid sequence of SEQ ID NO:25 and avariable light domain region comprising the amino acid sequence of SEQID NO:29; (e) a variable heavy domain comprising the amino acid sequenceof SEQ ID NO:33 and a variable light domain region comprising the aminoacid sequence of SEQ ID NO:37; (f) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:26, a CDR2comprising the amino acid sequence of SEQ ID NO:27, and a CDR3comprising the amino acid sequence of SEQ ID NO:28; (g) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:34, a CDR2 comprising the amino acid sequence of SEQ ID NO:35, and aCDR3 comprising the amino acid sequence of SEQ ID NO:36; (h) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:30, a CDR2 comprising the amino acid sequence of SEQ ID NO:31, anda CDR3 comprising the amino acid sequence of SEQ ID NO:32; (i) avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequence ofSEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40; (j) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:26, a CDR2 comprising the amino acidsequence of SEQ ID NO:27, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:28, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:30, a CDR2 comprising the aminoacid sequence of SEQ ID NO:31, and a CDR3 comprising the amino acidsequence of SEQ ID NO:32; or (k) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:38, a CDR2comprising the amino acid sequence of SEQ ID NO:39, and a CDR3comprising the amino acid sequence of SEQ ID NO:40.
 80. The method ofclaim, wherein the protein, antibody or antibody fragment whose bindingto GP73 is insensitive to the presence or absence of a fucose sugarmoiety on GP73 comprises: (a) a variable heavy domain region comprisingthe amino acid sequence of SEQ ID NO:1, SEQ ID NO:9, SEQ ID NO:17, orSEQ ID NO:41; (b) a variable light domain region comprising the aminoacid sequence of SEQ ID NO:5, SEQ ID NO:13, SEQ ID NO:21, or SEQ IDNO:45; (c) a variable heavy domain comprising the amino acid sequence ofSEQ ID NO:1 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:5; (d) a variable heavy domain comprising theamino acid sequence of SEQ ID NO:9 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:13; (e) a variable heavydomain comprising the amino acid sequence of SEQ ID NO:17 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:21;(f) a variable heavy domain comprising the amino acid sequence of SEQ IDNO:41 and a variable light domain region comprising the amino acidsequence of SEQ ID NO:45; (g) a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:2, a CDR2 comprising theamino acid sequence of SEQ ID NO:3, and a CDR3 comprising the amino acidsequence of SEQ ID NO:4; (h) a variable heavy chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:10, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:12; (i) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:18, a CDR2comprising the amino acid sequence of SEQ ID NO:19, and a CDR3comprising the amino acid sequence of SEQ ID NO:20; (j) a variable heavychain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, and aCDR3 comprising the amino acid sequence of SEQ ID NO:44; (k) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:6, a CDR2 comprising the amino acid sequence of SEQ ID NO:7, and aCDR3 comprising the amino acid sequence of SEQ ID NO:8; (l) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:14, a CDR2 comprising the amino acid sequence of SEQ ID NO:15, anda CDR3 comprising the amino acid sequence of SEQ ID NO:16; (m) avariable light chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequence ofSEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; (n) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; (o) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:2, a CDR2 comprising the amino acidsequence of SEQ ID NO:3, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:4, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:6, a CDR2 comprising the amino acidsequence of SEQ ID NO:7, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:8; (p) a variable heavy chain comprising a CDR 1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12, and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16; (q) a variable heavy chain comprising aCDR 1 comprising the amino acid sequence of SEQ ID NO:18, a CDR2comprising the amino acid sequence of SEQ ID NO:19, and a CDR3comprising the amino acid sequence of SEQ ID NO:20, and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, and aCDR3 comprising the amino acid sequence of SEQ ID NO:24; or (r) avariable heavy chain comprising a CDR 1 comprising the amino acidsequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequence ofSEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48.
 81. The method of claim 77, further comprising comparing thesignal generated by the detectable label as a direct or indirectindication of the GP73 concentration in the test sample to a signalgenerated as a direct or indirect indication of the GP73 concentrationin a control or calibrator.
 82. The method of claim 81, wherein the GP73concentration in the test sample is used to determine or assess whethera subject has or is at risk of developing liver disease.
 83. The methodof claim 82, wherein an increased GP73 concentration as compared to theGP73 concentration in a control or calibrator indicates that the subjecthas liver disease.
 84. The method of claim 83, wherein the liver diseaseis liver cirrhosis or liver cancer.
 85. A method of diagnosing andtreating liver disease in a subject, the method comprising: (a)obtaining a biological sample comprising blood from the subject; (b)determining the GP73 concentration in the biological sample from thesubject using the method of claim 81; (c) comparing the GP73concentration in the biological sample with the GP73 concentration in anormal control or calibrator; (d) diagnosing the subject as having liverdisease if the GP73 concentration in the biological sample is greaterthan the GP73 concentration in the normal control or calibrator; and (e)administering a liver disease treatment regimen to the subject diagnosedas having liver disease.
 86. (canceled)
 87. The method of claim 85,wherein the biological sample of a subject is blood plasma or bloodserum.
 88. The method of claim 85, wherein the liver disease is livercirrhosis or liver cancer.
 89. The method of claim 85, furthercomprising determining the level of at least one additional biomarker ofliver disease in the biological sample, and comparing the level of theat least one additional biomarker of liver disease to a referenceconcentration value for the at least one biomarker of liver disease. 90.The method of claim 89, wherein the additional biomarker of liverdisease is selected from the group consisting of PIVKA-II, AFP, AFP-L3,Fuc-HPX, Fc-Kin and F-AT.
 91. A method for determining if a subject isresponding to the administration of one or more pharmaceuticalcompositions, the method comprising: (a) measuring the GP73concentration in a sample from the subject using the method of claim 81;(b) comparing the GP73 concentration in the sample with the GP73concentration in a normal control or calibrator, wherein an altered GP73concentration indicates that the subject is not responding to theadministration of one or more pharmaceutical compositions; and (c)adjusting the treatment of the subject if the subject is not respondingto the administration of one or more pharmaceutical compositions.
 92. Amethod of diagnosing and treating liver disease in a subject, the methodcomprising: (a) contacting the test sample with at least one captureantibody, wherein the capture antibody binds to an epitope on GP73 or afragment of GP73 to form a capture antibody-GP73 antigen complex; (b)contacting the capture antibody-GP73 antigen complex with at least onedetection antibody comprising a detectable label, wherein the detectionantibody binds to an epitope on GP73 that is not bound by the captureantibody and forms a capture antibody-GP73 antigen-detection antibodycomplex; (c) determining the GP73 concentration in the test sample basedon the signal generated by the detectable label in the captureantibody-GP73 antigen-detection antibody complex formed in (b); (d)comparing the GP73 concentration in the sample with the GP73concentration in a normal control or calibrator; (e) diagnosing thesubject as having liver disease if the GP73 concentration in thebiological sample is greater than the GP73 concentration in the normalcontrol or calibrator; and (f) administering a liver disease treatmentregimen to the subject diagnosed as having liver disease; wherein the atleast one capture antibody comprises: (i) a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:41; (ii) a variablelight domain comprising the amino acid sequence of SEQ ID NO:45; (iii) avariable heavy domain comprising the amino acid sequence of SEQ ID NO:41and a variable light domain comprising the amino acid sequence of SEQ IDNO:45; (iv) a variable heavy chain comprising a complementaritydetermining region (CDR)1 comprising the amino acid sequence of SEQ IDNO:42, a CDR2 comprising the amino acid sequence of SEQ ID NO:43, and aCDR3 comprising the amino acid sequence of SEQ ID NO:44; (v) a variablelight chain comprising a CDR1 comprising the amino acid sequence of SEQID NO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, anda CDR3 comprising the amino acid sequence of SEQ ID NO:48; or (vi) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequence ofSEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; wherein the at least one detection antibody comprises: (i) avariable heavy domain comprising the amino acid sequence of SEQ IDNO:25; (ii) a variable light domain comprising the amino acid sequenceof SEQ ID NO:29; (iii) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:25 and a variable light domain comprising theamino acid sequence of SEQ ID NO:29; (iv) a variable heavy chaincomprising a CDR1 comprising the amino acid sequence of SEQ ID NO:26, aCDR2 comprising the amino acid sequence of SEQ ID NO:27, and a CDR3comprising the amino acid sequence of SEQ ID NO:28; (v) a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:30, a CDR2 comprising the amino acid sequence of SEQ ID NO:31, and aCDR3 comprising the amino acid sequence of SEQ ID NO:32; or (vi) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence ofSEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28 and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32.
 93. The method of claim 92, wherein the liver disease is livercirrhosis or liver cancer.
 94. A kit comprising the antibody, antibodyfragment, mixture or derivative thereof of claim
 25. 95. A kitcomprising an isolated nucleic acid encoding the antibody or antibodyfragment of claim
 25. 96. A kit for assaying a test sample for GP73,which kit comprises at least one capture antibody, wherein the captureantibody binds to an epitope on GP73 or a fragment of GP73, at least onedetection antibody, wherein the detection antibody binds to an epitopeon GP73 that is not bound by the capture antibody, and instructions forassaying the test sample for GP73, wherein the at least one captureantibody comprises the isolated antibody or antibody fragment of claim25 and the at least one detection antibody comprises the isolatedantibody or antibody fragment of claim 25, and wherein the least onecapture antibody is different from the at least one detection antibody.97. The kit of claim 96, further comprising a reference standardindicating a GP73 concentration in a control or calibrator.
 98. A kitfor assaying a test sample for GP73, which kit comprises at least onecapture antibody, wherein the capture antibody binds to an epitope onGP73 or a fragment of GP73, at least one detection antibody, wherein thedetection antibody binds to an epitope on GP73 that is not bound by thecapture antibody, and instructions for assaying the test sample forGP73, wherein the at least one capture antibody comprises: (a) avariable heavy domain comprising the amino acid sequence of SEQ IDNO:41; (b) a variable light domain comprising the amino acid sequence ofSEQ ID NO:45; (c) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:41 and a variable light domain comprising theamino acid sequence of SEQ ID NO:45; (d) a variable heavy chaincomprising a complementarity determining region (CDR)1 comprising theamino acid sequence of SEQ ID NO:42, a CDR2 comprising the amino acidsequence of SEQ ID NO:43, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:44; (e) a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:46, a CDR2 comprising the aminoacid sequence of SEQ ID NO:47, and a CDR3 comprising the amino acidsequence of SEQ ID NO:48; or (f) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:42, a CDR2comprising the amino acid sequence of SEQ ID NO:43, and a CDR3comprising the amino acid sequence of SEQ ID NO:44 and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:46, a CDR2 comprising the amino acid sequence of SEQ ID NO:47, and aCDR3 comprising the amino acid sequence of SEQ ID NO:48; wherein the atleast one detection antibody comprises: (i) variable heavy domaincomprising the amino acid sequence of SEQ ID NO:25; (ii) a variablelight domain comprising the amino acid sequence of SEQ ID NO:29; (iii) avariable heavy domain comprising the amino acid sequence of SEQ ID NO:25and a variable light domain comprising the amino acid sequence of SEQ IDNO:29; (iv) a variable heavy chain comprising a CDR1 comprising theamino acid sequence of SEQ ID NO:26, a CDR2 comprising the amino acidsequence of SEQ ID NO:27, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:28; (v) a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:30, a CDR2 comprising the aminoacid sequence of SEQ ID NO:31, and a CDR3 comprising the amino acidsequence of SEQ ID NO:32; or (vi) a variable heavy chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:26, a CDR2comprising the amino acid sequence of SEQ ID NO:27, and a CDR3comprising the amino acid sequence of SEQ ID NO:28 and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:30, a CDR2 comprising the amino acid sequence of SEQ ID NO:31, and aCDR3 comprising the amino acid sequence of SEQ ID NO:32, wherein the atleast one detection antibody is optionally detectably labeled.
 99. A kitfor assaying a test sample for fucosylated GP73, which kit comprises atleast one capture binding protein, wherein the capture binding proteinbinds to GP73 or a fragment of GP73, at least one detection bindingprotein, wherein the detection binding protein binds to GP73 that is notbound by the capture binding protein, and instructions for assaying thetest sample for GP73, wherein the at least one capture binding proteincomprises a protein, antibody or antibody fragment whose binding to GP73is insensitive to the presence or absence of a fucose sugar moiety onGP73 and the at least one detection binding protein comprises a protein,antibody or antibody fragment whose binding to GP73 is sensitive tofucosylation of GP73.
 100. A kit for assaying a test sample forfucosylated GP73, which kit comprises at least one capture bindingprotein, wherein the capture binding protein binds to GP73 or a fragmentof GP73, at least one detection binding protein, wherein the detectionbinding protein binds to GP73 that is not bound by the capture bindingprotein, and instructions for assaying the test sample for GP73, whereinthe at least one capture binding protein comprises a protein, antibodyor antibody fragment whose binding to GP73 is sensitive to fucosylationof GP73 and the at least one detection binding protein comprises aprotein, antibody or antibody fragment whose binding to GP73 isinsensitive to fucosylation of GP73.
 101. The kit of claim 96, whereinthe protein, antibody or antibody fragment whose binding to GP73 issensitive to fucosylation of GP73 comprises: (a) Aleuria aurantia lectin(AAL) or a fragment thereof; (b) a variable heavy domain regioncomprising the amino acid sequence of SEQ ID NO:25 or SEQ ID NO:33; (c)a variable light domain region comprising the amino acid sequence of SEQID NO:29 or SEQ ID NO:37; (d) a variable heavy domain comprising theamino acid sequence of SEQ ID NO:25 and a variable light domain regioncomprising the amino acid sequence of SEQ ID NO:29; (e) a variable heavydomain comprising the amino acid sequence of SEQ ID NO:33 and a variablelight domain region comprising the amino acid sequence of SEQ ID NO:37;(f) a variable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:26, a CDR2 comprising the amino acid sequence ofSEQ ID NO:27, and a CDR3 comprising the amino acid sequence of SEQ IDNO:28; (g) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:34, a CDR2 comprising the amino acid sequenceof SEQ ID NO:35, and a CDR3 comprising the amino acid sequence of SEQ IDNO:36; (h) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:30, a CDR2 comprising the amino acid sequenceof SEQ ID NO:31, and a CDR3 comprising the amino acid sequence of SEQ IDNO:32; (i) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:38, a CDR2 comprising the amino acid sequenceof SEQ ID NO:39, and a CDR3 comprising the amino acid sequence of SEQ IDNO:40; (j) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:26, a CDR2 comprising the amino acidsequence of SEQ ID NO:27, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:28, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:30, a CDR2 comprising the aminoacid sequence of SEQ ID NO:31, and a CDR3 comprising the amino acidsequence of SEQ ID NO:32; or (k) a variable heavy chain comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:34, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:35, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:36, and a variable light chain comprising aCDR1 comprising the amino acid sequence of SEQ ID NO:38, a CDR2comprising the amino acid sequence of SEQ ID NO:39, and a CDR3comprising the amino acid sequence of SEQ ID NO:40;
 102. The kit ofclaim 96, wherein the protein, antibody or antibody fragment whosebinding to GP73 is insensitive to fucosylation of GP73 comprises: (a) avariable heavy domain region comprising the amino acid sequence of SEQID NO:1, SEQ ID NO:9, SEQ ID NO:17, or SEQ ID NO:41; (b) a variablelight domain region comprising the amino acid sequence of SEQ ID NO:5,SEQ ID NO:13, SEQ ID NO:21, or SEQ ID NO:45; (c) a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:1 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:5; (d) avariable heavy domain comprising the amino acid sequence of SEQ ID NO:9and a variable light domain region comprising the amino acid sequence ofSEQ ID NO:13; (e) a variable heavy domain comprising the amino acidsequence of SEQ ID NO:17 and a variable light domain region comprisingthe amino acid sequence of SEQ ID NO:21; (f) a variable heavy domaincomprising the amino acid sequence of SEQ ID NO:41 and a variable lightdomain region comprising the amino acid sequence of SEQ ID NO:45; (g) avariable heavy chain comprising a CDR1 comprising the amino acidsequence of SEQ ID NO:2, a CDR2 comprising the amino acid sequence ofSEQ ID NO:3, and a CDR3 comprising the amino acid sequence of SEQ IDNO:4; (h) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:10, a CDR2 comprising the amino acid sequenceof SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ IDNO:12; (i) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:18, a CDR2 comprising the amino acid sequenceof SEQ ID NO:19, and a CDR3 comprising the amino acid sequence of SEQ IDNO:20; (j) a variable heavy chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequenceof SEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44; (k) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:6, a CDR2 comprising the amino acid sequenceof SEQ ID NO:7, and a CDR3 comprising the amino acid sequence of SEQ IDNO:8; (l) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:14, a CDR2 comprising the amino acid sequenceof SEQ ID NO:15, and a CDR3 comprising the amino acid sequence of SEQ IDNO:16; (m) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:22, a CDR2 comprising the amino acid sequenceof SEQ ID NO:23, and a CDR3 comprising the amino acid sequence of SEQ IDNO:24; (n) a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48; (o) a variable heavy chain comprising a CDR 1 comprising theamino acid sequence of SEQ ID NO:2, a CDR2 comprising the amino acidsequence of SEQ ID NO:3, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:4, and a variable light chain comprising a CDR1 comprisingthe amino acid sequence of SEQ ID NO:6, a CDR2 comprising the amino acidsequence of SEQ ID NO:7, and a CDR3 comprising the amino acid sequenceof SEQ ID NO:8; (p) a variable heavy chain comprising a CDR 1 comprisingthe amino acid sequence of SEQ ID NO:10, a CDR2 comprising the aminoacid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acidsequence of SEQ ID NO:12, and a variable light chain comprising a CDR1comprising the amino acid sequence of SEQ ID NO:14, a CDR2 comprisingthe amino acid sequence of SEQ ID NO:15, and a CDR3 comprising the aminoacid sequence of SEQ ID NO:16; (q) a variable heavy chain comprising aCDR 1 comprising the amino acid sequence of SEQ ID NO:18, a CDR2comprising the amino acid sequence of SEQ ID NO:19, and a CDR3comprising the amino acid sequence of SEQ ID NO:20, and a variable lightchain comprising a CDR1 comprising the amino acid sequence of SEQ IDNO:22, a CDR2 comprising the amino acid sequence of SEQ ID NO:23, and aCDR3 comprising the amino acid sequence of SEQ ID NO:24; or (r) avariable heavy chain comprising a CDR 1 comprising the amino acidsequence of SEQ ID NO:42, a CDR2 comprising the amino acid sequence ofSEQ ID NO:43, and a CDR3 comprising the amino acid sequence of SEQ IDNO:44, and a variable light chain comprising a CDR1 comprising the aminoacid sequence of SEQ ID NO:46, a CDR2 comprising the amino acid sequenceof SEQ ID NO:47, and a CDR3 comprising the amino acid sequence of SEQ IDNO:48.
 103. The kit of claim 95, further comprising a reference standardindicating a GP73 concentration in a control or calibrator.